1.8.4.9 0.0025 - 5'-adenylylsulfate with glutathione as reductant 288642 1.8.4.9 0.0038 - 5'-adenylylsulfate with DTT as reductant 288642 1.8.4.9 0.4 - DTT with 5'-adenylylsulfate as substrate 288642 1.8.4.9 3 - glutathione with 5'-adenylylsulfate as substrate 288642 1.8.4.9 0.0065 - 5'-adenylylsulfate with glutathione as reductant 288643 1.8.4.9 1.39 - glutathione with 5'-adenylylsulfate as substrate 288643 1.8.4.9 0.0065 - 5'-adenylylsulfate with 10 mM glutathione as reductant 288644 1.8.4.9 0.0122 - 5'-adenylylsulfate with 40 mM glutathione as reductant 288644 1.8.4.9 16.8 - glutathione with 0.04 mM adenylylsulfate 288644 1.8.4.9 0.6 - glutathione holoenzyme APR1p with substrate 5'-adenylyl sulfate, pH 8.5, 30°C 660399 1.8.4.9 1.12 - glutathione about, holoenzyme APR1p and C-domain with substrates hydroxyethyldisulfide, or cystine, pH 8.0, 24°C 660399 1.8.4.9 1.15 - glutathione about, holoenzyme APR1p and C-domain with substrate dehydroascorbate, pH 6.9, 24°C 660399 1.8.4.9 0.00057 - 5'-adenylylsulfate assay conditions: APS reductase (0.00021 mM), PK (8.4 units/ml), LDH (18.0 units/ml), adenylate kinase (10 units/ml), thioredoxin (0.0373 mM), MgCl2 (3.0 mM), DTT (2.0 mM), ATP (1.0 mM), PEP (1.0 mM), EDTA (1.0 mM), GSH (5.0 mM), NADH (0.030 mM), and Hepes (50 mM, pH 8.0), at 25°C 667703 1.8.4.9 0.031 - 3'-phosphoadenosine-5'-phosphosulfate assay conditions: APS reductase (0.0009 mM), EST (0.10 unit/ml), thioredoxin (0.037 mM), MgCl2 (3.0 mM), DTT (2.0 mM), pNPS (10 mM), GSH (5.0 mM), and Hepes (50 mM, pH 8.0), at 25°C 667703 1.8.4.9 0.0002 - glutathione - 667746 1.8.4.9 0.011 - 5'-adenylyl sulfate - 667746 1.8.4.9 0.0033 - 5'-adenylylsulfate in the presence of 0.001 mM thioredoxin 669891 1.8.4.9 0.0052 - 5'-adenylylsulfate in the presence of 0.01 mM thioredoxin 669891 1.8.4.9 0.02 - 3'-phosphoadenosine 5'-phosphosulfate - 687585 1.8.4.9 0.05 - adenosine 5'-phosphosulfate - 687585 1.8.4.9 0.0112 - 5'-adenylyl sulfate 30°C, pH 8.5, cosubstrate: dithiothreitol 689431 1.8.4.9 0.0174 - 5'-adenylyl sulfate 30°C, pH 8.5, cosubstrate: glutathione 689431 1.8.4.9 0.495 - glutathione 30°C, pH 8.5 689431 1.8.4.9 0.595 - dithiothreitol 30°C, pH 8.5 689431 1.8.4.9 additional information - additional information thermodynamic parameters for binding of AtAPR1 redox domain to GSH or GSSG 763921