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Literature summary for 3.2.1.114 extracted from

  • Moremen, K.W.; Touster, O.; Robbins, P.W.
    Novel purification of the catalytic domain of golgi alpha-mannosidase II. Characterization and comparison with the intact enzyme (1991), J. Biol. Chem., 266, 16876-16885.
    View publication on PubMed
Search for more literature for 3.2.1.114

General Stability

General Stability Organism
freezing and thawing greatly enhances the rate of cleavage of the intact enzyme Rattus norvegicus
ultrafiltration, even in the presence of PMSF at 0°C, results in a slow conversion of the intact enzyme to the 110 kDa degradation product, the catalytic domain, which itself is extremely stable Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
swainsonine
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
40
-
 4-nitrophenyl alpha-D-mannoside intact 124 kDa Man II and chymotrypsin-cleaved 110 kDa catalytic subunit Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane integral membrane protein, hydrophilic catalytic domain is anchored to the lumenal face of Golgi membranes through an NH2-terminal hydrophobic membrane-anchoring domain Rattus norvegicus 139
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 2 * 110000, chymotrypsin-cleaved catalytic subunit, SDS-PAGE Rattus norvegicus
124000
-
 2 * 124000, intact enzyme, SDS-PAGE Rattus norvegicus
230000
-
 chymotrypsin-cleaved 110 kDa catalytic subunit, gel filtration Rattus norvegicus
285000
-
 intact enzyme, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GlcNAc(Man)5GlcNAc + 2 H2O Rattus norvegicus natural substrate, involved in the processing of N-linked oligosaccharides GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male sprague-dawley rats
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
intact 124 kDa Man II and chymotrypsin-cleaved soluble 110 kDa catalytic subunit Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.75
-
 intact 124 kDa Man II Rattus norvegicus
7.03
-
 chymotrypsin-cleaved soluble 110 kDa catalytic subunit Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, intact enzyme, several weeks, stable Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-mannoside + H2O
-
 Rattus norvegicus 4-nitrophenol + alpha-D-mannose
-
 ?
GlcNAc(Man)5GlcNAc + 2 H2O
-
 Rattus norvegicus GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose
-
 ?
GlcNAc(Man)5GlcNAc + 2 H2O natural substrate, involved in the processing of N-linked oligosaccharides Rattus norvegicus GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose
-
 ?
additional information domain structure Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 124000, intact enzyme, SDS-PAGE Rattus norvegicus
dimer 2 * 110000, chymotrypsin-cleaved catalytic subunit, SDS-PAGE Rattus norvegicus
dimer disulfide-linked homodimer Rattus norvegicus