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Information on EC 2.7.11.1 - non-specific serine/threonine protein kinase and Organism(s) Saccharolobus solfataricus and UniProt Accession Q97ZY9
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2.7.11.1 non-specific serine/threonine protein kinaseIUBMB Comments
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
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Word Map
- 2.7.11.1
- jnk
- mapks
-
signal-regulated
- rapamycin
- agonist
- necrosis
- endothelial
- cardiac
- mtor
- parkinsonism
- caspase-3
- metastasis
- bcl-2
- synaptic
- cyclin
- alzheimer
- cyclase
- hippocampal
- forskolin
- sirnas
- neurodegenerative
- autophosphorylation
-
neuroprotective
- creb
-
phorbol
- tumorigenesis
- cardiomyocytes
- wortmannin
- pten
-
cyclin-dependent
- ventricular
- gtpases
- myocytes
- cytoskeleton
-
adenylyl
- nf-kappab
- myocardial
- ischemia
-
dominant-negative
- nmda
-
hyperphosphorylation
-
postsynaptic
-
anti-apoptotic
- src
- phosphoprotein
- isoproterenol
- rhoa
- staurosporine
-
tensin
-
l-type
- medicine
- pharmacology
- molecular biology
- drug development
The taxonomic range for the selected organisms is: Saccharolobus solfataricus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
protein kinase a, 14-3-3, camkii, p-akt, lrrk2, c-jun n-terminal kinase, serine/threonine kinase, raf-1, protein kinase b, gsk-3, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
A-kinase
-
-
-
-
A-T, mutated
-
-
-
-
A-T, mutated homolog
-
-
-
-
AMH type II receptor
-
-
-
-
Anti-sigma B factor rsbT
-
-
-
-
AP50 kinase
-
-
-
-
Ataxia telangiectasia mutated
-
-
-
-
Ataxia telangiectasia mutated homolog
-
-
-
-
ATP-protein transphosphorylase
-
-
-
-
betaIIPKC
-
-
-
-
Breast-tumor-amplified kinase
-
-
-
-
Calcium-dependent protein kinase C
-
-
-
-
Calcium/phospholipid-dependent protein kinase
-
-
-
-
cAMP-dependent protein kinase A
-
-
-
-
casein kinase
-
-
-
-
casein kinase 2
-
-
-
-
casein kinase I
-
-
-
-
casein kinase II
-
-
-
-
casein kinase-2
-
-
-
-
CDC25 suppressing protein kinase
-
-
-
-
Cell division cycle 2-like
-
-
-
-
CK-2
-
-
-
-
CK2
-
-
-
-
CK2-alpha
-
-
-
-
CKI
-
-
-
-
CKII
-
-
-
-
CLP-36 interacting kinase
-
-
-
-
cyclic monophosphate-dependent protein kinase
-
-
-
-
cyclic nucleotide-dependent protein kinase
-
-
-
-
cyclin-dependent kinase
-
-
-
-
cytidine 3',5'-cyclic monophosphate-responsive protein kinase
-
-
-
-
DNA-PKcs
-
-
-
-
DNPK1
-
-
-
-
dSTPK61
-
-
-
-
G11 protein
-
-
-
-
hARK1
-
-
-
-
histone kinase
-
-
-
-
hPDK1
-
-
-
-
Hpr kinase
-
-
-
-
hPSK
-
-
-
-
HSPK 21
-
-
-
-
HSPK 36
-
-
-
-
hydroxyalkyl-protein kinase
-
-
-
-
Kinase interacting with stathmin
-
-
-
-
kinase, casein (phosphorylating)
-
-
-
-
kinase, protamine (phosphorylating)
-
-
-
-
kinase, protein (phosphorylating)
-
-
-
-
kinase, protein, A (phosphorylating)
-
-
-
-
kinase, protein, C (phosphorylating)
-
-
-
-
Lymphocyte-oriented kinase
-
-
-
-
M phase-specific cdc2 kinase
-
-
-
-
mitogen-activated S6 kinase
-
-
-
-
mPDK1
-
-
-
-
MPSK
-
-
-
-
Myristoylated and palmitoylated serine-threonine kinase
-
-
-
-
NY-REN-55 antigen
-
-
-
-
P-CIP2
-
-
-
-
P460
-
-
-
-
p46Eg265
-
-
-
-
p46XlEg22
-
-
-
-
p82 kinase
-
-
-
-
pEg2
-
-
-
-
phosphorylase b kinase kinase
-
-
-
-
Phototropin
-
-
-
-
Pim-2h
-
-
-
-
PKA
-
-
-
-
PkB kinase
-
-
-
-
pp39-mos
-
-
-
-
Pre-mRNA protein kinase
-
-
-
-
protamine kinase
-
-
-
-
protein glutamyl kinase
-
-
-
-
protein kinase (phosphorylating)
-
-
-
-
Protein kinase B kinase
-
-
-
-
protein kinase CK2
-
-
-
-
Protein kinase Krct
-
-
-
-
Protein kinase MST
-
-
-
-
protein kinase p58
-
-
-
-
Protein kinase PKL12
-
-
-
-
protein phosphokinase
-
-
-
-
protein serine kinase
-
-
-
-
protein serine-threonine kinase
-
-
-
-
protein-aspartyl kinase
-
-
-
-
protein-cysteine kinase
-
-
-
-
protein-serine kinase
-
-
-
-
protein-serine/threonine kinase
-
-
-
-
PRP4 kinase
-
-
-
-
PRP4 pre-mRNA processing factor 4 homolog
-
-
-
-
PSK-H1
-
-
-
-
Raf kinase
-
-
-
-
Raf-1
-
-
-
-
ratAurA
-
-
-
-
ribosomal protein S6 kinase II
-
-
-
-
ribosomal S6 protein kinase
-
-
-
-
RP1 protein
-
-
-
-
S-receptor kinase
-
-
-
-
serine kinase
-
-
-
-
serine protein kinase
-
-
-
-
serine(threonine) protein kinase
-
-
-
-
serine-specific protein kinase
-
-
-
-
serine/threonine kinase
-
-
-
-
Serine/threonine kinase 15
-
-
-
-
Serine/threonine kinase Ayk1
-
-
-
-
serine/threonine protein kinase
-
-
-
-
Serine/threonine-protein kinase NRK2
-
-
-
-
Serine/threonine-protein kinase NYD-SPK
-
-
-
-
SRK
-
-
-
-
Ste20-like kinase
-
-
-
-
STE20-like kinase MST1
-
-
-
-
STE20-like kinase MST2
-
-
-
-
STE20-like kinase MST3
-
-
-
-
Switch protein/serine kinase
-
-
-
-
T-antigen kinase
-
-
-
-
threonine-specific protein kinase
-
-
-
-
Twitchin kinase
-
-
-
-
type-2 casein kinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (non-specific)
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
CAS REGISTRY NUMBER
COMMENTARY
191808-15-8
phosphoinositide dependent protein kinase 1
340830-03-7
-
37278-10-7
-
377752-08-4
ribosomal protein S6 kinase 2
389133-24-8
ribosomal S6 kinase 3
52660-18-1
casein kinase, protein kinase CK2
9026-43-1
this CAS Reg. No. encompasses a great variety of protein kinases including the serine/threonine specific kinases
90698-26-3
ribosomal protein S6 kinase 1
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
protein kinase activity, with a noticeable preference for phosphorylating proteins of acidic character. No activity with basic proteins such as histones or myelin basic protein. The protein kinase also can phosphorylate itself on serine and threonine residues. No autophosphorylation of the protein kinase can be detected prior to addition of exogenous substrate proteins. Thr-151 is a site of autophosphorylation, and that autophosphorylation of this residue enhances the catalytic efficiency of the enzyme
-
-
?
ATP + reduced carboxymethylated lysozyme
ADP + phopshorylated reduced carboxymethylated lysozyme
-
-
-
?
ADP + casein
AMP + phosphorylated casein
-
phosphorylated on threonine residues
-
-
?
ATP + bovine serum albumin
ADP + phosphorylated bovine serum albumin
-
-
-
-
?
ATP + casein
ADP + phosphorylated casein
-
phosphorylated on threonine residues
-
-
?
ATP + histone H4
ADP + phosphorylated histone H4
-
phosphorylated on threonine and on serine residues
-
-
?
ATP + KKRAARASSNVFA
ADP + ?
-
phosphorylation of the peptide is undetectable at 25°C. Phosphorylation takes place at measurable rates if the assay temperature is raised to 65°C or if poly(Glu4:Tyr) is included in the assay mixture
-
-
?
ATP + lysozyme
ADP + phopshorylated lysozyme
-
reduced carboxyamidomethylated and maleylated lysozyme
-
-
?
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
-
-
-
-
?
ATP + reduced carboxyamidomethylated and maleylated lysozyme
ADP + phosphorylated reduced carboxyamidomethylated and maleylated lysozyme
-
phosphorylated on threonine residues
-
-
?
GDP + casein
GMP + phosphorylated casein
-
phosphorylated on threonine residues
-
-
?
GTP + casein
GDP + phosphorylated casein
-
phosphorylated on threonine residues
-
-
?
GTP + partially dephosphorylated casein
GDP + ?
-
the enzyme catalyzes the transfer of phosphate from GTP at approximately two-thirds the rate observed with ATP
-
-
?
ATP + a protein
ADP + a phosphoprotein
-
phosphorylation of exogenous substrates takes place on serine and, occasionally, threonine, but the enzyme displays a strong preference for threonine as the phospho-acceptor amino acid residue, overview, no activity with GTP except for the autophosphorylation reaction in vitro which functions with GTP and also GDP
-
-
?
ATP + a protein
ADP + a phosphoprotein
-
PK2 phosphorylate itself as well as several exogenous proteins, including mixed histones, casein, bovine serum albumin, and reduced carboxyamidomethylated and maleylated lysozyme, on serine residues, overview, autophosphorylation of rSsoPK2 can be uncoupled from the phosphorylation of exogenous proteins by manipulation of the temperature or mutagenic alteration of the enzyme, autophosphorylation is detected only at temperatures above 60°C, whereas phosphorylation of exogenous proteins is detectable at 37°C
-
-
?
additional information
?
-
-
the enzyme performs autophosphorylation in vitro
-
-
?
additional information
?
-
-
neither of the pyrimidine nucleotide triphosphates tested, CTP and UTP, serves as substrates and neither does AMP, GMP, or pyrophosphate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
activity requires the presence of a divalent metal ion cofactor. Mn2+ is most effective at supporting protein kinase activity. Mg2+ is moderately effective, while Ca2+, Ni2+, and Zn2+ are ineffective as cofactors
Mn2+
activity requires the presence of a divalent metal ion cofactor. Mn2+ is most effective at supporting protein kinase activity. Mg2+ is moderately effective, while Ca2+, Ni2+, and Zn2+ are ineffective as cofactors
Mg2+
Mn2+
Mn2+
-
preferred metal ion cofactor, especially for the autophosphorylation reaction
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine
-
i.e. ML-9, 0.5-1.0 mM, 50% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5'-AMP
activity is increased several-fold upon preincubation with millimolar concentrations of 5'-AMP. Activation is enhanced by the presence of ATP
ADPribose
activity is increased several-fold upon preincubation with submicromolar concentrations of ADPribose. Activation is enhanced by the presence of ATP. ADP-ribose acts by evoking a conformational transition in the enzyme. Other mono- and di-nucleotides are ineffective
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
substrate specificity of the recombinant enzyme, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
62000
-
x * 67000, glycosylated enzyme, SDS-PAGE, x * 62000, deglycosylated enzyme, SDS-PAGE
67000
67000
-
x * 67000, glycosylated enzyme, SDS-PAGE, x * 62000, deglycosylated enzyme, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 67000, glycosylated enzyme, SDS-PAGE, x * 62000, deglycosylated enzyme, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
-
the glycosylated enzyme binds to Galanthus nivalis agglutinin
phosphoprotein
phosphoprotein
-
the enzyme performs autophosphorylation at Ser548, determination of sites of autophosphorylation by mass spectrometry in two areas, one immediately N terminal to the region corresponding to subdomain I of eukaryotic protein kinases, and the second N terminal to the presumed activation loop located between subdomains VII and VIII, autophosphorylation of rSsoPK2 can be uncoupled from the phosphorylation of exogenous proteins by manipulation of the temperature or mutagenic alteration of the enzyme, autophosphorylation is detected only at temperatures above 60°C, whereas phosphorylation of exogenous proteins is detectable at 37°C
phosphoprotein
-
the protein kinase undergoes autophosphorylation on threonine
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
T151A
activity with reduced carboxymethylated lysozyme is much lower than wild-type activity. The stoichiometry of autophosphorylation is roughly half that of the unmodified protein
T151D
activity with reduced carboxymethylated lysozyme is much lower than wild-type activity. The stoichiometry of autophosphorylation is roughly half that of the unmodified protein
D394A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-tpe enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme from membranes partially by detergent solubilization and anion exchange chromatography
-
native enzyme partially by detergent solublization from membranes and anion exchange chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ORF sso0469, amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli
-
orf sso2387, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) in inclusion bodies
-
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
solubilization from inclusion bodies after recombinant expression in Escherichia coli by treatment with 5 M urea
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lower, B.H.; Kennelly, P.J.
The membrane-associated protein-serine/threonine kinase from Sulfolobus solfataricus is a glycoprotein.
J. Bacteriol.
184
2614-2619
2002
Saccharolobus solfataricus
Lower, B.H.; Kennelly, P.J.
Open reading frame sso2387 from the archaeon Sulfolobus solfataricus encodes a polypeptide with protein-serine kinase activity
J. Bacteriol.
185
3436-3445
2003
Saccharolobus solfataricus
Lower, B.H.; Potters, M.B.; Kennelly, P.J.
A phosphoprotein from the archaeon Sulfolobus solfataricus with protein-serine/threonine kinase activity
J. Bacteriol.
186
463-472
2004
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Lower, B.H.; Bischoff, K.M.; Kennelly, P.J.
The archaeon Sulfolobus solfataricus contains a membrane-associated protein kinase activity that preferentially phosphorylates threonine residues in vitro
J. Bacteriol.
182
3452-3459
2000
Saccharolobus solfataricus
Bischoff, K.M.; Kennelly, P.J.
In-gel assay for identifying alternative nucleotide substrates for protein kinases
Anal. Biochem.
271
199-202
1999
Saccharolobus solfataricus
EXTERNAL LINKS (specific for EC-Number 2.7.11.1)
Transporter Classification Database (TCDB): 8.A.23.1.35, 9.B.321.1.1, 8.A.104.1.10, 1.A.87.2.4, 1.I.1.1.3, 1.A.87.2.9, 8.A.104.1.1, 1.A.87.2.6, 1.I.1.1.5, 9.A.15.1.1, 1.A.4.5.8, 1.A.4.5.1, 1.A.87.2.11, 8.A.104.1.9, 1.A.87.2.3, 8.A.104.1.5, 2.A.133.1.3
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