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2.1.3.2: aspartate carbamoyltransferase

This is an abbreviated version!
For detailed information about aspartate carbamoyltransferase, go to the full flat file.

Word Map on EC 2.1.3.2

Reaction

Carbamoyl phosphate
+
L-aspartate
=
phosphate
 +
N-carbamoyl-L-aspartate

Synonyms

(S)-2-methyl-3-oxopropanoyl-CoA:pyruvate carboxyltransferase, ACT, aspartate carbamoyltransferase, aspartate carbamyltransferase, aspartate trans carbamoylase, aspartate transcarbamoylase, aspartate transcarbamylase, aspartic acid transcarbamoylase, aspartic carbamyltransferase, aspartic transcarbamylase, ATC, ATC domain of CAD, ATCase, CAD, carbamoylaspartotranskinase, carbamoyltransferase, aspartate, carbamylaspartotranskinase, L-aspartate transcarbamoylase, L-aspartate transcarbamylase, MJ1581, PYRB

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.3 Carboxy- and carbamoyltransferases
                2.1.3.2 aspartate carbamoyltransferase

General Stability

General Stability on EC 2.1.3.2 - aspartate carbamoyltransferase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
50% glycerol stabilizes
-
carbamoyl phosphate protects against proteolysis
-
carbamoyl phosphate protects against thermal denaturation
-
carboxylic acids, e.g. acetic acid, citric acid and high ionic strength, 200 mM KCl, protect against thermal denaturation at 65-70°C
-
chemical stabilization of conformational states of enzyme
-
cold-labile when highly purified, must be stored at room temperature, complete loss of activity after 24 h at pH 7.0 and 0°C
-
dimethylsulfoxid and glycerol are required to stabilize the enzyme
-
freezing and thawing in 50 mM Tris-HCl buffer, pH 7.4, 1 mM 2-mercaptoethanol has no effect on stability
-
phosphate required for maximum stability
-
presence of 20 mM potassium phosphate as well as 10% v/v glycerol is required for maintenance of activity of the purified enzyme
-
purified enzyme is inactivated if stored frozen
-
thermal denaturation
-
unstable in very dilute solutions
-