Information on EC 2.4.1.281 - 4-O-beta-D-mannosyl-D-glucose phosphorylase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.4.1.281
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RECOMMENDED NAME
GeneOntology No.
4-O-beta-D-mannosyl-D-glucose phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate = D-glucose + alpha-D-mannose 1-phosphate
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta-(1,4)-mannan degradation
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SYSTEMATIC NAME
IUBMB Comments
4-O-beta-D-mannopyranosyl-D-glucopyranose:phosphate alpha-D-mannosyltransferase
This enzyme forms part of a mannan catabolic pathway in the anaerobic bacterium Bacteroides fragilis NCTC 9343.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,5-anhydro-D-glucitol + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-1,5-anhydro-D-glucitol + phosphate
show the reaction diagram
1,5-anhydro-D-glucitol + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
r
2-deoxy-2-fluoro-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-2-deoxy-2-fluoro-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
2-deoxy-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-2-deoxy-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
3-deoxy-3-fluoro-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-3-deoxy-3-fluoro-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
3-O-methyl-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-3-O-methyl-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
show the reaction diagram
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
show the reaction diagram
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
show the reaction diagram
cellobiose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
r
D-allose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-allose + phosphate
show the reaction diagram
-
-
-
r
D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
D-mannose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-mannopyranose + phosphate
show the reaction diagram
-
-
-
r
D-xylose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-xylopyranose + phosphate
show the reaction diagram
-
-
-
-
r
gentiobiose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
r
laminaribiose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
maltose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
r
methyl alpha-D-glucoside + alpha-D-mannose 1-phosphate
methyl [4-O-beta-D-mannopyranosyl]-alpha-D-glucoside + phosphate
show the reaction diagram
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-
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r
methyl beta-D-glucoside + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-methyl-beta-D-glucoside + phosphate
show the reaction diagram
-
-
-
-
r
methyl beta-D-glucoside + alpha-D-mannose 1-phosphate
methyl [4-O-beta-D-mannopyranosyl]-beta-D-glucopyranoside + phosphate
show the reaction diagram
-
-
-
r
N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
show the reaction diagram
-
-
-
r
sophorose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
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-
-
r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
show the reaction diagram
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
show the reaction diagram
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
show the reaction diagram
D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
laminaribiose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-D-mannose 1-phosphate
competitive; competitive
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
79.2
1,5-anhydro-D-glucitol
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
48.7
2-deoxy-2-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
90.7
2-deoxy-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
6.44
3-deoxy-3-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
7.82
3-O-methyl-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.304 - 27.7
4-O-beta-D-mannopyranosyl-D-glucopyranose
156
6-deoxy-6-fluoro-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
119
6-deoxy-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.324 - 36.5
alpha-D-mannose 1-phosphate
22.2
cellobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
6.14
D-Allose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
25.9 - 319
D-glucose
45.6
D-mannose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
60.8
gentiobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
29.1
laminaribiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
50.4
maltose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
85.3
methyl alpha-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
85.2
methyl beta-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
49.9
N,N'-diacetylchitobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
107
N-acetyl-D-glucosamine
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.444 - 5.34
phosphate
49.8
sophorose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20.7
1,5-anhydro-D-glucitol
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
5.71
2-deoxy-2-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
11.7
2-deoxy-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.94
3-deoxy-3-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.763
3-O-methyl-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
17.5 - 83.3
4-O-beta-D-mannopyranosyl-D-glucopyranose
42
6-deoxy-6-fluoro-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
177
6-deoxy-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
2.76 - 52.1
alpha-D-mannose 1-phosphate
20.2
cellobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.667
D-Allose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
19.3 - 126
D-glucose
5.12
D-mannose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
26.1
gentiobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
11.6
laminaribiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
5.07
maltose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
20.7
methyl alpha-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
35.2
methyl beta-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
6.34
N,N'-diacetylchitobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
15.8
N-acetyl-D-glucosamine
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
24.8
phosphate
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pH 7.5, 30°C
13.8
sophorose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.261
1,5-anhydro-D-glucitol
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.117
2-deoxy-2-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.129
2-deoxy-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.146
3-deoxy-3-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.0976
3-O-methyl-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
5.1
4-O-beta-D-mannopyranosyl-D-glucopyranose
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pH 7.5, 30°C
0.269
6-deoxy-6-fluoro-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
1.49
6-deoxy-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.076 - 21.6
alpha-D-mannose 1-phosphate
0.91
cellobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.109
D-Allose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.061 - 4.86
D-glucose
0.112
D-mannose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.429
gentiobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.399
laminaribiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.101
maltose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.236
methyl alpha-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.413
methyl beta-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.127
N,N'-diacetylchitobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.148
N-acetyl-D-glucosamine
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
12.4
phosphate
-
pH 7.5, 30°C
0.277
sophorose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28.5
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pH 7.5, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Ruminococcus albus (strain ATCC 27210 / DSM 20455 / JCM 14654 / NCDO 2250 / 7)
Ruminococcus albus (strain ATCC 27210 / DSM 20455 / JCM 14654 / NCDO 2250 / 7)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37823
6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence
38000
6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence
43646
2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence
43890
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calculated from amino acid sequence
80000
gel filtration
209000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
homohexamer
multimer
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x * 45000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to 1.68 A resolution. The enzyme shows a homohexameric structure, which is formed by using two helices attached to the N-terminus and C-terminus as a tab for sticking between subunits. In complexes with substrates, 4-O-beta-D-mannosyl-D-glucose and phosphate, and the product D-mannose-1-phosphate the structures reveal that the invariant residue Asp131, is supposed to be the general acid/base, does not exist close to the glycosidic Glc-O4 atom, which should be protonated in the catalytic reaction
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crystal structure with/without 4-O-beta-D-mannosyl-D-glucose. His245 of loop 3 forms a hydrogen-bond network with the substrate through a water molecule, and is indispensible for substrate binding
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 9.5
4°C, 24 h stable
725502
4.5 - 10.5
4°C, 24 h stable
725502
5.5 - 8.3
-
-
735796
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
stable up to
45
stable up to
80
-
stable below
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21; expressed in Escherichia coli BL21
expression in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is increased in presence of Konjac glucomannan
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Show AA Sequence (838 entries)
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