Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli of wild type and several mutant enzymes | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
crystal structure solved by multiple isomorphous replacement | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
N79D | can use tRNAArg with G20 as substrate | Thermus thermophilus |
N79E | can use tRNAArg with G20 or U20 as substrate | Thermus thermophilus |
N79K | can use tRNAArg with G20 as substrate | Thermus thermophilus |
N79Q | can use tRNAArg with G20 as substrate | Thermus thermophilus |
N79R | can use tRNAArg with G20 or U20 as substrate | Thermus thermophilus |
Y77A | inactive mutant | Thermus thermophilus |
Y77F | slight effect on activity | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values for the wild type and mutant enzymes using tRNAArg with different nucleotides at position 20 | Thermus thermophilus | |
0.0098 | - |
tRNAArg | adenine at position 20 of the tRNAArg is required for activity, pH 7.5, 65°C | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q93RP5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Thermus thermophilus | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |