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Literature summary for 2.8.3.1 extracted from

  • Yang, T.H.; Kim, T.W.; Kang, H.O.; Lee, S.H.; Lee, E.J.; Lim, S.C.; Oh, S.O.; Song, A.J.; Park, S.J.; Lee, S.Y.
    Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase (2010), Biotechnol. Bioeng., 105, 150-160.
    View publication on PubMed
Search for more literature for 2.8.3.1

Application

Application Comment Organism
synthesis engineered mutant Pct, coexpressed with an engineered Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate synthase 1, is able to produce poly(3-hydroxybutyrate-co-lactate) copolymers having 9-64 mol% of lactate. Polylactic acid might be a good alternative to petroleum-based plastic as it possesses several desirable properties such as biodegradability, biocompatibility, compostability, and low toxicity to humans Anaerotignum propionicum

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of the engineered mutant Pct540 from Clostridium propionicum in Escherichia coli strain XL-1 Blue with engineered Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate synthase 1 mutant, i.e. PhaC1Ps6-19. The expressed wild-type Pct is not able to efficiently convert lactate to lactyl-CoA and exerts an inhibitory effect on cell growth Anaerotignum propionicum

Protein Variants

Protein Variants Comment Organism
additional information construction of an engineered enzyme mutant, Pct540, for the generation of poly(3-hydroxybutyrate-co-lactate) and lactyl-CoA endogenously, random mutagenesis, overview. Method optimization and molecular weight of synthesized polymers, overview Anaerotignum propionicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Anaerotignum propionicum The wild-type Pct is not able to efficiently convert lactate to lactyl-CoA ?
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 ?

Organism

Organism UniProt Comment Textmining
Anaerotignum propionicum
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information The wild-type Pct is not able to efficiently convert lactate to lactyl-CoA Anaerotignum propionicum ?
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 ?

Synonyms

Synonyms Comment Organism
PCT
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 Anaerotignum propionicum
propionate CoA transferase
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 Anaerotignum propionicum