EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.4.19.1 | -999 |
- |
coupling to CNBr-Sepharose and cross-linking with dimethylsuberimidate improve the thermal stability |
95260 |
| 3.4.19.1 | -999 |
- |
thermal stability of the multi-domain SpAAP is investigated by intrinsic fluorescence spectroscopy. This technique suggests a cooperative and apparently two-state unfolding process with a Tm of 50°C, which is consistent with the results previously observed by CD spectroscopy |
731358 |
| 3.4.19.1 | 0 |
100 |
the major conformation of the enzyme remains stable |
95261 |
| 3.4.19.1 | 10 |
20 |
enzyme retains 80% activity after 7h of incubation |
731801 |
| 3.4.19.1 | 37 |
- |
complete inactivation after 5 h of incubation |
731801 |
| 3.4.19.1 | 37 |
- |
residual activity halved after 5 h of incubation |
731801 |
| 3.4.19.1 | 40 |
- |
half-life of the purified recombinant enzyme is 29 h |
753251 |
| 3.4.19.1 | 50 |
- |
72 h, stable |
720431 |
| 3.4.19.1 | 50 |
- |
half-life of a few min |
731801 |
| 3.4.19.1 | 70 |
- |
24 h, 70% loss of activity. Slight increase its relative activity during the first 5 hours of incubation |
720867 |
