EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference  |
|---|
    1.8.4.9 | 1.12 |
- |
glutathione |
about, holoenzyme APR1p and C-domain with substrates hydroxyethyldisulfide, or cystine, pH 8.0, 24°C |
660399 |
| 1.8.4.9 | 1.15 |
- |
glutathione |
about, holoenzyme APR1p and C-domain with substrate dehydroascorbate, pH 6.9, 24°C |
660399 |
| 1.8.4.9 | 0.00057 |
- |
5'-adenylylsulfate |
assay conditions: APS reductase (0.00021 mM), PK (8.4 units/ml), LDH (18.0 units/ml), adenylate kinase (10 units/ml), thioredoxin (0.0373 mM), MgCl2 (3.0 mM), DTT (2.0 mM), ATP (1.0 mM), PEP (1.0 mM), EDTA (1.0 mM), GSH (5.0 mM), NADH (0.030 mM), and Hepes (50 mM, pH 8.0), at 25°C |
667703 |
| 1.8.4.9 | 0.031 |
- |
3'-phosphoadenosine-5'-phosphosulfate |
assay conditions: APS reductase (0.0009 mM), EST (0.10 unit/ml), thioredoxin (0.037 mM), MgCl2 (3.0 mM), DTT (2.0 mM), pNPS (10 mM), GSH (5.0 mM), and Hepes (50 mM, pH 8.0), at 25°C |
667703 |
| 1.8.4.9 | 0.0002 |
- |
glutathione |
- |
667746 |
| 1.8.4.9 | 0.011 |
- |
5'-adenylyl sulfate |
- |
667746 |
| 1.8.4.9 | 0.0033 |
- |
5'-adenylylsulfate |
in the presence of 0.001 mM thioredoxin |
669891 |
| 1.8.4.9 | 0.0052 |
- |
5'-adenylylsulfate |
in the presence of 0.01 mM thioredoxin |
669891 |
| 1.8.4.9 | 0.02 |
- |
3'-phosphoadenosine 5'-phosphosulfate |
- |
687585 |
| 1.8.4.9 | 0.05 |
- |
adenosine 5'-phosphosulfate |
- |
687585 |
