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2.1.3.2: aspartate carbamoyltransferase

This is an abbreviated version!
For detailed information about aspartate carbamoyltransferase, go to the full flat file.

Word Map on EC 2.1.3.2

Reaction

Carbamoyl phosphate
+
L-aspartate
=
phosphate
 +
N-carbamoyl-L-aspartate

Synonyms

(S)-2-methyl-3-oxopropanoyl-CoA:pyruvate carboxyltransferase, ACT, aspartate carbamoyltransferase, aspartate carbamyltransferase, aspartate trans carbamoylase, aspartate transcarbamoylase, aspartate transcarbamylase, aspartic acid transcarbamoylase, aspartic carbamyltransferase, aspartic transcarbamylase, ATC, ATC domain of CAD, ATCase, CAD, carbamoylaspartotranskinase, carbamoyltransferase, aspartate, carbamylaspartotranskinase, L-aspartate transcarbamoylase, L-aspartate transcarbamylase, MJ1581, PYRB

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.3 Carboxy- and carbamoyltransferases
                2.1.3.2 aspartate carbamoyltransferase

Cloned

Cloned on EC 2.1.3.2 - aspartate carbamoyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ATCase overexpression in Escherichia coli strain strain EK1104
coexpression of carbamoylphosphate synthetase II, aspartate transcarbamoylase, and dihydroorotase in Escherichia coli
-
crystallized by the microdialysis method. The structures are determined by X-ray crystallography at 2.15 resolution for His20Ar (histidine 20 of the regulatory (r) chain mutated to alanine) and and 2.75 A resolution for Asp19Ar (asparagine 19 of the regulatory (r) chain mutated to alanine)
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells
expression in Escherichia coli
expression in Escherichia coli strain BL21
expression in Escherichia coli. The catalytic and regulatory functions which, in the class B ATCases from members of the Enterobacteriaceae, Vibrio sp., and archaea, are carried out by specialized polypeptides, are combined on a single type of polypeptide assembled in trimers
expression in Eshcerichia coli
-
expression of catalytic subunit in Escherichia coli
expression of the catalytic trimer in Escherichia coli strain EK1911, which has a deletion in the pyrBI region of the chromosome and contains plasmids pEK406 coding for the Methanococcus jannaschii ATCase catalytic chain and pSJS1240
expression of the regulatiry subunit in Escherichia coli ATCase-deficient derivative strain C600 which contains T7 RNA polymerase gene under lacUV5 control as a lambda lysogen
expression of wild-type and mutant enzymes in Escherichia coli strain EK1104 containing the plasmid pEK695
gene pyrB, recombinant enzyme overexpression in Escherichia coli strain EK1104
overexpression in Escherichia coli
overexpression in Escherichia coli strain EK1104 transformed with plasmid pEK15212 containing the Escherichia coli pyrBI gene
recombinant expression of the enzyme as part of the N-terminal His6-tagged maltose-binding fusion protein CAD complex in Escherichia coli strain BL21(DE3) pLysS cells