Any feedback?
Literature summary for 2.4.2.64 extracted from
- Flexible adaptations in the structure of the tRNA-modifying enzyme tRNA-guanine transglycosylase and their implications for substrate selectivity, reaction mechanism and structure-based drug design (2003), ChemBioChem, 4, 1066-1077.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| wild-type and mutant enzyme Y106F, hanging drop method, crystals with a size of approximately 0.7 * 0.7 * 0.2 mm grew within five to seven days | Zymomonas mobilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y106F | 2.8fold decrease in turnover-number for guanine and tRNA, 2.8fold decrease in Km-value for tRNA, 3.4fold increase in turnover-number for guanine, in contrast to Phe106 the residue Tyr106 is hydrogen bonded in the ligand-free structure, this is not the reason for the kinetic difference, other structurally less evident factors are responsible for this behaviour | Zymomonas mobilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-diamino-8-[[(1H-imidazol-2-yl)sulfanyl]methyl]quinazolin-4(3H)-one | - |
Zymomonas mobilis |  |
| 2-butyl-5,6-dihydro-1H-imidazo[4,5-d]pyridazine-4,7-dione | - |
Zymomonas mobilis | |
| 4-aminophthalhydrazide | - |
Zymomonas mobilis | |
| 5,7-diamino-2,3-dihydro-phthalazine-1,4-dione | - |
Zymomonas mobilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00036 | - |
guanine34 in tRNA | mutant enzyme Y106F | Zymomonas mobilis | |
| 0.00038 | - |
guanine | wild-type enzyme | Zymomonas mobilis | |
| 0.001 | - |
guanine34 in tRNA | wild-type enzyme | Zymomonas mobilis | |
| 0.0013 | - |
guanine | mutant enzyme Y106F | Zymomonas mobilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine34 in tRNA + queuine | Zymomonas mobilis | catalyses a base-exchange reaction that leads to anticodon modifications of certain tRNAs | queuine34 in tRNA + guanine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zymomonas mobilis | P28720 | wild-type and Y106F mutant | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine34 in tRNA + queuine | - |
Zymomonas mobilis | queuine34 in tRNA + guanine | - |
? | |
| guanine34 in tRNA + queuine | catalyses a base-exchange reaction that leads to anticodon modifications of certain tRNAs | Zymomonas mobilis | queuine34 in tRNA + guanine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TGT | - |
Zymomonas mobilis |
| tRNA-guanine transglycosylase | - |
Zymomonas mobilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
guanine | mutant enzyme Y106F | Zymomonas mobilis | |
| 0.005 | - |
guanine34 in tRNA | mutant enzyme Y106F | Zymomonas mobilis | |
| 0.011 | - |
guanine | wild-type enzyme | Zymomonas mobilis | |
| 0.014 | - |
guanine34 in tRNA | wild-type enzyme | Zymomonas mobilis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
