cloned from the VIN13 commercial wine yeast strain, and placed under the constitutive control of the PGK1 regulatory sequences to generate plasmids pATF2-s. Overexpression of ATF2 in vine yeast increased the concentrations of ethyl acetate and isoamyl acetate to a lesser degree than overexpression of ATF1
cloned from the VIN13 commercial wine yeast strain, and placed under the constitutive control of the PGK1 regulatory sequences. Overexpression of ATF1 in vine yeast significantly increased the concentrations of ethyl acetate, isoamyl acetate, 2-phenylethyl acetate and ethyl caproate
gene atf1, recombinant coexpression of the enzyme with genes YjgB, KDC, ARO8, SerA, ARoG, and TrpABCD from Saccharomyces cerevisiae strain YPH499 in Escherichia coli strain MG1655 to establish the production of indole-3-ethanol and indole-3-ethanol acetate from glucose, method, overview. Subcloning in Escherichia coli strain DH1013
overexpressed in Saccharomyces cerevisiae: Gas chromatographic analysis of small-scale fermentations with the transformant strain shows that KlATF overexpression only causes a moderate increase in acetate esters compared to Saccharomyces cerevisiae ATF1 overexpression results in a substantial increase in acetate ester levels