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Information on EC 2.1.1.371 - [histone H3]-lysine27 N-dimethyltransferase and Organism(s) Homo sapiens and UniProt Accession Q9BZ95

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IUBMB Comments
This entry describes enzymes that successively methylate the L-lysine27 residue of histone H3 (H3K27) twice, ultimately generating a dimethylated form. These modifications influence the binding of chromatin-associated proteins. The human NSD3 protein also catalyses the activity of EC 2.1.1.370, [histone H3]-lysine4 N-dimethyltransferase. cf. EC 2.1.1.369, [histone H3]-lysine27 N-methyltransferase, and EC 2.1.1.356, [histone H3]-lysine27 N-trimethyltransferase.
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Homo sapiens
UNIPROT: Q9BZ95
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The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
nsd3l, wolf-hirschhorn syndrome candidate 1-like 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Wolf-Hirschhorn syndrome candidate 1-like 1
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NSD3
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:[histone H3]-L-lysine27 N6-dimethyltransferase
This entry describes enzymes that successively methylate the L-lysine27 residue of histone H3 (H3K27) twice, ultimately generating a dimethylated form. These modifications influence the binding of chromatin-associated proteins. The human NSD3 protein also catalyses the activity of EC 2.1.1.370, [histone H3]-lysine4 N-dimethyltransferase. cf. EC 2.1.1.369, [histone H3]-lysine27 N-methyltransferase, and EC 2.1.1.356, [histone H3]-lysine27 N-trimethyltransferase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + [histone H3]-L-lysine27
S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine27
show the reaction diagram
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-
?
S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine27
S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine27
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + [histone H3]-L-lysine27
S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine27
show the reaction diagram
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-
-
?
S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine27
S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine27
show the reaction diagram
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?
additional information
?
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Nsd3 is specific for the histones H3K4 and H3K27 residues
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NSD3_HUMAN
1437
0
161613
Swiss-Prot
other Location (Reliability: 2)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
backbone HN, N, Calpha, C', and side chain Cbeta assignments of the methyltransferase domain encompassing residues Q1049-K1299 in complex with S-adenosyl-l-methionine
molecular modelling of the open-and-closed conformation of the catalytic domain
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
development of a modified Escherichia coli expression system
expression of the C-terminal domain in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
NSD3 expression is reduced by isoproterenol stimuli both in vitro and in vivo
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
synthesis
expression of NSDs via tagging with a human influenza hemagglutinin tag greatly improves the quality of the recombinant NSDs, resulting in more than 95% pure, stable, and active NSD-hemagglutinins, with an increase in production yield up to 22.4fold and up to 6.25 mg/l from LB Escherichia coli culture, and without further purification
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kim, S.M.; Kee, H.J.; Eom, G.H.; Choe, N.W.; Kim, J.Y.; Kim, Y.S.; Kim, S.K.; Kook, H.; Kook, H.; Seo, S.B.
Characterization of a novel WHSC1-associated SET domain protein with H3K4 and H3K27 methyltransferase activity
Biochem. Biophys. Res. Commun.
345
318-323
2006
Homo sapiens (Q9BZ95)
Manually annotated by BRENDA team
Zhou, Z.; Thomsen, R.; Kahns, S.; Nielsen, A.
The NSD3L histone methyltransferase regulates cell cycle and cell invasion in breast cancer cells
Biochem. Biophys. Res. Commun.
398
565-570
2010
Homo sapiens (Q9BZ95)
-
Manually annotated by BRENDA team
Li, Y.; Ng, H.; Ngo, A.; Liu, S.; Tan, Y.; Kwek, P.; Hung, A.; Joy, J.; Hill, J.; Keller, T.; Kang, C.
Backbone resonance assignments for the SET domain of human methyltransferase NSD3 in complex with its cofactor
Biomol. NMR Assign.
11
225-229
2017
Homo sapiens (Q9BZ95)
Manually annotated by BRENDA team
Morishita, M.; Mevius, D.; Di Luccio, E.
In vitro histone lysine methylation by NSD1, NSD2/MMSET/WHSC1 and NSD3/WHSC1L
BMC Struct. Biol.
14
25
2014
Homo sapiens (Q9BZ95)
Manually annotated by BRENDA team
Chen, K.; Jian, D.; Zhao, L.; Zang, X.; Song, W.; Ma, J.; Jia, Z.; Wang, X.; Gao, C.
Protective effect of histone methyltransferase NSD3 on ISO-induced cardiac hypertrophy
FEBS Lett.
593
2556-2565
2019
Homo sapiens (Q9BZ95)
Manually annotated by BRENDA team
Gonzalez-Pecchi, V.; Kwan, A.; Doyle, S.; Ivanov, A.; Du, Y.; Fu, H.
NSD3S stabilizes MYC through hindering its interaction with FBXW7
J. Mol. Cell Biol.
12
438-447
2020
Homo sapiens (Q9BZ95)
Manually annotated by BRENDA team
Liu, Z.; Piao, L.; Zhuang, M.; Qiu, X.; Xu, X.; Zhang, D.; Liu, M.; Ren, D.
Silencing of histone methyltransferase NSD3 reduces cell viability in osteosarcoma with induction of apoptosis
Oncol. Rep.
38
2796-2802
2017
Homo sapiens (Q9BZ95)
Manually annotated by BRENDA team
Shen, Y.; Morishita, M.; di Luccio, E.
High yield recombinant expression and purification of oncogenic NSD1, NSD2, and NSD3 with human influenza hemagglutinin tag
Protein Expr. Purif.
166
105506
2020
Homo sapiens (Q9BZ95)
Manually annotated by BRENDA team