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Literature summary for 2.7.11.1 extracted from
- The membrane-associated protein-serine/threonine kinase from Sulfolobus solfataricus is a glycoprotein. (2002), J. Bacteriol., 184, 2614-2619.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | associated | Saccharolobus solfataricus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 67000, glycosylated enzyme, SDS-PAGE, x * 62000, deglycosylated enzyme, SDS-PAGE | Saccharolobus solfataricus |
| 67000 | - |
x * 67000, glycosylated enzyme, SDS-PAGE, x * 62000, deglycosylated enzyme, SDS-PAGE | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | Saccharolobus solfataricus | - |
ADP + a phosphoprotein | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the glycosylated enzyme binds to Galanthus nivalis agglutinin | Saccharolobus solfataricus |
| phosphoprotein | the enzyme performs autophosphorylation in vitro | Saccharolobus solfataricus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from membranes partially by detergent solubilization and anion exchange chromatography | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | - |
Saccharolobus solfataricus | ADP + a phosphoprotein | - |
? | |
| additional information | the enzyme performs autophosphorylation in vitro | Saccharolobus solfataricus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67000, glycosylated enzyme, SDS-PAGE, x * 62000, deglycosylated enzyme, SDS-PAGE | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| membrane-associated protein-serine/threonine kinase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
assay at | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Saccharolobus solfataricus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharolobus solfataricus | |
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