EC Number   |
General Stability |
Reference |
|---|
   3.2.1.114 | 4-nitrophenyl alpha-D-mannoside stabilizes |
136013 |
| 3.2.1.114 | enzyme retains full activity after incubation with chymotrypsin or other proteases that cleave the enzyme into catalytic and membrane-anchoring domains, a decrease in activity occurs with a corresponding cleavage of the catalytic subunit to a MW 105000 species |
646623 |
| 3.2.1.114 | freezing and thawing greatly enhances the rate of cleavage of the intact enzyme |
135971 |
| 3.2.1.114 | freezing inactivates column-bound glutathione S-transferase-tagged secreted form of SfManIII |
646644 |
| 3.2.1.114 | glycerol stabilizes |
646582, 646626 |
| 3.2.1.114 | requires presence of detergent, 0.1% Triton X-100, to maintain enzyme in soluble form |
136013 |
| 3.2.1.114 | stable to repeated freeze-thawing cycles |
136013 |
| 3.2.1.114 | ultrafiltration, even in the presence of PMSF at 0°C, results in a slow conversion of the intact enzyme to the 110 kDa degradation product, the catalytic domain, which itself is extremely stable |
135971 |
| 3.2.1.114 | unfolding and denaturation in 6 M guanidinium hydrochloride, the protein almost completely unfolds in 4.0 M guanidinium hydrochloride, overview |
709324 |
