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Literature summary for 1.1.1.202 extracted from

  • Marcal, D.; Rego, A.T.; Fogg, M.J.; Wilson, K.S.; Carrondo, M.A.; Enguita, F.J.
    Crystallization and preliminary X-ray characterization of 1,3-propanediol dehydrogenase from the human pathogen Klebsiella pneumoniae (2007), Acta Crystallogr. Sect. F, 63, 249-251.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.202

Cloned(Commentary)

Cloned (Comment) Organism
gene dhaT, expression of the N-terminally His-tagged in Escherichia coli strain BL21(DE3) Klebsiella pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme, sitting-drop vapour diffusion, 22°C, 0.0015 ml of 55 mg/ml protein in 50 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM MnCl2 and 2 mM DTT, is mixed with an equal volume of reservoir solution containing 0.1 M MES, pH 6.5, with 12% w/v PEG 20000, and 10 mM CaCl2, 8-24 h, X-ray diffraction structure determination and analysis at 2.7 A resolution Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41544
-
 x * 41544, sequence calculation Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxypropanal + NADH + H+ Klebsiella pneumoniae a key enzyme in the reductive pathway of anaerobic glycerol dissimilation converting glycerol to 1,3-propanediol in the human pathogen Klebsiella pneumoniae propane-1,3-diol + NAD+
-
 r

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
 gene dhaT
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropanal + NADH + H+
-
 Klebsiella pneumoniae propane-1,3-diol + NAD+
-
 r
3-hydroxypropanal + NADH + H+ a key enzyme in the reductive pathway of anaerobic glycerol dissimilation converting glycerol to 1,3-propanediol in the human pathogen Klebsiella pneumoniae Klebsiella pneumoniae propane-1,3-diol + NAD+
-
 r

Subunits

Subunits Comment Organism
? x * 41544, sequence calculation Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
1,3-PD-DH
-
 Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Klebsiella pneumoniae
NADH
-
 Klebsiella pneumoniae

pI Value

Organism Comment pI Value Maximum pI Value
Klebsiella pneumoniae sequence calculation
-
 5.9