Application | Comment | Organism |
---|---|---|
medicine | the enzyme may constitute a new therapeutic target for the treatment of a number of pathologies | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 100 mM, 52% inhibition | Saccharolobus solfataricus | |
phosphatidylethanolamine-binding protein inhibitor SsCEI | - |
Saccharolobus solfataricus | |
SDS | above 0.3 mM, complete inhibition | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 80°C | Saccharolobus solfataricus | |
11.4 | - |
N-acetyl-L-alanyl 4-nitroanilide | pH 7.5, 80°C | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharolobus solfataricus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, SDS-PAGE | Saccharolobus solfataricus |
125000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q7LX61 | - |
- |
Saccharolobus solfataricus P2 | Q7LX61 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
pH 7.5, 80°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with L-leucyl 4-nitroanilide or L-alanyl 4-nitroanilide. The enzyme is able to hydrolyse N-succinyl-Gly-Gly-Phe 4-nitroanilide, showing endopeptidase activity | Saccharolobus solfataricus | ? | - |
? | |
additional information | no activity with L-leucyl 4-nitroanilide or L-alanyl 4-nitroanilide. The enzyme is able to hydrolyse N-succinyl-Gly-Gly-Phe 4-nitroanilide, showing endopeptidase activity | Saccharolobus solfataricus P2 | ? | - |
? | |
N-acetyl-L-alanyl 4-nitroanilide + H2O | - |
Saccharolobus solfataricus | N-acetyl-L-alanine + 4-nitroaniline | - |
? | |
N-acetyl-L-alanyl 4-nitroanilide + H2O | - |
Saccharolobus solfataricus P2 | N-acetyl-L-alanine + 4-nitroaniline | - |
? | |
N-acetyl-L-leucyl 4-nitroanilide + H2O | preference for N-acetyl-L-leucyl 4-nitroanilide over N-acetyl-L-alanyl 4-nitroanilide | Saccharolobus solfataricus | N-acetyl-L-leucine + 4-nitroaniline | - |
? | |
N-acetyl-L-leucyl 4-nitroanilide + H2O | preference for N-acetyl-L-leucyl 4-nitroanilide over N-acetyl-L-alanyl 4-nitroanilide | Saccharolobus solfataricus P2 | N-acetyl-L-leucine + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
acylpeptide hydrolase | - |
Saccharolobus solfataricus |
APEH | - |
Saccharolobus solfataricus |
apeH-2 | - |
Saccharolobus solfataricus |
sso2141 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
72 h, stable | Saccharolobus solfataricus |
70 | - |
72 h, stable | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 80°C | Saccharolobus solfataricus | |
5 | - |
N-acetyl-L-alanyl 4-nitroanilide | pH 7.5, 80°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | at 80°C | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
phosphatidylethanolamine-binding protein inhibitor SsCEI | pH 7.5, 80°C, substrate: N-acetyl-L-leucyl 4-nitroanilide | Saccharolobus solfataricus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00000002 | - |
pH 7.5, 80°C, substrate: N-acetyl-L-leucyl 4-nitroanilide | Saccharolobus solfataricus | phosphatidylethanolamine-binding protein inhibitor SsCEI |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
N-acetyl-L-alanyl 4-nitroanilide | pH 7.5, 80°C | Saccharolobus solfataricus | |
16.8 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 80°C | Saccharolobus solfataricus |