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Information on EC 2.3.1.224 - acetyl-CoA-benzylalcohol acetyltransferase and Organism(s) Clarkia breweri and UniProt Accession O64988

for references in articles please use BRENDA:EC2.3.1.224
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EC Tree
IUBMB Comments
The enzyme is found in flowers like Clarkia breweri, where it is important for floral scent production. Unlike EC 2.3.1.84, alcohol O-acetyltransferase, this enzyme is active with alcohols that contain a benzyl ring.
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This record set is specific for:
Clarkia breweri
UNIPROT: O64988
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Word Map
  • 2.3.1.224
  • arterial
  • stroke
  • haemodynamic
  • pacing
  • inotropic
  • wedge
  • scent
  • pressure-volume
  • mmhg-1
  • supine
  • vo2-pva
  • end-systolic
The taxonomic range for the selected organisms is: Clarkia breweri
The enzyme appears in selected viruses and cellular organisms
Synonyms
beat-1, acetyl-coa:benzylalcohol acetyltransferase, beat-3, benzylalcohol acetyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetyl-CoA:benzylalcohol acetyltransferase
-
-
BEAT-1
-
isoform
BEAT-2
-
isoform
benzylalcohol acetyltransferase
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + benzyl alcohol = CoA + benzyl acetate
show the reaction diagram
(1)
acetyl-CoA + cinnamyl alcohol = CoA + cinnamyl acetate
show the reaction diagram
(2)
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:benzylalcohol O-acetyltransferase
The enzyme is found in flowers like Clarkia breweri, where it is important for floral scent production. Unlike EC 2.3.1.84, alcohol O-acetyltransferase, this enzyme is active with alcohols that contain a benzyl ring.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + 2-(naphthalen-2-yl)ethanol
CoA + 2-(naphthalen-2-yl)ethyl acetate
show the reaction diagram
at 9% of the activity compared to benzyl alcohol, plant purified enzyme, at 28% of the activity compared to benzyl alcohol (Escherichia coli-expressed enzyme)
-
-
?
acetyl-CoA + 2-phenylethanol
CoA + 2-phenylethylacetate
show the reaction diagram
at 33% of the activity compared to benzyl alcohol, plant purified enzyme, at 57% of the activity compared to benzyl alcohol (Escherichia coli-expressed enzyme)
-
-
?
acetyl-CoA + benzyl alcohol
CoA + benzyl acetate
show the reaction diagram
-
-
-
?
acetyl-CoA + cinnamyl alcohol
CoA + cinnamyl acetate
show the reaction diagram
acetyl-CoA + 2-phenylethanol
CoA + 2-phenylethyl acetate
show the reaction diagram
-
28% activity compared to benzyl alcohol with isoform BEAT-1, 40% activity compared to benzyl alcohol with isoform BEAT-2
-
-
?
acetyl-CoA + 3-cis-hexene-1-ol
CoA + (3Z)-hex-3-en-1-yl acetate
show the reaction diagram
-
102% activity compared to benzyl alcohol with isoform BEAT-1, 102% activity compared to benzyl alcohol with isoform BEAT-2
-
-
?
acetyl-CoA + benzyl alcohol
CoA + benzyl acetate
show the reaction diagram
-
100% activity
-
-
?
acetyl-CoA + cinnamyl alcohol
CoA + cinnamyl acetate
show the reaction diagram
-
97% activity compared to benzyl alcohol with isoform BEAT-1, 122% activity compared to benzyl alcohol with isoform BEAT-2
-
-
?
acetyl-CoA + heptanol
CoA + heptyl acetate
show the reaction diagram
-
143% activity compared to benzyl alcohol with isoform BEAT-1, 150% activity compared to benzyl alcohol with isoform BEAT-2
-
-
?
acetyl-CoA + naphthalene ethanol
?
show the reaction diagram
-
57% activity compared to benzyl alcohol with isoform BEAT-1, 50% activity compared to benzyl alcohol with isoform BEAT-2
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + benzyl alcohol
CoA + benzyl acetate
show the reaction diagram
-
-
-
?
acetyl-CoA + cinnamyl alcohol
CoA + cinnamyl acetate
show the reaction diagram
-
-
-
?
acetyl-CoA + benzyl alcohol
CoA + benzyl acetate
show the reaction diagram
-
100% activity
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.024
acetyl-CoA
-
in 250 mM Tris-HCl pH 7.5, 25 mM MgCl2, 25 mM KC1, 10 mM 2-mercaptoethanol, at 30°C
0.12 - 0.24
benzyl alcohol
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
petal contain the majority of BEAT transcripts
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme is important for floral scent production
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BEATH_CLABR
433
0
48198
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48200
x * 48200, calculated from sequence
58000
1 * 58000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expressed in Escherichia coli BL21 cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the levels of BEAT mRNA in the petals increases as the bud matures, and peaks at anthesis
three days after anthesis, mRNA levels steeply declines
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dudareva, N.; D'Auria, J.C.; Nam, K.H.; Raguso, R.A.; Pichersky, E.
Acetyl-CoA:benzylalcohol acetyltransferase - an enzyme involved in floral scent production in Clarkia breweri
Plant J.
14
297-304
1998
Clarkia breweri (O64988), Clarkia breweri
Manually annotated by BRENDA team
Nam, K.H.; Dudareva, N.; Pichersky, E.
Characterization of benzylalcohol acetyltransferases in scented and non-scented Clarkia species
Plant Cell Physiol.
40
916-923
1999
Clarkia breweri, Clarkia concinna
Manually annotated by BRENDA team