Literature summary for 2.4.2.64 extracted from

Shindo-Okada, N.; Okada, N.; Ohgi, T.; Goto, T.; Nishimura, S.
Transfer ribonucleic acid guanine transglycosylase isolated from rat liver (1980), Biochemistry, 19, 395-400 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00029	-	queuine	pH 7.5, 37°C	Rattus norvegicus
0.0021	-	7-(aminomethyl)-7-deazaguanine	pH 7.5, 37°C	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	not stimulated by Mg2+	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	gel filtration	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
guanine34 in tRNA + queuine	Rattus norvegicus	-	queuine34 in tRNA + guanine	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-
Morris hepatoma 7316A cell	-	Rattus norvegicus	-

Storage Stability

Storage Stability	Organism
-20°C, 50% glycerol, no loss of activity after 10 months	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
guanine34 in tRNA + 7-(aminomethyl)-7-deazaguanine	the affinity of the rat liver tRNA-guanine transglycosylase for queuine is much greater than that for 7-(aminomethyl)-7-deazaguanine and guanine	Rattus norvegicus	7-(aminomethyl)-7-deazaguanine34 in tRNA + guanine	-	?
guanine34 in tRNA + queuine	-	Rattus norvegicus	queuine34 in tRNA + guanine	-	?
guanine34 in tRNA + queuine	the affinity of the rat liver tRNA-guanine transglycosylase for queuine is much greater than that for 7-(aminomethyl)-7-deazaguanine and guanine	Rattus norvegicus	queuine34 in tRNA + guanine	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.3	-	-	Rattus norvegicus

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	7.5	pH 7.0: more than 80% of maximal activity, pH 7.5: more than 80% of maximal activity	Rattus norvegicus

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Release 2023.1 (January 2023)