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Literature summary for 3.2.1.114 extracted from

  • Moremen, K.W.; Touster, O.
    Topology of mannosidase II in rat liver Golgi membranes and release of the catalytic domain by selective proteolysis (1986), J. Biol. Chem., 261, 10945-10951.
    View publication on PubMed

General Stability

General Stability Organism
enzyme retains full activity after incubation with chymotrypsin or other proteases that cleave the enzyme into catalytic and membrane-anchoring domains, a decrease in activity occurs with a corresponding cleavage of the catalytic subunit to a MW 105000 species Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane topology study: integral membrane protein with lumenal orientation, hydrophilic catalytic domain is bound to an integral hydrophobic membrane anchoring domain through a proteolytically sensitive linkage Rattus norvegicus 139
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
x * 110000 + x * 14000, 110 kDa proteolytically resistant, hydrophilic catalytic domain and 14 kDa hydrophobic membrane anchoring domain, generated by mild chymotrypsin digest, SDS-PAGE Rattus norvegicus
110000
-
x * 110000 + x * 14000, 110 kDa proteolytically resistant, hydrophilic catalytic domain and 14 kDa hydrophobic membrane anchoring domain, generated by mild chymotrypsin digest, SDS-PAGE Rattus norvegicus
124000
-
x * 124000, intact subunit, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus involved in asparagine-linked oligosaccharide processing ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-D-mannoside + H2O
-
Rattus norvegicus 4-methylumbelliferone + alpha-D-mannose
-
?
4-nitrophenyl alpha-D-mannoside + H2O
-
Rattus norvegicus 4-nitrophenol + alpha-D-mannose
-
?
additional information involved in asparagine-linked oligosaccharide processing Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
? x * 110000 + x * 14000, 110 kDa proteolytically resistant, hydrophilic catalytic domain and 14 kDa hydrophobic membrane anchoring domain, generated by mild chymotrypsin digest, SDS-PAGE Rattus norvegicus
? x * 124000, intact subunit, SDS-PAGE Rattus norvegicus