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Literature summary for 2.1.3.2 extracted from

  • Ong, B.L.; Jackson, J.F.
    Aspartate transcarbmoylase from Phaseolus aureus (1972), Biochem. J., 129, 571-581.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
phosphate required for maximum stability Vigna radiata var. radiata
presence of 20 mM potassium phosphate as well as 10% v/v glycerol is required for maintenance of activity of the purified enzyme Vigna radiata var. radiata

Inhibitors

Inhibitors Comment Organism Structure
carbamoyl aspartate
-
Vigna radiata var. radiata
Guanidine-HCl 800 mM, almost complete inhibition Vigna radiata var. radiata
p-chloromercuribenzoate 6 mM, almost complete inhibition Vigna radiata var. radiata
phosphate
-
Vigna radiata var. radiata
succinate activator at low concentrations of both succinate and aspartate, inhibitor at high succinate concentrations and at high aspartate concentrations Vigna radiata var. radiata
UMP
-
Vigna radiata var. radiata
Urea 2.5 M, almost complete inhibition Vigna radiata var. radiata
UTP
-
Vigna radiata var. radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.091
-
Carbamoyl phosphate
-
Vigna radiata var. radiata
1.7
-
L-aspartate
-
Vigna radiata var. radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
gel filtration Vigna radiata var. radiata
93000
-
glycerol gradient Vigna radiata var. radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoylphosphate + L-aspartate Vigna radiata var. radiata
-
phosphate + N-carbamoyl-L-aspartate
-
?

Organism

Organism UniProt Comment Textmining
Vigna radiata var. radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, DEAE-Sephadex, Sephadex G-200, partially purified Vigna radiata var. radiata

Reaction

Reaction Comment Organism Reaction ID
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Vigna radiata var. radiata
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate kinetic data suggest an ordered bi bi mechanism Vigna radiata var. radiata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.35
-
-
Vigna radiata var. radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-aspartate
-
Vigna radiata var. radiata phosphate + N-carbamoyl-L-aspartate
-
?
carbamoylphosphate + L-aspartate
-
Vigna radiata var. radiata phosphate + N-carbamoyl-L-aspartate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
Vigna radiata var. radiata