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The taxonomic range for the selected organisms is: Thermus thermophilus
The enzyme appears in selected viruses and cellular organisms
Synonyms
arginyl-trna synthetase, argrs, rars2, arg-trna synthetase, mtargrs, arginine-trna synthetase, arginyl-transfer rna synthetase, args2, arginine-trna ligase,
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Arginine translase
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Arginine--tRNA ligase
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Arginine-tRNA synthetase
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Arginyl transfer ribonucleic acid synthetase
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Arginyl-transfer RNA synthetase
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Arginyl-tRNA synthetase
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Synthetase, arginyl-transfer ribonucleate
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L-arginine:tRNAArg ligase (AMP-forming)
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ATP + L-arginine + tRNAArg
AMP + diphosphate + L-arginyl-tRNAArg
ATP + L-arginine + tRNAArg
AMP + diphosphate + L-arginyl-tRNAArg
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ATP + L-arginine + tRNAArg
AMP + diphosphate + L-arginyl-tRNAArg
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ATP + L-arginine + tRNAArg
AMP + diphosphate + L-arginyl-tRNAArg
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0.0098
tRNAArg
adenine at position 20 of the tRNAArg is required for activity, pH 7.5, 65°C
additional information
additional information
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additional information
additional information
Km values for the wild type and mutant enzymes using tRNAArg with different nucleotides at position 20
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additional information
additional information
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Km values for the wild type and mutant enzymes using tRNAArg with different nucleotides at position 20
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additional information
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K116G mutant displays very low activity compared to wild type enzyme
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SwissProt
brenda
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Q93RP5_THETH
592
0
66227
TrEMBL
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crystal structure solved by multiple isomorphous replacement
crystallized by the hanging-drop vapour-diffusion method using ethylene glycol as precipitant
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N79D
can use tRNAArg with G20 as substrate
N79E
can use tRNAArg with G20 or U20 as substrate
N79K
can use tRNAArg with G20 as substrate
N79Q
can use tRNAArg with G20 as substrate
N79R
can use tRNAArg with G20 or U20 as substrate
Y77F
slight effect on activity
K116G
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expression of the mutant in Escherichia coli
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purification of the wild type and K116G mutant, expressed in Escherichia coli, by DEAE-Sephacel and PhenylSuperose column chromatography
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overexpressed in Escherichia coli under the control of the T7 promoter
overexpression in Escherichia coli of wild type and several mutant enzymes
expression of the wild type and K116G mutant in Escherichia coli
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Shimada, A.; Nureki, O.; Dohmae, N.; Takio, K.; Yokoyama, S.
Gene cloning, expression, crystallization and preliminary X-ray analysis of Thermus thermophilus arginyl-tRNA synthetase
Acta Crystallogr. Sect. D
57
272-275
2001
Thermus thermophilus (Q93RP5), Thermus thermophilus
brenda
Sekine, S.; Shimada, A.; Nureki, O.; Cavarelli, J.; Moras, D.; Vassylyev, D.G.; Yokoyama, S.
Crucial role of the high-loop lysine for the catalytic activity of arginyl-tRNA synthetase
J. Biol. Chem.
276
3723-3726
2001
Thermus thermophilus
brenda
Shimada, A.; Nureki, O.; Goto, M.; Takahashi, S.; Yokoyama, S.
Structural and mutational studies of the recognition of the arginine tRNA-specific major identity element, A20, by arginyl-tRNA synthetase
Proc. Natl. Acad. Sci. USA
98
13537-13542
2001
Thermus thermophilus (Q93RP5), Thermus thermophilus
brenda