General Stability | Organism |
---|---|
enzyme retains full activity after incubation with chymotrypsin or other proteases that cleave the enzyme into catalytic and membrane-anchoring domains, a decrease in activity occurs with a corresponding cleavage of the catalytic subunit to a MW 105000 species | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | topology study: integral membrane protein with lumenal orientation, hydrophilic catalytic domain is bound to an integral hydrophobic membrane anchoring domain through a proteolytically sensitive linkage | Rattus norvegicus | 139 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
x * 110000 + x * 14000, 110 kDa proteolytically resistant, hydrophilic catalytic domain and 14 kDa hydrophobic membrane anchoring domain, generated by mild chymotrypsin digest, SDS-PAGE | Rattus norvegicus |
110000 | - |
x * 110000 + x * 14000, 110 kDa proteolytically resistant, hydrophilic catalytic domain and 14 kDa hydrophobic membrane anchoring domain, generated by mild chymotrypsin digest, SDS-PAGE | Rattus norvegicus |
124000 | - |
x * 124000, intact subunit, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | involved in asparagine-linked oligosaccharide processing | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl alpha-D-mannoside + H2O | - |
Rattus norvegicus | 4-methylumbelliferone + alpha-D-mannose | - |
? | |
4-nitrophenyl alpha-D-mannoside + H2O | - |
Rattus norvegicus | 4-nitrophenol + alpha-D-mannose | - |
? | |
additional information | involved in asparagine-linked oligosaccharide processing | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 110000 + x * 14000, 110 kDa proteolytically resistant, hydrophilic catalytic domain and 14 kDa hydrophobic membrane anchoring domain, generated by mild chymotrypsin digest, SDS-PAGE | Rattus norvegicus |
? | x * 124000, intact subunit, SDS-PAGE | Rattus norvegicus |