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Information on EC 2.1.1.2 - guanidinoacetate N-methyltransferase and Organism(s) Mus musculus and UniProt Accession O35969

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.2 guanidinoacetate N-methyltransferase
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Mus musculus
UNIPROT: O35969 not found.
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The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
guanidinoacetate methyltransferase, guanidinoacetate n-methyltransferase, guanidoacetate methyltransferase, n-guanidinoacetate methyltransferase, s-adenosyl-l-methionine:n-guanidinoacetate methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GA methylpherase
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-
-
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guanidinoacetate methyltransferase
guanidinoacetate N-methyltransferase
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guanidinoacetate transmethylase
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-
-
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guanidoacetate methyltransferase
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-
-
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methionine-guanidinoacetic transmethylase
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N-guanidinoacetate methyltransferase
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:N-guanidinoacetate methyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9029-75-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanidinoacetate
S-adenosyl-L-homocysteine + creatine
show the reaction diagram
S-adenosyl-L-methionine + guanidinoacetic acid + glutathione
?
show the reaction diagram
-
assay at pH 7.4, 37°C, 1 h, reaction stopped with 5% trichloroacetic acid
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-
?
additional information
?
-
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together with creatine/phosphocreatine shuttle system involved in brain energy homeostasis through a neuron-glial relationship
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanidinoacetate
S-adenosyl-L-homocysteine + creatine
show the reaction diagram
additional information
?
-
-
together with creatine/phosphocreatine shuttle system involved in brain energy homeostasis through a neuron-glial relationship
-
-
?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0005
-
liver of sarcoma-bearing mice
0.00053
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S180 cell
0.00057
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Ehrlich ascites carcinoma cell
0.0006
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
moderate expression levels throughout development
Manually annotated by BRENDA team
two waves of high GAMT expression. The first involves high expression in mitotic cells in the ventricular zone of the brain wall and the external granular layer of the cerebellum at the embryonic and neonatal stages. The second is initiated by striking up-regulation of GAMT in oligodendrocytes during the second and third postnatal weeks and continues to adulthood
Manually annotated by BRENDA team
GAMT levels are low to moderate in neuroblasts residing in the ventricular zone, increase during the second postnatal week, and decrease to very low levels thereafter
Manually annotated by BRENDA team
striking up-regulation of GAMT in oligodendrocytes during the second and third postnatal weeks, expression continues to adulthood
Manually annotated by BRENDA team
GAMT is highly expressed in perivascular pericytes and smooth muscle cells after birth
Manually annotated by BRENDA team
GAMT was highly expressed in perivascular pericytes and smooth muscle cells after birth
Manually annotated by BRENDA team
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highest enzyme level
Manually annotated by BRENDA team
-
low enzyme level
Manually annotated by BRENDA team
-
low enzyme level
Manually annotated by BRENDA team
-
S180 cells
Manually annotated by BRENDA team
-
mainly found in Sertoli cells of seminiferous tubules
Manually annotated by BRENDA team
-
low enzyme level
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GAMT_MOUSE
236
0
26336
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
-
SDS-PAGE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saos2, U2OS, and A-549 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
both RNA and protein levels of GAMT are increased in a p53-dependent manner after various inducers of genotoxic stress (etoposide, H2O2, and gamma-irradiation), glucose deprivation induces GAMT in a p53-dependent manner, GAMT mRNA and protein are increased in several tissues of glucose-deprived p53 wild-type mice while remaining unchanged in the same tissues of p53 null mice
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GAMT induction in response to DNA damage is dependent upon p53, GAMT also shows p53-dependent induction in response to exogenously expressed p53 and genotoxic stress, metabolic stress induces GAMT expression to regulate creatine levels in a p53-dependent manner
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high expression in tumor cells
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lee, H.; Ogawa, H.; Fujioka, M.; Gerton, G.L.
Guanidinoacetate methyltransferase in the mouse: extensive expression in Sertoli cells of testis and in microvilli of caput epididymis
Biol. Reprod.
50
152-162
1994
Mus musculus
Manually annotated by BRENDA team
Tachikawa, M.; Fukaya, M.; Terasaki, T.; Ohtsuki, S.; Watanabe, M.
Distinct cellular expressions of creatine synthetic enzyme GAMT and creatine kinases uCK-Mi and CK-B suggest a novel neuron-glial relationship for brain energy homeostasis
Eur. J. Neurosci.
20
144-160
2004
Mus musculus
Manually annotated by BRENDA team
Kan, H.E.; Meeuwissen, E.; van Asten, J.J.; Veltien, A.; Isbrandt, D.; Heerschap, A.
Creatine uptake in brain and skeletal muscle of mice lacking guanidinoacetate methyltransferase assessed by magnetic resonance spectroscopy
J. Appl. Physiol.
102
2121-2127
2007
Mus musculus
Manually annotated by BRENDA team
Schneider, J.E.; Stork, L.A.; Bell, J.T.; Hove, M.T.; Isbrandt, D.; Clarke, K.; Watkins, H.; Lygate, C.A.; Neubauer, S.
Cardiac structure and function during ageing in energetically compromised Guanidinoacetate N-methyltransferase (GAMT)-knockout mice - a one year longitudinal MRI study
J. Cardiovasc. Magn. Reson.
10
1-9
2008
Mus musculus
Manually annotated by BRENDA team
Braissant, O.; Henry, H.
AGAT, GAMT and SLC6A8 distribution in the central nervous system, in relation to creatine deficiency syndromes: A review
J. Inherit. Metab. Dis.
31
230-239
2008
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Heerschap, A.; Kan, H.E.; Nabuurs, C.I.; Renema, W.K.; Isbrandt, D.; Wieringa, B.
In vivo magnetic resonance spectroscopy of transgenic mice with altered expression of guanidinoacetate methyltransferase and creatine kinase isoenzymes
Subcell. Biochem.
46
119-148
2007
Mus musculus
Manually annotated by BRENDA team
Bera, S.; Wallimann, T.; Ray, S.; Ray, M.
Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells
FEBS J.
275
5899-5909
2008
Mus musculus
Manually annotated by BRENDA team
Zhu, Y.; Prives, C.
p53 and Metabolism: The GAMT connection
Mol. Cell
36
351-352
2009
Mus musculus
Manually annotated by BRENDA team
Ide, T.; Brown-Endres, L.; Chu, K.; Ongusaha, P.P.; Ohtsuka, T.; El-Deiry, W.S.; Aaronson, S.A.; Lee, S.W.
GAMT, a p53-inducible modulator of apoptosis, is critical for the adaptive response to nutrient stress
Mol. Cell
36
379-392
2009
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Tachikawa, M.; Watanabe, M.; Fukaya, M.; Sakai, K.; Terasaki, T.; Hosoya, K.I.
Cell-type-specific spatiotemporal expression of creatine biosynthetic enzyme S-adenosylmethionine guanidinoacetate N-methyltransferase in developing mouse brain
Neurochem. Res.
43
500-510
2018
Mus musculus (O35969)
Manually annotated by BRENDA team