This enzyme can also phosphorylate PtdIns3P in the 4-position, and PtdIns, PtdIns3P and PtdIns(3,4)P2 in the 5-position in vitro, but to a lesser extent. The last of these reactions occurs in vivo and is physiologically relevant. Three different isoforms are known.
Arabidopsis genome contains 11 genes encoding proteins with significant sequence similarity to animal PIP5Ks. These are classified into two subfamilies: subfamily A proteins (PIP5K10 and PIP5K11) consist of dimerization and catalytic domains similar to those in the animal enzymes, while subfamily B proteins (PIP5K1 to PIP5K9) have an additional domain containing a repeat of the membrane occupation and recognition nexus motif at the N-terminus.
This enzyme can also phosphorylate PtdIns3P in the 4-position, and PtdIns, PtdIns3P and PtdIns(3,4)P2 in the 5-position in vitro, but to a lesser extent. The last of these reactions occurs in vivo and is physiologically relevant. Three different isoforms are known.
PIP5K3 is involved in localizing 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate to the elongating root hair apex and is a key regulator of the machinery that initiates and promotes root hair tip growth.
GST-PIP5K3 produces 1-phosphatidyl-1D-myo-inositol 4,5 by phosphorylating 1-phosphatidyl-1D-myo-inositol 4-phosphate, both the natural and synthetic forms. Its also phosphorylates 1-phosphatidyl-1D-myo-inositol 5-phosphate, but less efficiently. PIP5K3 catalyzes the synthesis of 1-phosphatidyl-1D-myo-inositol 4,5 preferentially from 1-phosphatidyl-1D-myo-inositol 4-phosphate in vitro.
GST-PIP5K3 produces 1-phosphatidyl-1D-myo-inositol 4,5 by phosphorylating 1-phosphatidyl-1D-myo-inositol 4-phosphate, both the natural and synthetic forms. Its also phosphorylates 1-phosphatidyl-1D-myo-inositol 5-phosphate, but less efficiently. PIP5K3 catalyzes the synthesis of 1-phosphatidyl-1D-myo-inositol 4,5 preferentially from 1-phosphatidyl-1D-myo-inositol 4-phosphate in vitro.
PIP5K3 may function as a downstream effector of rho-related GTPases of plants (ROPs) in regulatory mechanisms, not only for the planar polarity of bulge initiation but also for the promotion of root hair tip growth.
PIP5K3 may function as a downstream effector of rho-related GTPases of plants (ROPs) in regulatory mechanisms, not only for the planar polarity of bulge initiation but also for the promotion of root hair tip growth.
Reduced expression of functional PIP5K3 is responsible for the short-root hair phenotype. Inducible overexpression of PIP5K3 enhanced root hair elongation and increased the rate of 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate
Reduced expression of functional PIP5K3 is responsible for the short-root hair phenotype. Inducible overexpression of PIP5K3 enhanced root hair elongation and increased the rate of 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate
PIP5K3 is involved in localizing 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate to the elongating root hair apex and is a key regulator of the machinery that initiates and promotes root hair tip growth.
GST-PIP5K3 produces 1-phosphatidyl-1D-myo-inositol 4,5 by phosphorylating 1-phosphatidyl-1D-myo-inositol 4-phosphate, both the natural and synthetic forms. Its also phosphorylates 1-phosphatidyl-1D-myo-inositol 5-phosphate, but less efficiently. PIP5K3 catalyzes the synthesis of 1-phosphatidyl-1D-myo-inositol 4,5 preferentially from 1-phosphatidyl-1D-myo-inositol 4-phosphate in vitro.
GST-PIP5K3 produces 1-phosphatidyl-1D-myo-inositol 4,5 by phosphorylating 1-phosphatidyl-1D-myo-inositol 4-phosphate, both the natural and synthetic forms. Its also phosphorylates 1-phosphatidyl-1D-myo-inositol 5-phosphate, but less efficiently. PIP5K3 catalyzes the synthesis of 1-phosphatidyl-1D-myo-inositol 4,5 preferentially from 1-phosphatidyl-1D-myo-inositol 4-phosphate in vitro.
PIP5K3 may function as a downstream effector of rho-related GTPases of plants (ROPs) in regulatory mechanisms, not only for the planar polarity of bulge initiation but also for the promotion of root hair tip growth.
PIP5K3 may function as a downstream effector of rho-related GTPases of plants (ROPs) in regulatory mechanisms, not only for the planar polarity of bulge initiation but also for the promotion of root hair tip growth.
Reduced expression of functional PIP5K3 is responsible for the short-root hair phenotype. Inducible overexpression of PIP5K3 enhanced root hair elongation and increased the rate of 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate
Reduced expression of functional PIP5K3 is responsible for the short-root hair phenotype. Inducible overexpression of PIP5K3 enhanced root hair elongation and increased the rate of 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate
t-DNA insertion mutations that reduces the expression of PIP5K3 cause shorter root hairs than in the wild type. Overexpression causes longer root hairs and multiple protruding sites on a single trichoblast.
phosphatidylinositol 4-phosphate 5-kinases 1 and 2 are involved in the regulation of vacuole morphology during Arabidopsis thaliana pollen development. Both isoforms are involved in protein trafficking to the vacuole in root tip epidermal cells
mutant plants homozygous for T-DNA-disrupted PIP5K3 alleles are compromised in root hair formation. Root hair-specific PIP5K3 overexpression results in root hair deformation and loss of cell polarity with increasing accumulation of PIP5K3 transcript. An N-terminal domain containing membrane occupation and recognition nexus repeats, which is not required for catalytic activity, is essential for the establishment of root hair growth. Transient heterologous expression of full-length PIP5K3 in Nicotiana tabacum pollen tubes increases plasma membrane association of a phosphatidylinositol 4,5-bisphoshate-specific reporter
Partial purification from Escherichia coli culture cells. The cells are suspended in PBS buffer, sonicated on ice, and centrifuged. Supernatant is mixed with glutathione-Sepharose 4B and incubated at 48°C for 2 h. The Sepharose beads are collected and washed with an excess volume of PBS.
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of enzyme form 10 as GST-fusion protein in Escherichia coli, expression of His-tagged enzyme form 10 in Spodoptera frugiperda Sf9 cells via baculovirus infection system
a yellow fluorescent (YFP) und GUS fusion PIP5K3 protein is expressed in Escherichia coli cells and transformed in Arabidopsis thaliana to produce trangenic plants. Overexpression and mutant is investigated.
expression of enzyme form 1 as GST-fusion protein in Escherichia coli, expression of His-tagged enzyme form 1 in Spodoptera frugiperda Sf9 cells via baculovirus infection system
Characterization and comparative analysis of Arabidopsis phosphatidylinositol phosphate 5-kinase 10 reveals differences in Arabidopsis and human phosphatidylinositol phosphate kinases
FEBS Lett.
579
3427-3432
2005
Arabidopsis thaliana (Q56YP2), Arabidopsis thaliana (Q9LMN1), Homo sapiens (Q99755), Homo sapiens
PIP5K9, an Arabidopsis phosphatidylinositol monophosphate kinase, interacts with a cytosolic invertase to negatively regulate sugar-mediated root growth