Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.19.1 extracted from

  • Gogliettino, M.; Balestrieri, M.; Cocca, E.; Mucerino, S.; Rossi, M.; Petrillo, M.; Mazzella, E.; Palmieri, G.
    Identification and characterisation of a novel acylpeptide hydrolase from Sulfolobus solfataricus: structural and functional insights (2012), PLoS One, 7, e37921.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Guanidine-HCl 1 M, complete loss of activity, after removal of guanidine-HCl the enzyme recovers 25% of its activity Saccharolobus solfataricus
phosphatidylethanolamine-binding protein inhibitor SsCEI
-
Saccharolobus solfataricus
Sulfolobus solfataricus chymotrypsin-elastase inhibitor specific inhibition Saccharolobus solfataricus
Urea 6 M, about 70% loss of activity, regains activity at almost all urea concentrations upon removal of the denaturinf agent Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
N-acetyl-L-Phe-4-nitroanilide in 25 mM Tris-HCl buffer pH 7.5, at 90°C Saccharolobus solfataricus
0.6
-
N-acetyl-L-leucyl 4-nitroanilide pH 7.5, 90°C Saccharolobus solfataricus
0.6
-
N-acetyl-L-Leu-4-nitroanilide in 25 mM Tris-HCl buffer pH 7.5, at 90°C Saccharolobus solfataricus
0.8
-
N-acetyl-L-alanyl 4-nitroanilide pH 7.5, 90°C Saccharolobus solfataricus
0.8
-
N-acetyl-L-Ala-4-nitroanilide in 25 mM Tris-HCl buffer pH 7.5, at 90°C Saccharolobus solfataricus
4
-
N-acetyl-L-phenylalanyl 4-nitroanilide pH 7.5, 90°C Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Saccharolobus solfataricus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
x * 64000, SDS-PAGE Saccharolobus solfataricus
64000
-
3 * 64000, SDS-PAGE Saccharolobus solfataricus
67000
-
3 * 67000, calculated from sequence Saccharolobus solfataricus
67000
-
x * 67000, calculated from amino acid sequence Saccharolobus solfataricus
192000
-
gel filtration Saccharolobus solfataricus

Organic Solvent Stability

Organic Solvent Comment Organism
guanidine-HCl 1 M, complete loss of activity, after removal of guanidine-HCl the enzyme recovers 25% of its activity Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97VD6
-
-
Saccharolobus solfataricus P2 Q97VD6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus
Q Sepharose column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, and Mono S column chromatography Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0007
-
cellular extract, using N-acetyl-L-Leu-4-nitroanilide as substrate, at pH 7.5 and 90°C Saccharolobus solfataricus
4.8
-
pH 7.5, 90°C Saccharolobus solfataricus
4.8
-
after 6857fold purification, using N-acetyl-L-Leu-4-nitroanilide as substrate, at pH 7.5 and 90°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with L-leucyl 4-nitroanilide, L-alanyl 4-nitroanilide or L-phenylalanyl 4-nitroanilide. The enzyme also shows endopeptidase activity Saccharolobus solfataricus ?
-
?
additional information no activity with L-Leu-4-nitroanilide, L-Ala-4-nitroanilide, or L-Phe-4-nitroanilide, succinyl-AAPF-4-nitroanilide, and succinyl-AAVA-4-nitroanilide Saccharolobus solfataricus ?
-
?
additional information no activity with L-leucyl 4-nitroanilide, L-alanyl 4-nitroanilide or L-phenylalanyl 4-nitroanilide. The enzyme also shows endopeptidase activity Saccharolobus solfataricus P2 ?
-
?
additional information no activity with L-Leu-4-nitroanilide, L-Ala-4-nitroanilide, or L-Phe-4-nitroanilide, succinyl-AAPF-4-nitroanilide, and succinyl-AAVA-4-nitroanilide Saccharolobus solfataricus P2 ?
-
?
N-acetyl-L-Ala-4-nitroanilide + H2O
-
Saccharolobus solfataricus N-acetyl-L-Ala + 4-nitroaniline
-
?
N-acetyl-L-alanyl 4-nitroanilide + H2O
-
Saccharolobus solfataricus N-acetyl-L-alanine + 4-nitroaniline
-
?
N-acetyl-L-alanyl 4-nitroanilide + H2O
-
Saccharolobus solfataricus P2 N-acetyl-L-alanine + 4-nitroaniline
-
?
N-acetyl-L-Leu-4-nitroanilide + H2O
-
Saccharolobus solfataricus N-acetyl-L-Leu + 4-nitroaniline
-
?
N-acetyl-L-leucyl 4-nitroanilide + H2O
-
Saccharolobus solfataricus N-acetyl-L-leucine + 4-nitroaniline
-
?
N-acetyl-L-leucyl 4-nitroanilide + H2O
-
Saccharolobus solfataricus P2 N-acetyl-L-leucine + 4-nitroaniline
-
?
N-acetyl-L-Phe-4-nitroanilide + H2O
-
Saccharolobus solfataricus N-acetyl-L-Phe + 4-nitroaniline
-
?
N-acetyl-L-phenylalanyl 4-nitroanilide + H2O
-
Saccharolobus solfataricus N-acetyl-L-phenylalanine + 4-nitroaniline
-
?
N-acetyl-L-phenylalanyl 4-nitroanilide + H2O
-
Saccharolobus solfataricus P2 N-acetyl-L-phenylalanine + 4-nitroaniline
-
?
succinyl-AAA-4-nitroanilide + H2O
-
Saccharolobus solfataricus succinyl-AAA + 4-nitroaniline
-
?
succinyl-GGF-4-nitroanilide + H2O
-
Saccharolobus solfataricus succinyl-GGF + 4-nitroaniline
-
?
Z-GGL-4-nitroanilide + H2O
-
Saccharolobus solfataricus Z-GGL + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
homotrimer x * 64000, SDS-PAGE Saccharolobus solfataricus
homotrimer 3 * 64000, SDS-PAGE Saccharolobus solfataricus
homotrimer 3 * 67000, calculated from sequence Saccharolobus solfataricus
homotrimer x * 67000, calculated from amino acid sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
Acylaminoacyl-peptidase
-
Saccharolobus solfataricus
acylpeptide hydrolase
-
Saccharolobus solfataricus
APEH-3
-
Saccharolobus solfataricus
APEH-3Ss
-
Saccharolobus solfataricus
SSO2693
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 90 more than 50% activity between 60 and 90°C Saccharolobus solfataricus
80 95 80°C: about 65% of maximal activity, 95°C: about 50% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
24 h, 70% loss of activity. Slight increase its relative activity during the first 5 hours of incubation Saccharolobus solfataricus
70 90 the enzyme retains 30% activity following the incubation for 24 h at 70°C while the residual activity of the enzyme is halved after 4 h incubation at 90°C Saccharolobus solfataricus
90
-
4 h, 50% loss of activity Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
N-acetyl-L-Phe-4-nitroanilide in 25 mM Tris-HCl buffer pH 7.5, at 90°C Saccharolobus solfataricus
8
-
N-acetyl-L-phenylalanyl 4-nitroanilide pH 7.5, 90°C Saccharolobus solfataricus
14
-
N-acetyl-L-alanyl 4-nitroanilide pH 7.5, 90°C Saccharolobus solfataricus
17.5
-
N-acetyl-L-Ala-4-nitroanilide in 25 mM Tris-HCl buffer pH 7.5, at 90°C Saccharolobus solfataricus
18
-
N-acetyl-L-leucyl 4-nitroanilide pH 7.5, 90°C Saccharolobus solfataricus
18
-
N-acetyl-L-Leu-4-nitroanilide in 25 mM Tris-HCl buffer pH 7.5, at 90°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 more than 50% activity between pH 5.0 and 9.0 Saccharolobus solfataricus
7.5 8 pH 7.5: about 55% of maximal activity, pH 8.0: about 75% of maximal activity Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.008
-
phosphatidylethanolamine-binding protein inhibitor SsCEI pH 7.5, 90°C, substrate: acetyl-Leu-4-nitroanilide Saccharolobus solfataricus

Expression

Organism Comment Expression
Saccharolobus solfataricus expression of apeh-3 is down-regulated as an immediate response to stress down

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
succinyl-AAA-4-nitroanilide in 25 mM Tris-HCl buffer pH 7.5, at 90°C Saccharolobus solfataricus
0.7
-
Z-GGL-4-nitroanilide in 25 mM Tris-HCl buffer pH 7.5, at 90°C Saccharolobus solfataricus
1.3
-
succinyl-GGF-4-nitroanilide in 25 mM Tris-HCl buffer pH 7.5, at 90°C Saccharolobus solfataricus
2
-
N-acetyl-L-phenylalanyl 4-nitroanilide pH 7.5, 90°C Saccharolobus solfataricus
2
-
N-acetyl-L-Phe-4-nitroanilide in 25 mM Tris-HCl buffer pH 7.5, at 90°C Saccharolobus solfataricus
17.5
-
N-acetyl-L-alanyl 4-nitroanilide pH 7.5, 90°C Saccharolobus solfataricus
17.5
-
N-acetyl-L-Ala-4-nitroanilide in 25 mM Tris-HCl buffer pH 7.5, at 90°C Saccharolobus solfataricus
32.7
-
N-acetyl-L-leucyl 4-nitroanilide pH 7.5, 90°C Saccharolobus solfataricus
32.7
-
N-acetyl-L-Leu-4-nitroanilide in 25 mM Tris-HCl buffer pH 7.5, at 90°C Saccharolobus solfataricus