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Literature summary for 2.3.1.84 extracted from

  • Dexter, R.; Qualley, A.; Kish, C.M.; Ma, C.J.; Koeduka, T.; Nagegowda, D.A.; Dudareva, N.; Pichersky, E.; Clark, D.
    Characterization of a petunia acetyltransferase involved in the biosynthesis of the floral volatile isoeugenol (2007), Plant J., 49, 265-75.
    View publication on PubMed
Search for more literature for 2.3.1.84

Cloned(Commentary)

Cloned (Comment) Organism
complete coding region of PhCFAT cDNA subcloned into expression vector pET-28a and expressed in Escherichia coli Petunia x hybrida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0226
-
 acetyl-CoA apparent KM value of recombinant protein with benzyl alcohol, pH 7.5, 20°C Petunia x hybrida
0.0275
-
 coniferyl alcohol apparent KM value of recombinant protein with acetyl-CoA, pH 7.5, 20°C Petunia x hybrida
0.0306
-
 acetyl-CoA apparent KM value of recombinant protein with coniferyl alcohol, pH 6.0, 20°C Petunia x hybrida
0.0441
-
 acetyl-CoA apparent KM value of recombinant protein with coniferyl alcohol, pH 7.5, 20°C Petunia x hybrida
0.0451
-
 acetyl-CoA apparent KM value of recombinant protein with 2-phenylethanol, pH 7.5, 20°C Petunia x hybrida
0.0565
-
 coniferyl alcohol apparent KM value of recombinant protein with acetyl-CoA, pH 6.0, 20°C Petunia x hybrida
3.6
-
 2-phenylethanol apparent KM value of recombinant protein with acetyl-CoA, pH 7.5, 20°C Petunia x hybrida
4.5
-
 benzyl alcohol apparent KM value of recombinant protein with acetyl-CoA, pH 7.5, 20°C Petunia x hybrida

Organism

Organism UniProt Comment Textmining
Petunia x hybrida A1XWY7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein purified to 99% homogeneity using nickel-based affinity chromatography Petunia x hybrida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-octanol + acetyl-CoA
-
 Petunia x hybrida acetyl-CoA + 1-octanol
-
 ?
2-phenylethanol + acetyl-CoA
-
 Petunia x hybrida 2-phenylethyl acetate + CoA
-
 ?
benzyl alcohol + acetyl-CoA
-
 Petunia x hybrida benzyl acetate + CoA
-
 ?
cinnamyl alcohol + acetyl-CoA
-
 Petunia x hybrida cinnamyl acetate + CoA
-
 ?
coniferyl alcohol + acetyl-CoA
-
 Petunia x hybrida coniferyl acetate + CoA
-
 ?
geraniol + acetyl-CoA
-
 Petunia x hybrida geranyl acetate + CoA
-
 ?
sinapyl alcohol + acetyl-CoA 71% of the activity with coniferyl alcohol Petunia x hybrida sinapyl acetate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
PhCFAT
-
 Petunia x hybrida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.81
-
 coniferyl alcohol recombinant protein with acetyl-CoA, pH 7.5, 20°C Petunia x hybrida
0.82
-
 benzyl alcohol recombinant protein with acetyl-CoA, pH 7.5, 20°C Petunia x hybrida
0.91
-
 2-phenylethanol recombinant protein with acetyl-CoA, pH 7.5, 20°C Petunia x hybrida
0.93
-
 acetyl-CoA recombinant protein with 2-phenylethanol, pH 7.5, 20°C Petunia x hybrida
1.13
-
 acetyl-CoA recombinant protein with benzyl alcohol, pH 7.5, 20°C Petunia x hybrida
1.51
-
 acetyl-CoA recombinant protein with coniferyl alcohol, pH 7.5, 20°C Petunia x hybrida
2.05
-
 coniferyl alcohol recombinant protein with acetyl-CoA, pH 6.0, 20°C Petunia x hybrida
2.32
-
 acetyl-CoA recombinant protein with coniferyl alcohol, pH 6.0, 20°C Petunia x hybrida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 highest activity with coniferyl alcohol Petunia x hybrida

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8.5
-
 Petunia x hybrida

Expression

Organism Comment Expression
Petunia x hybrida PhCFAT transcript levels decrease after ethylene release, diurnal rhythm of PhCFAT expression down