EC Number   |
|---|
   2.7.11.1 | - |
| 2.7.11.1 | an N-terminal His6 affinity tagged catalytic kinase domain construct of the alphaII isoform of S6K1 lacking the autoinhibitory domain (AID) (His6-S6K1alphaII(DELTA AID)-T389E), is expressed in Escherichia coli and purified by His6 affinity co-purification and by MonoQ anion exchange chromatography at pH 7.3 |
| 2.7.11.1 | cAMP-Sepharose affinity chromatography |
| 2.7.11.1 | cytosolic |
| 2.7.11.1 | FLAG immunoaffinity column chromatography |
| 2.7.11.1 | fusion protein of an amino-terminally truncated AK1 to the C-terminus of glutathione S-transferase |
| 2.7.11.1 | glutathione Sepharose column chromatography, and gel filtration |
| 2.7.11.1 | glutathione-immobilized bead chromatography, and gel filtration |
| 2.7.11.1 | glutathione-Sepharose bead chromatography |
| 2.7.11.1 | heparin-Sepharose column chromatography |
