Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Clarkia breweri |
expressed in Escherichia coli BL21 cells | Clarkia concinna |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
acetyl-CoA | in 250 mM Tris-HCl pH 7.5, 25 mM MgCl2, 25 mM KC1, 10 mM 2-mercaptoethanol, at 30°C | Clarkia breweri | |
0.12 | - |
benzyl alcohol | isoform BEAT-1, in 250 mM Tris-HCl pH 7.5, 25 mM MgCl2, 25 mM KC1, 10 mM 2-mercaptoethanol, at 30°C | Clarkia concinna | |
0.12 | - |
benzyl alcohol | isoform BEAT-2, in 250 mM Tris-HCl pH 7.5, 25 mM MgCl2, 25 mM KC1, 10 mM 2-mercaptoethanol, at 30°C | Clarkia breweri | |
0.24 | - |
benzyl alcohol | isoform BEAT-1, in 250 mM Tris-HCl pH 7.5, 25 mM MgCl2, 25 mM KC1, 10 mM 2-mercaptoethanol, at 30°C | Clarkia breweri | |
0.34 | - |
benzyl alcohol | isoform BEAT-2, in 250 mM Tris-HCl pH 7.5, 25 mM MgCl2, 25 mM KC1, 10 mM 2-mercaptoethanol, at 30°C | Clarkia concinna |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + benzyl alcohol | Clarkia breweri | 100% activity | CoA + benzyl acetate | - |
? | |
acetyl-CoA + benzyl alcohol | Clarkia concinna | 100% activity | CoA + benzyl acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clarkia breweri | - |
- |
- |
Clarkia concinna | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography | Clarkia breweri |
DEAE column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography | Clarkia concinna |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Clarkia breweri | - |
flower | - |
Clarkia concinna | - |
petal | - |
Clarkia breweri | - |
petal | - |
Clarkia concinna | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 2-(naphthalen-1-yl)ethanol | 139% activity compared to benzyl alcohol with isoform BEAT-1, 93% activity compared to benzyl alcohol with isoform BEAT-2, 217% activity compared to benzyl alcohol with isoform BEAT-3 | Clarkia concinna | CoA + 2-(naphthalen-1-yl)ethyl acetate | - |
? | |
acetyl-CoA + 2-phenylethanol | 11% activity compared to benzyl alcohol with isoform BEAT-1, 21% activity compared to benzyl alcohol with isoform BEAT-2, 131% activity compared to benzyl alcohol with isoform BEAT-3 | Clarkia concinna | CoA + 2-phenylethyl acetate | - |
? | |
acetyl-CoA + 2-phenylethanol | 28% activity compared to benzyl alcohol with isoform BEAT-1, 40% activity compared to benzyl alcohol with isoform BEAT-2 | Clarkia breweri | CoA + 2-phenylethyl acetate | - |
? | |
acetyl-CoA + 3-cis-hexene-1-ol | 102% activity compared to benzyl alcohol with isoform BEAT-1, 102% activity compared to benzyl alcohol with isoform BEAT-2 | Clarkia breweri | CoA + (3Z)-hex-3-en-1-yl acetate | - |
? | |
acetyl-CoA + 3-cis-hexene-1-ol | 70% activity compared to benzyl alcohol with isoform BEAT-1, 128% activity compared to benzyl alcohol with isoform BEAT-2, 66% activity compared to benzyl alcohol with isoform BEAT-3 | Clarkia concinna | CoA + (3Z)-hex-3-en-1-yl acetate | - |
? | |
acetyl-CoA + benzyl alcohol | 100% activity | Clarkia breweri | CoA + benzyl acetate | - |
? | |
acetyl-CoA + benzyl alcohol | 100% activity | Clarkia concinna | CoA + benzyl acetate | - |
? | |
acetyl-CoA + cinnamyl alcohol | 147% activity compared to benzyl alcohol with isoform BEAT-1, 162% activity compared to benzyl alcohol with isoform BEAT-2, 838% activity compared to benzyl alcohol with isoform BEAT-3 | Clarkia concinna | CoA + cinnamyl acetate | - |
? | |
acetyl-CoA + cinnamyl alcohol | 97% activity compared to benzyl alcohol with isoform BEAT-1, 122% activity compared to benzyl alcohol with isoform BEAT-2 | Clarkia breweri | CoA + cinnamyl acetate | - |
? | |
acetyl-CoA + heptanol | 143% activity compared to benzyl alcohol with isoform BEAT-1, 150% activity compared to benzyl alcohol with isoform BEAT-2 | Clarkia breweri | CoA + heptyl acetate | - |
? | |
acetyl-CoA + heptanol | 498% activity compared to benzyl alcohol with isoform BEAT-1, 528% activity compared to benzyl alcohol with isoform BEAT-2, 502% activity compared to benzyl alcohol with isoform BEAT-3 | Clarkia concinna | CoA + heptyl acetate | - |
? | |
acetyl-CoA + naphthalene ethanol | 57% activity compared to benzyl alcohol with isoform BEAT-1, 50% activity compared to benzyl alcohol with isoform BEAT-2 | Clarkia breweri | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetyl-CoA:benzylalcohol acetyltransferase | - |
Clarkia breweri |
acetyl-CoA:benzylalcohol acetyltransferase | - |
Clarkia concinna |
BEAT-1 | isoform | Clarkia breweri |
BEAT-1 | isoform | Clarkia concinna |
BEAT-2 | isoform | Clarkia breweri |
BEAT-2 | isoform | Clarkia concinna |
BEAT-3 | isoform | Clarkia concinna |
benzylalcohol acetyltransferase | - |
Clarkia breweri |
benzylalcohol acetyltransferase | - |
Clarkia concinna |