EC Number |
---|
2.7.1.68 | - |
2.7.1.68 | amylose-resin column chromatography |
2.7.1.68 | cytosolic enzyme type II enzyme and membrane-bound type I and type II enzyme |
2.7.1.68 | glutathione-Sepharose chromatography |
2.7.1.68 | glutathione-Sepharose column chromatography |
2.7.1.68 | Ni-NTA agarose column chromatography |
2.7.1.68 | Ni-NTA agarose column chromatography or glutathione-Sepharose column chromatography |
2.7.1.68 | partial |
2.7.1.68 | Partial purification from Escherichia coli culture cells. The cells are suspended in PBS buffer, sonicated on ice, and centrifuged. Supernatant is mixed with glutathione-Sepharose 4B and incubated at 48°C for 2 h. The Sepharose beads are collected and washed with an excess volume of PBS. |
2.7.1.68 | recombinan GST-fusion isozyme alpha from Escherichia coli |