BRENDA - Enzyme Database show
show all sequences of 1.8.4.9

Glutaredoxin function for the carboxyl-terminal domain of the plant-type 5'-adenylylsulfate reductase

Bick, J.A.; Aeslund, F.; Chen, Y.; Leustek, T.; Proc. Natl. Acad. Sci. USA 95, 8404-8409 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene APR1, expression of the holoenzyme APR1p in Escherichia coli strain BL21(DE3), separate expression in Escherichia coli strain BL21(DE3) of amino acid residues 73-327, forming the R-domain, and of residues 328-465, forming the C-domain, the domains alone are inactive, but mixing of both can partially restore activity
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
gene APR1, the holoenzyme APR1p as well as the C-domain alone can complement the cysteine auxotrophy of an Escherichia coli cysH mutant strain, substituting for glutaredoxin, if the mutant is capable of producing glutathione, cysH encodes the 5'-phosphoadenylylsulfate reductase in Escherichia coli
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
glutathione
slightly inhibitory at high concentrations
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.6
-
glutathione
holoenzyme APR1p with substrate 5'-adenylyl sulfate, pH 8.5, 30°C
Arabidopsis thaliana
1.12
-
glutathione
about, holoenzyme APR1p and C-domain with substrates hydroxyethyldisulfide, or cystine, pH 8.0, 24°C
Arabidopsis thaliana
1.15
-
glutathione
about, holoenzyme APR1p and C-domain with substrate dehydroascorbate, pH 6.9, 24°C
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Arabidopsis thaliana
enzyme is involved in sulfate assimilation
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
P92979
APR1; 3 genes APR1, APR2, and APR3, 3 isozymes APR1p, APR2p, and APR3p
-
Purification (Commentary)
Commentary
Organism
recombinant holoenzyme APR1p, C-domain, and R-domain from Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-adenylyl sulfate + glutathione
catalyzed by the holoenzyme APR1p, not by the C-domain
660399
Arabidopsis thaliana
AMP + sulfite + glutathione disulfide
-
-
-
?
cystine + glutathione
catalyzed by the holoenzyme APR1p and the C-domain
660399
Arabidopsis thaliana
cysteine + glutathione disulfide
-
-
-
?
dehydroascorbate + glutathione
catalyzed by the holoenzyme APR1p and the C-domain
660399
Arabidopsis thaliana
ascorbate + glutathione disulfide
-
-
-
?
hydroxyethyldisulfide + glutathione
catalyzed by the holoenzyme APR1p and the C-domain
660399
Arabidopsis thaliana
?
-
-
-
?
insulin disulfide + glutathione
catalyzed by the C-domain, not by the holoenzyme APR1p or the R-domain
660399
Arabidopsis thaliana
insulin + glutathione disulfide
-
-
-
?
additional information
enzyme is involved in sulfate assimilation
660399
Arabidopsis thaliana
?
-
-
-
-
oxidized ribonucleotide reductase + glutathione
catalyzed by the C-domain, not by the holoenzyme APR1p or the R-domain
660399
Arabidopsis thaliana
reduced ribonucleotide reductase + glutathione disulfide
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
amino acid residues 73-327 form the R-domain which is homologous to microbial 5'-phosphoadenylylsulfate reductase, and of residues 328-465 form the C-domain which is homologous to thioredoxin, the domains alone are inactive, but mixing of both can partially rstore activity
Arabidopsis thaliana
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
24
30
-
Arabidopsis thaliana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
-
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
glutathione
dependent on, specific cofactor for the carboxyterminal glutathione-dependent reductase domain of the enzyme, can be exchanged for equally active DTT, glutathione is slightly inhibitory at high concentrations
Arabidopsis thaliana
additional information
DTT can be exchanged for glutathione, equally active; no activity with 2-mercaptoethanol, gamma-glutamylcysteine, or cysteine
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
gene APR1, expression of the holoenzyme APR1p in Escherichia coli strain BL21(DE3), separate expression in Escherichia coli strain BL21(DE3) of amino acid residues 73-327, forming the R-domain, and of residues 328-465, forming the C-domain, the domains alone are inactive, but mixing of both can partially restore activity
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
glutathione
dependent on, specific cofactor for the carboxyterminal glutathione-dependent reductase domain of the enzyme, can be exchanged for equally active DTT, glutathione is slightly inhibitory at high concentrations
Arabidopsis thaliana
additional information
DTT can be exchanged for glutathione, equally active; no activity with 2-mercaptoethanol, gamma-glutamylcysteine, or cysteine
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
gene APR1, the holoenzyme APR1p as well as the C-domain alone can complement the cysteine auxotrophy of an Escherichia coli cysH mutant strain, substituting for glutaredoxin, if the mutant is capable of producing glutathione, cysH encodes the 5'-phosphoadenylylsulfate reductase in Escherichia coli
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
glutathione
slightly inhibitory at high concentrations
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.6
-
glutathione
holoenzyme APR1p with substrate 5'-adenylyl sulfate, pH 8.5, 30°C
Arabidopsis thaliana
1.12
-
glutathione
about, holoenzyme APR1p and C-domain with substrates hydroxyethyldisulfide, or cystine, pH 8.0, 24°C
Arabidopsis thaliana
1.15
-
glutathione
about, holoenzyme APR1p and C-domain with substrate dehydroascorbate, pH 6.9, 24°C
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Arabidopsis thaliana
enzyme is involved in sulfate assimilation
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant holoenzyme APR1p, C-domain, and R-domain from Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-adenylyl sulfate + glutathione
catalyzed by the holoenzyme APR1p, not by the C-domain
660399
Arabidopsis thaliana
AMP + sulfite + glutathione disulfide
-
-
-
?
cystine + glutathione
catalyzed by the holoenzyme APR1p and the C-domain
660399
Arabidopsis thaliana
cysteine + glutathione disulfide
-
-
-
?
dehydroascorbate + glutathione
catalyzed by the holoenzyme APR1p and the C-domain
660399
Arabidopsis thaliana
ascorbate + glutathione disulfide
-
-
-
?
hydroxyethyldisulfide + glutathione
catalyzed by the holoenzyme APR1p and the C-domain
660399
Arabidopsis thaliana
?
-
-
-
?
insulin disulfide + glutathione
catalyzed by the C-domain, not by the holoenzyme APR1p or the R-domain
660399
Arabidopsis thaliana
insulin + glutathione disulfide
-
-
-
?
additional information
enzyme is involved in sulfate assimilation
660399
Arabidopsis thaliana
?
-
-
-
-
oxidized ribonucleotide reductase + glutathione
catalyzed by the C-domain, not by the holoenzyme APR1p or the R-domain
660399
Arabidopsis thaliana
reduced ribonucleotide reductase + glutathione disulfide
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
amino acid residues 73-327 form the R-domain which is homologous to microbial 5'-phosphoadenylylsulfate reductase, and of residues 328-465 form the C-domain which is homologous to thioredoxin, the domains alone are inactive, but mixing of both can partially rstore activity
Arabidopsis thaliana
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
24
30
-
Arabidopsis thaliana
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
-
Arabidopsis thaliana
Other publictions for EC 1.8.4.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743157
Chung
-
The Arabidopsis thaliana aden ...
Arabidopsis thaliana
J. Plant Biol.
58
128-136
2015
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1
1
1
1
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689431
Phartiyal
The role of 5-adenylylsulfate ...
Glycine max
Phytochemistry
69
356-364
2008
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1
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2
4
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1
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4
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4
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1
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4
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694712
Koprivova
Complex signaling network in r ...
Arabidopsis thaliana
Plant Physiol.
146
1408-1420
2008
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1
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667746
Kim
The two-domain structure of 5- ...
Ulva intestinalis
Biochemistry
46
591-601
2007
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668650
Duperron
Evidence for chemoautotrophic ...
bacteria endosymbiont of Lucinoma aff. kazani
FEMS Microbiol. Ecol.
59
64-70
2007
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1
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687585
Kopriva
The putative moss 3'-phosphoad ...
Physcomitrella patens
J. Biol. Chem.
282
22930-22938
2007
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1
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3
2
1
7
2
2
-
8
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1
1
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4
8
1
1
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2
2
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2
1
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1
2
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3
1
2
1
7
2
2
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1
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4
8
1
1
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2
2
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694695
Wiedemann
The role of the novel adenosin ...
Physcomitrella patens
Plant Mol. Biol.
65
667-676
2007
-
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1
-
1
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2
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1
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1
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2
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1
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2
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1
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1
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667703
Sun
Channeling in sulfate activati ...
Mycobacterium tuberculosis
Biochemistry
45
11304-11311
2006
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1
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2
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1
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2
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2
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667717
Schiffer
Reaction mechanism of the iron ...
Archaeoglobus fulgidus
Biochemistry
45
2960-2967
2006
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1
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2
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2
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1
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1
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669891
Chartron
Substrate recognition, protein ...
Pseudomonas aeruginosa
J. Mol. Biol.
364
152-169
2006
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1
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1
2
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1
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2
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1
1
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670319
Senaratne
5-Adenosinephosphosulphate red ...
Mycobacterium tuberculosis
Mol. Microbiol.
59
1744-1753
2006
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660168
Tsakraklides
Sulfate reduction is increased ...
Arabidopsis thaliana, Pseudomonas aeruginosa
Plant J.
32
879-889
2002
1
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1
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660113
Kopriva
-
Influence of chilling stress o ...
Zea mays
Plant Biol.
3
24-31
2001
1
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288643
Gao
Characterization of sulfate as ...
Arabidopsis thaliana, Brassica juncea, Conticribra weissflogii, Dunaliella salina, Emiliania huxleyi, Heterocapsa triquetra, Isochrysis galbana, Tetraselmis sp., Thalassiosira oceanica, Ulva intestinalis
Plant Physiol.
123
1087-1096
2000
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10
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12
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20
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1
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288644
Suter
Adenosine 5'-phosphosulfate su ...
Lemna minor
J. Biol. Chem.
275
930-936
2000
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1
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1
3
3
1
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3
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1
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1
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3
1
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1
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288642
Prior
Structural and kinetic propert ...
Catharanthus roseus
Biochim. Biophys. Acta
1430
25-38
1999
1
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1
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1
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660399
Bick
Glutaredoxin function for the ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
95
8404-8409
1998
-
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1
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1
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1
3
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1
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3
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1
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1
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7
1
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1
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1
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1
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1
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1
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1
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1
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7
1
-
1
-
-
-
1
-
-
-
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-
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-
288640
Setya
Sulfate reduction in higher pl ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
93
13383-13388
1996
3
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1
-
-
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1
-
1
-
3
-
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1
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-
-
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-
-
2
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3
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1
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3
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1
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1
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1
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3
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-
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-
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2
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3
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1
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288641
Gutierrez-Marcos
Three members of a novel small ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
93
13377-13382
1996
-
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1
-
-
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1
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4
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6
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1
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3
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2
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1
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1
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4
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1
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3
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2
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