General Stability | Organism |
---|---|
phosphate required for maximum stability | Vigna radiata var. radiata |
presence of 20 mM potassium phosphate as well as 10% v/v glycerol is required for maintenance of activity of the purified enzyme | Vigna radiata var. radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
carbamoyl aspartate | - |
Vigna radiata var. radiata | |
Guanidine-HCl | 800 mM, almost complete inhibition | Vigna radiata var. radiata | |
p-chloromercuribenzoate | 6 mM, almost complete inhibition | Vigna radiata var. radiata | |
phosphate | - |
Vigna radiata var. radiata | |
succinate | activator at low concentrations of both succinate and aspartate, inhibitor at high succinate concentrations and at high aspartate concentrations | Vigna radiata var. radiata | |
UMP | - |
Vigna radiata var. radiata | |
Urea | 2.5 M, almost complete inhibition | Vigna radiata var. radiata | |
UTP | - |
Vigna radiata var. radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.091 | - |
Carbamoyl phosphate | - |
Vigna radiata var. radiata | |
1.7 | - |
L-aspartate | - |
Vigna radiata var. radiata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
gel filtration | Vigna radiata var. radiata |
93000 | - |
glycerol gradient | Vigna radiata var. radiata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoylphosphate + L-aspartate | Vigna radiata var. radiata | - |
phosphate + N-carbamoyl-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata var. radiata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, DEAE-Sephadex, Sephadex G-200, partially purified | Vigna radiata var. radiata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate | reaction mechanism | Vigna radiata var. radiata | |
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate | kinetic data suggest an ordered bi bi mechanism | Vigna radiata var. radiata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.35 | - |
- |
Vigna radiata var. radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-aspartate | - |
Vigna radiata var. radiata | phosphate + N-carbamoyl-L-aspartate | - |
? | |
carbamoylphosphate + L-aspartate | - |
Vigna radiata var. radiata | phosphate + N-carbamoyl-L-aspartate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Vigna radiata var. radiata |