Any feedback?
Literature summary for 2.4.2.64 extracted from
- Purification and properties of guanine, queuine-tRNA transglycosylase from wheat germ (1982), J. Biol. Chem., 257, 13218-13222 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 7-(aminomethyl)-7-deazaguanine | 0.01 mM, 28% inhibition | Triticum aestivum |  |
| Cd2+ | 10 mM | Triticum aestivum | |
| Co2+ | 10 mM | Triticum aestivum | |
| Cu2+ | 10 mM | Triticum aestivum | |
| Mn2+ | 10 mM | Triticum aestivum | |
| Ni2+ | 10 mM | Triticum aestivum | |
| Pb2+ | 10 mM | Triticum aestivum | |
| queuine | - |
Triticum aestivum | |
| Zn2+ | 10 mM | Triticum aestivum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00006 | - |
guanine | pH and temperature not specified in the publication | Triticum aestivum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 1 mM, less effective than Mg2+ in meeting the divalent ion requirement | Triticum aestivum | |
| Ca2+ | 1 mM, less effective than Mg2+ in meeting the divalent ion requirement | Triticum aestivum | |
| Cs+ | less effective than Na+ in meeting the monovalent ion requirement | Triticum aestivum | |
| K+ | less effective than Na+ in meeting the monovalent ion requirement | Triticum aestivum | |
| Li+ | less effective than Na+ in meeting the monovalent ion requirement | Triticum aestivum | |
| Mg2+ | the enzyme requires a monovalent or a divalent cation. Mg2+ is most effective in meeting the divalent cation requirement. Optimal Mg2+ concentration is 5.0 mM | Triticum aestivum | |
| Na+ | the enzyme requires a monovalent or a divalent cation. Na+ is most effective in meeting the monovalent requirement. Optimal concentration is 114 mM | Triticum aestivum | |
| NaCl | optimum concentration is 114 mM | Triticum aestivum | |
| Rb+ | less effective than Na+ in meeting the monovalent ion requirement | Triticum aestivum | |
| Sr2+ | 1 mM, less effective than Mg2+ in meeting the divalent ion requirement | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | - |
gel filtration | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine34 in tRNA + queuine | Triticum aestivum | - |
queuine34 in tRNA + guanine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| plant embryo | - |
Triticum aestivum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine34 in tRNA + queuine | - |
Triticum aestivum | queuine34 in tRNA + guanine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 68000, SDS-PAGE | Triticum aestivum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| guanine,queuine-tRNA transglycosylase | - |
Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Triticum aestivum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000095 | - |
queuine | pH and temperature not specified in the publication | Triticum aestivum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
