Cloned (Comment) | Organism |
---|---|
expression of the wild type and K116G mutant in Escherichia coli | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
K116G | expression of the mutant in Escherichia coli | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of the wild type and K116G mutant, expressed in Escherichia coli, by DEAE-Sephacel and PhenylSuperose column chromatography | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
K116G mutant displays very low activity compared to wild type enzyme | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Thermus thermophilus | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |