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EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Reference
2.4.2.64	0.00006	-	guanine	pH and temperature not specified in the publication	759447
2.4.2.64	0.00026	-	queuine	pH 7.3, 37°C, wild-type	721496
2.4.2.64	0.00026	-	queuine	pH 7.3, 37°C, wild-type enzyme	759860
2.4.2.64	0.00029	-	queuine	pH 7.5, 37°C	758771
2.4.2.64	0.00035	-	guanine	pH 7.3, 37°C, wild-type	721496
2.4.2.64	0.00036	-	guanine34 in tRNA	mutant enzyme Y106F	658446
2.4.2.64	0.00038	-	guanine	wild-type enzyme	658446
2.4.2.64	0.0006	-	queuine	recombinant enzyme whose subunits are produced separately and combined in a molar ratio of 1:1 for kinetic measurements, pH and temperature not specified in the publication	758814
2.4.2.64	0.00087	-	guanine	pH 7.3, 37°C, mutant C145V/T146V	721496
2.4.2.64	0.001	-	guanine	pH 7.3, 37°C, mutant V217G	721496

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Release 2023.1 (January 2023)