Literature summary for 1.1.1.202 extracted from

Wang, F.; Qu, H.; Zhang, D.; Tian, P.; Tan, T.
Production of 1,3-propanediol from glycerol by recombinant E. coli using incompatible plasmids system (2007), Mol. Biotechnol., 37, 112-119.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene dhaT, high level co-expression of the 1,3-PD oxidoreductase with glycerol dehydratase, encoded by gene dhaB, and glycerol dehydratase reactivating factor, encoded by gene gdrAB, in Escherichia coli strain BL21 (DE3) using two incompatible plasmids, fed-batch fermentation of recombinant bacteria. The NADPH-linked alcohol dehydrogenase, which is encoded by yqhD, a gene from Escherichia coli, can non-specifically catalyze 3-hydroxypropionaldehyde converting to 1,3-propanediol with sufficient activity, overview	Klebsiella pneumoniae

Cloned (Comment)

Organism

gene dhaT, high level co-expression of the 1,3-PD oxidoreductase with glycerol dehydratase, encoded by gene dhaB, and glycerol dehydratase reactivating factor, encoded by gene gdrAB, in Escherichia coli strain BL21 (DE3) using two incompatible plasmids, fed-batch fermentation of recombinant bacteria. The NADPH-linked alcohol dehydrogenase, which is encoded by yqhD, a gene from Escherichia coli, can non-specifically catalyze 3-hydroxypropionaldehyde converting to 1,3-propanediol with sufficient activity, overview

Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3-hydroxypropanal + NADH + H+	Klebsiella pneumoniae	-	propane-1,3-diol + NAD+	-	r

Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	-	strain DSM 2026, gene dhaT	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.8	-	native enzyme	Klebsiella pneumoniae
9.6	-	recombinant enzyme	Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-hydroxypropanal + NADH + H+	-	Klebsiella pneumoniae	propane-1,3-diol + NAD+	-	r

Synonyms

Synonyms	Comment	Organism
1,3-PD oxidoreductase	-	Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Klebsiella pneumoniae

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Klebsiella pneumoniae
NADH	-	Klebsiella pneumoniae