EC Number   |
Protein Variants |
Reference |
|---|
    6.1.1.19 | argS MA5002 |
mutant argS MA5002 differs from the wild-type ArgRS structural gene by one mutation, a substitution of an Arg by a Ser at position 134. It exhibits a 4times to 6times as low activity and a 5times as high Km value for ATP as the wild-type enzyme in aminoacylation and ATP-diphosphate exchange, Km values for Arg and tRNAArg remain unaltered |
263 |
| 6.1.1.19 | C320A |
slight effect on activity |
649854 |
| 6.1.1.19 | C537A |
slight effect on activity |
649854 |
| 6.1.1.19 | C599Y |
expression in Saccharomices cerevisiae, shows reduced activity and stability |
652831 |
| 6.1.1.19 | D118A |
the mutation results in a 2fold increase in binding affinity |
728718 |
| 6.1.1.19 | D317A |
inactive |
728718 |
| 6.1.1.19 | DELTA1-73 |
KM-value for tRNAArg is 1.2fold higher than the wild-type value, turnover number is 1.4fold higher |
661127 |
| 6.1.1.19 | DELTANArgRS |
lacking the N-terminal side from the 91st residue |
703658 |
| 6.1.1.19 | DELTANhcArgRS |
N-terminal 72-amino acid deletion mutant with higher specific activity than the wild type enzyme |
672090 |
| 6.1.1.19 | G136K |
inactive enzyme |
649768 |
