Cloned (Comment) | Organism |
---|---|
alpha-mannosidase III cDNA is cloned | Spodoptera frugiperda |
General Stability | Organism |
---|---|
freezing inactivates column-bound glutathione S-transferase-tagged secreted form of SfManIII | Spodoptera frugiperda |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | alpha-mannosidase III, 1 mM, 90% inhibition of 4-nitrophenyl alpha-D-mannoside hydrolysis, 5 mM, complete inhibition of (Man)5(GlcNAc)2-PA hydrolysis | Spodoptera frugiperda | |
swainsonine | alpha-mannosidase III, IC50: 10 nM | Spodoptera frugiperda | |
Zn2+ | alpha-mannosidase III | Spodoptera frugiperda |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | alpha-mannosidase III, integral membrane glycoprotein with type II topology | Spodoptera frugiperda | 139 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation | Spodoptera frugiperda | |
Co2+ | alpha-mannosidase III, cobalt-dependent | Spodoptera frugiperda | |
Mn2+ | alpha-mannosidase III, activates to a lesser extend than Co2+ | Spodoptera frugiperda | |
Ni2+ | alpha-mannosidase III, little activation | Spodoptera frugiperda |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
x * 150000, intact glutathione S-transferase-tagged secreted form of SfManIII, SDS-PAGE | Spodoptera frugiperda |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Spodoptera frugiperda | alpha-mannosidase III probably provides an alternate N-glycan processing pathway in Sf-9 cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spodoptera frugiperda | - |
Sf-9, alpha-mannosidase III | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | integral membrane glycoprotein | Spodoptera frugiperda |
Purification (Comment) | Organism |
---|---|
glutathione S-transferase-tagged secreted form of SfManIII | Spodoptera frugiperda |
Storage Stability | Organism |
---|---|
4°C, column-bound glutathione S-transferase-tagged secreted form of SfManIII, at least 1 week, stable | Spodoptera frugiperda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-mannoside + H2O | alpha-mannosidase III | Spodoptera frugiperda | 4-nitrophenol + alpha-D-mannose | - |
? | |
additional information | alpha-mannosidase III hydrolyzes (Man)5-9(GlcNAc)2, not: GlcNAc(Man)5(GlcNAc)2 | Spodoptera frugiperda | ? | - |
? | |
additional information | alpha-mannosidase III probably provides an alternate N-glycan processing pathway in Sf-9 cells | Spodoptera frugiperda | ? | - |
? | |
pyridylaminated (Man)5(GlcNAc)2 + H2O | via a (Man)4(GlcNAc)2 intermediate | Spodoptera frugiperda | pyridylaminated (Man)3(GlcNAc)2 + alpha-D-mannose | - |
? | |
pyridylaminated (Man)5(GlcNAc)2 + H2O | alpha-mannosidase III | Spodoptera frugiperda | pyridylaminated (Man)3(GlcNAc)2 + alpha-D-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 150000, intact glutathione S-transferase-tagged secreted form of SfManIII, SDS-PAGE | Spodoptera frugiperda |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Spodoptera frugiperda |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 6.7 | - |
Spodoptera frugiperda |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.7 | alpha-mannosidase III activity is negligible at pH 5.5, increases at pH 5.7-5.9, decreases at pH 6.1 and rises to a plateau at pH 6.5-6.7 | Spodoptera frugiperda |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00001 | - |
alpha-mannosidase III, IC50: 10 nM | Spodoptera frugiperda | swainsonine |