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Literature summary for 3.4.19.1 extracted from

  • Ishikawa, K.; Ishida, H.; Koyama, Y.; Kawarabayasi, Y.; Kawahara, J.; Matsui, E.; Matsui, I.
    Acylamino acid-releasing enzyme from the thermophilic archaeon Pyrococcus horikoshii (1998), J. Biol. Chem., 273, 17726-17731.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6
-
N-acetyl-Ala-Ala
-
Pyrococcus horikoshii
11
-
N-acetyl-Leu p-nitroanilide
-
Pyrococcus horikoshii
13
-
N-acetyl-Ala-Ala-Ala-Ala
-
Pyrococcus horikoshii
18.4
-
N-acetyl-Ala p-nitroanilide
-
Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
260000
-
heat-activated enzyme, light-scattering photometry Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formylalanine-Ala-Ala + H2O
-
Pyrococcus horikoshii ?
-
?
formylalanine-Ala-Ala-Ala-Ala-Ala + H2O
-
Pyrococcus horikoshii ?
-
?
formylmethionine-Ala + H2O
-
Pyrococcus horikoshii formylmethionine + Ala
-
?
formylmethionine-Ala-Ala-Ala-Ala-Ala + H2O
-
Pyrococcus horikoshii ?
-
?
formylmethionine-Ala-Ser + H2O
-
Pyrococcus horikoshii ?
-
?
formylmethionine-Leu-Gly + H2O
-
Pyrococcus horikoshii ?
-
?
N-acetyl-Ala p-nitroanilide + H2O
-
Pyrococcus horikoshii N-acetyl-Ala + p-nitroaniline
-
?
N-acetyl-Ala-Ala + H2O
-
Pyrococcus horikoshii N-acetyl-Ala + Ala
-
?
N-acetyl-Ala-Ala-Ala-Ala + H2O
-
Pyrococcus horikoshii N-acetyl-Ala + Ala-Ala-Ala
-
?
N-acetyl-Ala-Ala-Ala-Ala-Ala-Ala + H2O
-
Pyrococcus horikoshii ?
-
?
N-acetyl-Leu p-nitroanilide + H2O
-
Pyrococcus horikoshii N-acetyl-Leu + p-nitroaniline
-
?
N-acetyl-Met-Ala + H2O
-
Pyrococcus horikoshii N-acetyl-Met + Ala
-
?
N-acetyl-Met-Ala-Ala-Ala-Ala-Ala + H2O
-
Pyrococcus horikoshii ?
-
?
N-acetyl-Tyr p-nitroanilide + H2O
-
Pyrococcus horikoshii N-acetyl-Tyr + p-nitroaniline
-
?

Synonyms

Synonyms Comment Organism
AAREP
-
Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
at pH 5.4 Pyrococcus horikoshii
95
-
heat-activated enzyme Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
100 the major conformation of the enzyme remains stable Pyrococcus horikoshii
95
-
24 h, stable Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.3
-
N-acetyl-Ala p-nitroanilide
-
Pyrococcus horikoshii
281
-
N-acetyl-Ala-Ala-Ala-Ala
-
Pyrococcus horikoshii
330
-
N-acetyl-Leu p-nitroanilide
-
Pyrococcus horikoshii
790
-
N-acetyl-Ala-Ala
-
Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8 5.5 at 85°C Pyrococcus horikoshii
7
-
heat activated enzyme Pyrococcus horikoshii

pH Range

pH Minimum pH Maximum Comment Organism
5 8 about 60% of maximal activity at pH 5 and pH 8 Pyrococcus horikoshii