Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine | i.e. ML-9, 0.5-1.0 mM, 50% inhibition | Saccharolobus solfataricus | |
1-(5-isoquinolinylsulfonyl)-2-methylpiperazine | i.e. H-7 | Saccharolobus solfataricus | |
genistein | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00074 | - |
ATP | pH 6.5, 25°C | Saccharolobus solfataricus | |
0.013 | - |
KKRAARATSNVFA | pH 6.5, 25°C, presence of poly(Glu4:Tyr) | Saccharolobus solfataricus | |
0.0157 | - |
ATP | pH 6.5, 65°C | Saccharolobus solfataricus | |
0.02 | - |
KKRAARASSNVFA | pH 6.5, 65°C | Saccharolobus solfataricus | |
0.03 | - |
KKRAARATSNVFA | pH 6.5, 25°C | Saccharolobus solfataricus | |
0.045 | - |
KKRAARATSNVFA | pH 6.5, 65°C | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Saccharolobus solfataricus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | potential divalent metal ion cofactors Mn2+ and Mg2+ | Saccharolobus solfataricus | |
Mn2+ | potential divalent metal ion cofactors Mn2+ and Mg2+ | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
2 * 67000, SDS-PAGE | Saccharolobus solfataricus |
125000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the protein kinase undergoes autophosphorylation on threonine | Saccharolobus solfataricus |
Purification (Comment) | Organism |
---|---|
partial | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0024 | - |
pH 6.5, 25°C, substrate: casein | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + casein | phosphorylated on threonine residues | Saccharolobus solfataricus | AMP + phosphorylated casein | - |
? | |
ATP + casein | phosphorylated on threonine residues | Saccharolobus solfataricus | ADP + phosphorylated casein | - |
? | |
ATP + histone H4 | phosphorylated on threonine and on serine residues | Saccharolobus solfataricus | ADP + phosphorylated histone H4 | - |
? | |
ATP + KKRAARASSNVFA | phosphorylation of the peptide is undetectable at 25°C. Phosphorylation takes place at measurable rates if the assay temperature is raised to 65°C or if poly(Glu4:Tyr) is included in the assay mixture | Saccharolobus solfataricus | ADP + ? | - |
? | |
ATP + KKRAARATSNVFA | - |
Saccharolobus solfataricus | ADP + KKRAARApTSNVFA | - |
? | |
ATP + reduced carboxyamidomethylated and maleylated lysozyme | phosphorylated on threonine residues | Saccharolobus solfataricus | ADP + phosphorylated reduced carboxyamidomethylated and maleylated lysozyme | - |
? | |
GDP + casein | phosphorylated on threonine residues | Saccharolobus solfataricus | GMP + phosphorylated casein | - |
? | |
GTP + casein | phosphorylated on threonine residues | Saccharolobus solfataricus | GDP + phosphorylated casein | - |
? | |
additional information | neither of the pyrimidine nucleotide triphosphates tested, CTP and UTP, serves as substrates and neither does AMP, GMP, or pyrophosphate | Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 67000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharolobus solfataricus |
65 | - |
assay at | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Saccharolobus solfataricus |