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Literature summary for 1.1.1.202 extracted from

  • Gonzalez-Pajuelo, M.; Meynial-Salles, I.; Mendes, F.; Soucaille, P.; Vasconcelos, I.
    Microbial conversion of glycerol to 1,3-propanediol: physiological comparison of a natural producer, Clostridium butyricum VPI 3266, and an engineered strain, Clostridium acetobutylicum DG1(pSPD5) (2006), Appl. Environ. Microbiol., 72, 96-101.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Clostridium acetobutylicum mutant strain DG1 from plasmid pSPD5, subcloning in Escherichia coli Clostridium butyricum

Protein Variants

Protein Variants Comment Organism
nore expression of the Clostridumm butyricum enzyme in Clostridium acetobutylicum strain DG1(pSPD5) leads to functional reduction of glycerol to 1,3-propanediol in a two-step process, the first step of glycerol reduction to 3-hydroxypropionaldehyde, which is then reduced to 1,3-propanediol, is performed by the B12-independent glycerol dehydratase Clostridium butyricum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol + NADH Clostridium butyricum
-
 propane-1,3-diol + NAD+
-
 ?
glycerol + NADH Clostridium butyricum VPI 3266
-
 propane-1,3-diol + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium butyricum
-
-
-
Clostridium butyricum VPI 3266
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 nucleotide pools of wild-type Clostridium butyricum strain VPI 3266 and recombinant Clostridium acetobutylicum mutant strain DG1 Clostridium butyricum
0.37
-
 wild-type Clostridium butyricum strain VPI 3266 Clostridium butyricum
1.2
-
 recombinant enzyme in Clostridium acetobutylicum mutant strain DG1 Clostridium butyricum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropionaldehyde + NADH recombinant mutant Clostridium acetobutylicum strain DG1(pSPD5) expressing the enzyme from an introduced plasmid Clostridium butyricum propane-1,3-diol + NAD+
-
 ?
3-hydroxypropionaldehyde + NADH recombinant mutant Clostridium acetobutylicum strain DG1(pSPD5) expressing the enzyme from an introduced plasmid Clostridium butyricum VPI 3266 propane-1,3-diol + NAD+
-
 ?
glycerol + NADH
-
 Clostridium butyricum propane-1,3-diol + NAD+
-
 ?
glycerol + NADH
-
 Clostridium butyricum VPI 3266 propane-1,3-diol + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
1,3-Pdiol dehydrogenase
-
 Clostridium butyricum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Clostridium butyricum

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Clostridium butyricum