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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68-
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68amylose-resin column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68cytosolic enzyme type II enzyme and membrane-bound type I and type II enzyme
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68glutathione-Sepharose chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68glutathione-Sepharose column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68Ni-NTA agarose column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68Ni-NTA agarose column chromatography or glutathione-Sepharose column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68partial
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68Partial purification from Escherichia coli culture cells. The cells are suspended in PBS buffer, sonicated on ice, and centrifuged. Supernatant is mixed with glutathione-Sepharose 4B and incubated at 48°C for 2 h. The Sepharose beads are collected and washed with an excess volume of PBS.
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68recombinan GST-fusion isozyme alpha from Escherichia coli
Results 1 - 10 of 13 > >>