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Information on EC 2.7.11.1 - non-specific serine/threonine protein kinase and Organism(s) Oryza sativa and UniProt Accession P53682
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2.7.11.1 non-specific serine/threonine protein kinaseIUBMB Comments
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
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Word Map
- 2.7.11.1
- jnk
- mapks
-
signal-regulated
- rapamycin
- agonist
- necrosis
- endothelial
- cardiac
- mtor
- parkinsonism
- caspase-3
- metastasis
- bcl-2
- synaptic
- cyclin
- alzheimer
- cyclase
- hippocampal
- forskolin
- sirnas
- neurodegenerative
- autophosphorylation
-
neuroprotective
- creb
-
phorbol
- tumorigenesis
- cardiomyocytes
- wortmannin
- pten
-
cyclin-dependent
- ventricular
- gtpases
- myocytes
- cytoskeleton
-
adenylyl
- nf-kappab
- myocardial
- ischemia
-
dominant-negative
- nmda
-
hyperphosphorylation
-
postsynaptic
-
anti-apoptotic
- src
- phosphoprotein
- isoproterenol
- rhoa
- staurosporine
-
tensin
-
l-type
- medicine
- pharmacology
- molecular biology
- drug development
The taxonomic range for the selected organisms is: Oryza sativa
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
protein kinase a, 14-3-3, camkii, p-akt, lrrk2, c-jun n-terminal kinase, serine/threonine kinase, raf-1, protein kinase b, gsk-3, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
A-kinase
-
-
-
-
A-T, mutated
-
-
-
-
A-T, mutated homolog
-
-
-
-
AMH type II receptor
-
-
-
-
Anti-sigma B factor rsbT
-
-
-
-
AP50 kinase
-
-
-
-
Ataxia telangiectasia mutated
-
-
-
-
Ataxia telangiectasia mutated homolog
-
-
-
-
ATP-protein transphosphorylase
-
-
-
-
betaIIPKC
-
-
-
-
Breast-tumor-amplified kinase
-
-
-
-
Calcium-dependent protein kinase C
-
-
-
-
Calcium/phospholipid-dependent protein kinase
-
-
-
-
cAMP-dependent protein kinase A
-
-
-
-
casein kinase
-
-
-
-
casein kinase 2
-
-
-
-
casein kinase I
-
-
-
-
casein kinase II
casein kinase-2
-
-
-
-
CDC25 suppressing protein kinase
-
-
-
-
Cell division cycle 2-like
-
-
-
-
CK-2
-
-
-
-
CK2
-
-
-
-
CK2-alpha
-
-
-
-
CKI
-
-
-
-
CKII
CLP-36 interacting kinase
-
-
-
-
cyclic monophosphate-dependent protein kinase
-
-
-
-
cyclic nucleotide-dependent protein kinase
-
-
-
-
cyclin-dependent kinase
-
-
-
-
cytidine 3',5'-cyclic monophosphate-responsive protein kinase
-
-
-
-
DNA-PKcs
-
-
-
-
DNPK1
-
-
-
-
dSTPK61
-
-
-
-
G11 protein
-
-
-
-
hARK1
-
-
-
-
histone kinase
-
-
-
-
hPDK1
-
-
-
-
Hpr kinase
-
-
-
-
hPSK
-
-
-
-
HSPK 21
-
-
-
-
HSPK 36
-
-
-
-
hydroxyalkyl-protein kinase
-
-
-
-
Kinase interacting with stathmin
-
-
-
-
kinase, casein (phosphorylating)
-
-
-
-
kinase, protamine (phosphorylating)
-
-
-
-
kinase, protein (phosphorylating)
-
-
-
-
kinase, protein, A (phosphorylating)
-
-
-
-
kinase, protein, C (phosphorylating)
-
-
-
-
Lymphocyte-oriented kinase
-
-
-
-
M phase-specific cdc2 kinase
-
-
-
-
mitogen-activated S6 kinase
-
-
-
-
mPDK1
-
-
-
-
MPSK
-
-
-
-
Myristoylated and palmitoylated serine-threonine kinase
-
-
-
-
NY-REN-55 antigen
-
-
-
-
P-CIP2
-
-
-
-
P460
-
-
-
-
p46Eg265
-
-
-
-
p46XlEg22
-
-
-
-
p82 kinase
-
-
-
-
pEg2
-
-
-
-
phosphorylase b kinase kinase
-
-
-
-
Phototropin
-
-
-
-
Pim-2h
-
-
-
-
PKA
-
-
-
-
PkB kinase
-
-
-
-
pp39-mos
-
-
-
-
Pre-mRNA protein kinase
-
-
-
-
protamine kinase
-
-
-
-
protein glutamyl kinase
-
-
-
-
protein kinase (phosphorylating)
-
-
-
-
Protein kinase B kinase
-
-
-
-
protein kinase CK2
-
-
-
-
Protein kinase Krct
-
-
-
-
Protein kinase MST
-
-
-
-
protein kinase p58
-
-
-
-
Protein kinase PKL12
-
-
-
-
protein phosphokinase
-
-
-
-
protein serine kinase
-
-
-
-
protein serine-threonine kinase
-
-
-
-
protein-aspartyl kinase
-
-
-
-
protein-cysteine kinase
-
-
-
-
protein-serine kinase
-
-
-
-
protein-serine/threonine kinase
-
-
-
-
PRP4 kinase
-
-
-
-
PRP4 pre-mRNA processing factor 4 homolog
-
-
-
-
PSK-H1
-
-
-
-
Raf kinase
-
-
-
-
Raf-1
-
-
-
-
ratAurA
-
-
-
-
ribosomal protein S6 kinase II
-
-
-
-
ribosomal S6 protein kinase
-
-
-
-
RP1 protein
-
-
-
-
S-receptor kinase
-
-
-
-
serine kinase
-
-
-
-
serine protein kinase
-
-
-
-
serine(threonine) protein kinase
-
-
-
-
serine-specific protein kinase
-
-
-
-
serine/threonine kinase
-
-
-
-
Serine/threonine kinase 15
-
-
-
-
Serine/threonine kinase Ayk1
-
-
-
-
serine/threonine protein kinase
Serine/threonine-protein kinase NRK2
-
-
-
-
Serine/threonine-protein kinase NYD-SPK
-
-
-
-
SRK
-
-
-
-
Ste20-like kinase
-
-
-
-
STE20-like kinase MST1
-
-
-
-
STE20-like kinase MST2
-
-
-
-
STE20-like kinase MST3
-
-
-
-
Switch protein/serine kinase
-
-
-
-
T-antigen kinase
-
-
-
-
threonine-specific protein kinase
-
-
-
-
Twitchin kinase
-
-
-
-
type-2 casein kinase
-
-
-
-
casein kinase II
-
-
-
-
CKII
-
-
-
-
serine/threonine protein kinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (non-specific)
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
CAS REGISTRY NUMBER
COMMENTARY
191808-15-8
phosphoinositide dependent protein kinase 1
340830-03-7
-
37278-10-7
-
377752-08-4
ribosomal protein S6 kinase 2
389133-24-8
ribosomal S6 kinase 3
52660-18-1
casein kinase, protein kinase CK2
9026-43-1
this CAS Reg. No. encompasses a great variety of protein kinases including the serine/threonine specific kinases
90698-26-3
ribosomal protein S6 kinase 1
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + RNP29
ADP + phospho-RNP29
recombinant Strep-tagged substrate expressed from Escherichia coli, phosphorylation of the canonical pCKII motif ESEGE in the model substrate, identification of the RNP29 phosphorylation site by peptide mapping and mass spectrometry
-
-
?
GTP + RNP29
GDP + phospho-RNP29
recombinant Strep-tagged substrate expressed from Escherichia coli, phosphorylation of the canonical pCKII motif ESEGE in the model substrate, identification of the RNP29 phosphorylation site by peptide mapping and mass spectrometry
-
-
?
additional information
?
-
enzyme is involved in the synthesis of seed storage compounds during seed development
-
-
?
additional information
?
-
-
enzyme is involved in the synthesis of seed storage compounds during seed development
-
-
?
additional information
?
-
rice leaf phosphoproteome analysis, overview
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
enzyme is involved in the synthesis of seed storage compounds during seed development
-
-
?
additional information
?
-
-
enzyme is involved in the synthesis of seed storage compounds during seed development
-
-
?
additional information
?
-
rice leaf phosphoproteome analysis, overview
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
additional information
additional information
the phosphorylation activity is dependent on the presence of divalent ions such as Mg2+ or Mn2+
additional information
-
the phosphorylation activity is dependent on the presence of divalent ions such as Mg2+ or Mn2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
polylysine
poly-lysine has a substrate-specific inhibitory effect on the CKII alpha subunit, whereas it stimulates the activity of the CKII holoenzyme. Polylysine inhibits RNP29 phosphorylation by CKIIalpha
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
polylysine
poly-lysine has a substrate-specific inhibitory effect on the CKII alpha subunit, whereas it stimulates the activity of the CKII holoenzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
transient expression of GFP fused to the 184 N-terminal amino acids of the OspCKII sequence in rice confirmed the chloroplastic localization of the kinase, subcellular localization analysis. Plastid CKII has a very low abundance. The N-terminal extension of LOC_Os03g55490 functions as a plastid transit peptide in vivo
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
several pCKII phosphorylation sites in dicotyledonous plants are not conserved in monocots and algae, suggesting that details of pCKII regulation in plastids have changed during evolution
physiological function
physiological function
-
the enzyme is involved in antiviral defense signaling pathways in rice
physiological function
the plastid casein kinase II is part of the plastid RNA polymerase multisubunit enzyme complex as one of the associated regulatory factors that integrate the transcription machinery into different chloroplast processes. pCKII mediates transcriptional regulation by phosphorylation of RNA polymerase/TAC subunits
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
chloroplast CKIIs lack the alpha'- and the beta-subunits ad are formed by alpha-subunits only. Oryza sativa contains four CKII alpha subunits with high sequence identity, peptide mapping and mass spectrometry, overview
additional information
-
chloroplast CKIIs lack the alpha'- and the beta-subunits ad are formed by alpha-subunits only. Oryza sativa contains four CKII alpha subunits with high sequence identity, peptide mapping and mass spectrometry, overview
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
transgenic rice plants are generated with over-expression of SAPK4 under control of the CaMV-35S promoter. Induced expression of SAPK4 results in improved germination, growth and development under salt stress both in seedlings and mature plants. In response to salt stress, the transgenic rice accumulate less Na+ and Cl- and show improved photosynthesis. SAPK4-regulated genes with functions in ion homeostasis and oxidative stress response are identified: the vacuolar H+-ATPase, the Na+/H+ antiporter NHX1, the Cl- channel OsCLC1 and a catalase
additional information
-
transgenic rice plants are generated with over-expression of SAPK4 under control of the CaMV-35S promoter. Induced expression of SAPK4 results in improved germination, growth and development under salt stress both in seedlings and mature plants. In response to salt stress, the transgenic rice accumulate less Na+ and Cl- and show improved photosynthesis. SAPK4-regulated genes with functions in ion homeostasis and oxidative stress response are identified: the vacuolar H+-ATPase, the Na+/H+ antiporter NHX1, the Cl- channel OsCLC1 and a catalase
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
protoplast and chloroplast preparation from rice seedlings, purification of the enzyme from chloroplasts by heparin affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
loc os03g55490, DNA and amino acid sequence determination and analysis, sequence comparisons, transient expression of GFP fused to the 184 N-terminal amino acids of the OspCKII sequence in Oryza sativa, recombinant expression of CKIIalpha without chloroplast transit peptide in Escherichia coli strain BL21
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
molecular biology
SAPK4 regulates ion homeostasis and growth and development under salinity and this indicates a function of SAPK4 as a regulatory factor in plant salt stress acclimation
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kawasaki, T.; Hayashida, N.; Baba, T.; Shinozaki, K.; Shimada, H.
The gene encoding a calcium-dependent protein kinase located near the sbe1 gene encoding starch branching enzyme I is specifically expressed in developing rice seeds
Gene
129
183-189
1993
Oryza sativa (P53682), Oryza sativa
Breviario, D.; Morello, L.; Giani, S.
Molecular cloning of two novel rice cDNA sequences encoding putative calcium-dependent protein kinases
Plant Mol. Biol.
27
953-967
1995
Oryza sativa (P53683), Oryza sativa (P53684), Oryza sativa
Diedhiou, C.J.; Popova, O.V.; Dietz, K.J.; Golldack, D.
The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice
BMC Plant Biol.
8
49
2008
Oryza sativa (Q5N942), Oryza sativa
Lu, Q.; Ding, S.; Reiland, S.; Roediger, A.; Roschitzki, B.; Xue, P.; Gruissem, W.; Lu, C.; Baginsky, S.
Identification and characterization of chloroplast casein kinase II from Oryza sativa (rice)
J. Exp. Bot.
66
175-187
2015
Oryza sativa
Lee, K.; Kim, K.
The rice serine/threonine protein kinase OsPBL1 (Oryza sativa Arabidopsis pbs1-like 1) is potentially involved in resistance to rice stripe disease
Plant Growth Regul.
77
67-75
2015
Oryza sativa
-
EXTERNAL LINKS (specific for EC-Number 2.7.11.1)
Transporter Classification Database (TCDB): 8.A.23.1.35, 9.B.321.1.1, 8.A.104.1.10, 1.A.87.2.4, 1.I.1.1.3, 1.A.87.2.9, 8.A.104.1.1, 1.A.87.2.6, 1.I.1.1.5, 9.A.15.1.1, 1.A.4.5.8, 1.A.4.5.1, 1.A.87.2.11, 8.A.104.1.9, 1.A.87.2.3, 8.A.104.1.5, 2.A.133.1.3
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