Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
truncated enzyme, hanging drop vapor diffusion method, using 0.2 M sodium sulfate, 5 mM MgSO4, 15% (w/v) PEG 3350 in 0.1 M bis-Tris propane pH 7.5 | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
R109A/K138A | the mutant shows significantly lower activity compared to the wild type enzyme | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-aspartate | Plasmodium falciparum | - |
phosphate + N-carbamoyl-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | O15804 | - |
- |
Purification (Comment) | Organism |
---|---|
Strep-tag affinity resin column chromatography and Superdex 75 gel filtration | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.85 | - |
purified mutant enzyme R109A/K138A, at pH 8.0 and 25°C | Plasmodium falciparum |
11.04 | - |
purified truncated wild type enzyme, at pH 8.0 and 25°C | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-aspartate | - |
Plasmodium falciparum | phosphate + N-carbamoyl-L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 43300, calculated from amino acid sequence | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
aspartate transcarbamoylase | - |
Plasmodium falciparum |
ATC | - |
Plasmodium falciparum |