The enzyme, studied from the bacterium Escherichia coli O157:H7, is a radical SAM (AdoMet) enzyme that is involved in heme degradation and iron utilization under anaerobic conditions. The enzyme uses two SAM molecules for the reaction. The first molecule is used to generate a 5′-deoxyadenosyl radical, which abstracts a hydrogen atom from the methyl group of the second SAM molecule. The newly formed methylene radical attacks the substrate, causing a rearrangement of the porphyrin ring that results in the liberation of iron.
The expected taxonomic range for this enzyme is: Escherichia coli
The enzyme, studied from the bacterium Escherichia coli O157:H7, is a radical SAM (AdoMet) enzyme that is involved in heme degradation and iron utilization under anaerobic conditions. The enzyme uses two SAM molecules for the reaction. The first molecule is used to generate a 5'-deoxyadenosyl radical, which abstracts a hydrogen atom from the methyl group of the second SAM molecule. The newly formed methylene radical attacks the substrate, causing a rearrangement of the porphyrin ring that results in the liberation of iron.
Substrates: ChuW uses a primary carbon radical to catalyze methyl transfer and rearrangement of the porphyrin ring, resulting in the liberation of iron and opening of the porphyrin ring Products: -
Substrates: the gene, and the corresponding protein, is part of a heme uptake and utilization operon that is common to several enteric pathogens Products: -
Substrates: the gene, and the corresponding protein, is part of a heme uptake and utilization operon that is common to several enteric pathogens Products: -
the oxygen-independent heme degradation pathway of Escherichia coli O157:H7 consists of ChuW, a radical SAM methyltransferase, which methylates heme to form the linear tetrapyrrole anaerobilin and liberate iron. ChuY uses anaerobilin as a substrate, reducing it to the tetrapyrrole termed anaerorubin
the gene, and the corresponding protein, is part of a heme uptake and utilization operon that is common to several enteric pathogens. The physiological role of the gene cluster (ChuW, ChuX, and ChuY) is the liberation of iron during colonization and infection of the intestine. ChuW catalyzes the anaerobic degradation of heme to release iron and produce a reactive tetrapyrrole termed anaerobilin
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