Information on EC 2.8.3.1 - propionate CoA-transferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.8.3.1
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RECOMMENDED NAME
GeneOntology No.
propionate CoA-transferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + propanoate = acetate + propanoyl-CoA
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
coenzyme A transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1,2-propanediol biosynthesis from lactate (engineered)
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4-aminobutanoate degradation V
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acetyl-CoA fermentation to butanoate II
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fermentation to 2-methylbutanoate
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gallate degradation III (anaerobic)
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L-glutamate degradation V (via hydroxyglutarate)
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pyruvate fermentation to propanoate II (acrylate pathway)
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succinate fermentation to butanoate
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alanine metabolism
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CO2 fixation in Crenarchaeota
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propanol degradation
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Pyruvate metabolism
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Propanoate metabolism
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Styrene degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:propanoate CoA-transferase
Butanoate and lactate can also act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-15-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
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Manually annotated by BRENDA team
Propionibacterium freudenreichii subsp. shermanii
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Manually annotated by BRENDA team
Propionibacterium freudenreichii subsp. shermanii
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-lactate + propionyl-CoA
propionate + (R)-lactoyl-CoA
show the reaction diagram
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-
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r
3-hydroxybutyrate + acetyl-CoA
acetate + 3-hydroxybutanoyl-CoA
show the reaction diagram
3-hydroxybutyryl-CoA + propanoate
?
show the reaction diagram
-
highest rate of reaction with 3-hydroxybutyryl-CoA (100% activity)
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?
3-hydroxypropanoate + acetyl-CoA
acetate + 3-hydroxypropanoyl-CoA
show the reaction diagram
4-hydroxybutyrate + acetyl-CoA
acetate + 4-hydroxybutanoyl-CoA
show the reaction diagram
acetate + propanoyl-CoA
acetyl-CoA + propanoate
show the reaction diagram
acetyl-CoA + 2-hydroxybutanoate
acetate + 2-hydroxybutyryl-CoA
show the reaction diagram
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-
-
-
?
acetyl-CoA + 2-hydroxybutyrate
acetate + 2-hydroxybutyryl-CoA
show the reaction diagram
acetyl-CoA + 3-hydroxybutyrate
acetate + 3-hydroxybutyryl-CoA
show the reaction diagram
acetyl-CoA + 3-hydroxypropanoate
acetate + 3-hydroxypropanoyl-CoA
show the reaction diagram
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-
-
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?
acetyl-CoA + 3-hydroxypropionate
acetate + 3-hydroxypropionyl-CoA
show the reaction diagram
acetyl-CoA + 3-mercaptopropionate
acetate + 3-mercaptopropionyl-CoA
show the reaction diagram
acetyl-CoA + 4-hydroxybutyrate
acetate + 4-hydroxybutyryl-CoA
show the reaction diagram
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-
-
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?
acetyl-CoA + acetoacetate
acetate + acetoacetyl-CoA
show the reaction diagram
acetyl-CoA + acrylate
acetate + acryloyl-CoA
show the reaction diagram
acetyl-CoA + butanoate
acetate + butanoyl-CoA
show the reaction diagram
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-
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acetyl-CoA + butyrate
acetate + butanoyl-CoA
show the reaction diagram
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low activity
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?
acetyl-CoA + butyrate
acetate + butyryl-CoA
show the reaction diagram
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?
acetyl-CoA + chloropropionate
acetate + chloropropionyl-CoA
show the reaction diagram
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?
acetyl-CoA + crotonate
acetate + crotonoyl-CoA
show the reaction diagram
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?
acetyl-CoA + glycolate
acetate + glycolyl-CoA
show the reaction diagram
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no activity at 1 mM and 1% activity at 20 mM compared to propanoate
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?
acetyl-CoA + hexanoate
acetate + hexanoyl-CoA
show the reaction diagram
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4% activity at 1 mM and 21% activity at 20 mM compared to propanoate
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?
acetyl-CoA + isobutyrate
acetate + isobutyryl-CoA
show the reaction diagram
-
isobutyrate is preferentially used as CoA acceptor (34% activity at 1n mM and 100% activity at 20 mM compared to propanoate)
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?
acetyl-CoA + isovalerate
acetate + isovaleryl-CoA
show the reaction diagram
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2% activity at 1 mM and 6% activity at 20 mM compared to propanoate
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?
acetyl-CoA + lactate
acetate + lactoyl-CoA
show the reaction diagram
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?
acetyl-CoA + lactate
pyruvate + acetate + CoA
show the reaction diagram
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(R)-lactate preferred over (S)-lactate
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acetyl-CoA + octanoate
acetate + octanoyl-CoA
show the reaction diagram
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8% activity at 1 mM and 35% activity at 20 mM compared to propanoate
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?
acetyl-CoA + oxaloacetate
acetate + oxaloacetyl-CoA
show the reaction diagram
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acetyl-CoA + propanoate
acetate + propanoyl-CoA
show the reaction diagram
acetyl-CoA + propionate
acetate + propanoyl-CoA
show the reaction diagram
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?
acetyl-CoA + succinate
acetate + succinoyl-CoA
show the reaction diagram
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?
acetyl-CoA + trans-2,3-octenoate
acetate + trans-2,3-octenoyl-CoA
show the reaction diagram
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1% activity at 1 mM and 5% activity at 20 mM compared to propanoate
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?
acetyl-CoA + trans-2,3-pentenoate
acetate + trans-2,3-pentenoyl-CoA
show the reaction diagram
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5% activity at 1 mM and 34% activity at 20 mM compared to propanoate
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?
acetyl-CoA + valerate
acetate + valeryl-CoA
show the reaction diagram
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14% activity at 1 mM and 41% activity at 20 mM compared to propanoate
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?
acrylate + acrylyl-CoA
acetate + propionyl-CoA
show the reaction diagram
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69% activity compared to propionate
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?
butyrate + acetyl-CoA
acetate + butyryl-CoA
show the reaction diagram
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28% activity compared to propionate
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?
butyryl-CoA + propanoate
?
show the reaction diagram
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2% activity compared to 3-hydroxybutyryl-CoA
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?
crotonate + acetyl-CoA
acetate + crotonyl-CoA
show the reaction diagram
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28% activity compared to propionate
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?
crotonyl-CoA + propanoate
?
show the reaction diagram
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2% activity compared to 3-hydroxybutyryl-CoA
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?
isobutyryl-CoA + propanoate
?
show the reaction diagram
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6% activity compared to 3-hydroxybutyryl-CoA
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?
lactate + acetyl-CoA
acetate + lactoyl-CoA
show the reaction diagram
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36% activity compared to propionate
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?
propionate + acetyl-CoA
acetate + propionyl-CoA
show the reaction diagram
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propionate is the best substrate, 100% activity
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?
propionyl-CoA + acetate
propanoate + acetyl-CoA
show the reaction diagram
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?
propionyl-CoA + propanoate
?
show the reaction diagram
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27% activity compared to 3-hydroxybutyryl-CoA
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?
propionyl-CoA + succinate
propanoate + succinoyl-CoA
show the reaction diagram
succinate + acetyl-CoA
acetate + succinyl-CoA
show the reaction diagram
succinyl-CoA + propanoate
?
show the reaction diagram
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1% activity compared to 3-hydroxybutyryl-CoA
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?
valeryl-CoA + propanoate
?
show the reaction diagram
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3% activity compared to 3-hydroxybutyryl-CoA
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetate + propanoyl-CoA
acetyl-CoA + propanoate
show the reaction diagram
acetyl-CoA + propanoate
acetate + propanoyl-CoA
show the reaction diagram
acetyl-CoA + propionate
acetate + propanoyl-CoA
show the reaction diagram
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?
propionyl-CoA + succinate
propanoate + succinoyl-CoA
show the reaction diagram
additional information
?
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The wild-type Pct is not able to efficiently convert lactate to lactyl-CoA
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-lactate
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33% inhibition at 11 mM
(S)-lactate
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54% inhibition at 11 mM
Acrylate
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43% inhibition at 11 mM
Butyrate
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72% inhibition at 11 mM
hydroxylamine
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rapid and irreversible inactivation
propionate
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80% inhibition at 11 mM
Sodium borohydride
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rapid and irreversible inactivation
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
propionate
increase in propionate concentration lead to increase of enzyme amount
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.5
3-hydroxybutyrate
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at pH 7.4 and 30°C
4.5
acetate
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at pH 7.4 and 30°C
0.6
acetyl-CoA
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at pH 7.4 and 30°C
0.035 - 0.36
propionate
0.3
propionyl-CoA
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at pH 7.4 and 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14.2
propionate
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pH 7.0, 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.34
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substrate propionate
additional information
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development of propionate-detection systems, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
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over 90% of maximal activity within this range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 40
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over 90% of maximal activity within this range
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
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x * 56000, SDS-PAGE
57000
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4 * 57000, calculated from amino acid sequence
60000
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x * 60000, SDS-PAGE
64000
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4 * 64000, SDS-PAGE
67000
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4 * 67000, SDS-PAGE
224000
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gel filtration
228000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
tetramer
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4 * 67000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, for at least 1 month without loss of activity
4°C, in saturated ammonium sulfate, several months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni Sepharose column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
coexpression of the engineered mutant Pct540 from Clostridium propionicum in Escherichia coli strain XL-1 Blue with engineered Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate synthase 1 mutant, i.e. PhaC1Ps6-19. The expressed wild-type Pct is not able to efficiently convert lactate to lactyl-CoA and exerts an inhibitory effect on cell growth
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coexpression of the engineered mutant Pct540 from Clostridium propionicum in the engineered Escherichia coli strain JLX7 with engineered Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate synthase 1 mutant, i.e. PhaC1Ps6-19, production of polylactic acid, flux response analysis, overview. The engineered Escherichia coli strain exerts several knocked out genes, overview
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH5alpha cells
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expressed in Escherichia coli strains YH090 and YH091
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expressed in Escherichia coli XL1-Blue cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
V193A
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the Pct540 mutant contains the V193A mutation, and four silent nucleotide exchanges of T78C, T669C, A1125G, T1158C
additional information
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construction of an engineered enzyme mutant, Pct540, for the generation of poly(3-hydroxybutyrate-co-lactate) and lactyl-CoA endogenously, random mutagenesis, overview. Method optimization and molecular weight of synthesized polymers, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
amperometric propionate sensor
biotechnology
synthesis