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Literature summary for 2.1.3.2 extracted from

  • Xu, Y.; Zhang, Y.; Liang, Z.; Van de Casteele, M.; Legrain, C.; Glansdorff, N.
    Aspartate carbamoyltransferase from a psychrophilic deep-sea bacterium, Vibrio strain 2693: properties of the enzyme, genetic organization and synthesis in Escherichia coli (1998), Microbiology, 144, 1435-1441.
No PubMed abstract available
Search for more literature for 2.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vibrio sp.

Inhibitors

Inhibitors Comment Organism Structure
CTP
-
 Vibrio sp.
Phenylglyoxal
-
 Vibrio sp.
succinate
-
 Vibrio sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 Carbamoyl phosphate
-
 Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
-
 Vibrio natriegens
320000
-
 gel filtration Vibrio sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoylphosphate + L-aspartate Vibrio sp.
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Vibrio natriegens
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Vibrio sp. 2693
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio natriegens
-
-
-
Vibrio sp.
-
 isolated from the deep atlantic
-
Vibrio sp. 2693
-
 isolated from the deep atlantic
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant enzyme Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-aspartate
-
 Vibrio sp. phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Vibrio natriegens phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Vibrio sp. 2693 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Vibrio sp. phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Vibrio natriegens phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Vibrio sp. 2693 phosphate + N-carbamoyl-L-aspartate
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2 50 20% of maximal activity at 2°C, 30% at 6°C Vibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 more than 90% inactivation after 15 min, half-life: 4 min Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10
-
 Vibrio sp.