KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
acetyl-Met-7-amido-4-methylcoumarin | 50°C, pH 8 | Trematomus bernacchii | |
0.12 | - |
acetyl-Ala-4-nitroanilide | 15°C, pH 8 | Trematomus bernacchii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
SDS-PAGE | Trematomus bernacchii |
80000 | - |
SDS-PAGE | Trematomus bernacchii |
300000 | - |
gel filtration | Trematomus bernacchii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trematomus bernacchii | - |
- |
- |
Trematomus bernacchii | V5QZ74 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Trematomus bernacchii | - |
erythrocyte | - |
Trematomus bernacchii | - |
gill | - |
Trematomus bernacchii | - |
heart | - |
Trematomus bernacchii | - |
kidney | - |
Trematomus bernacchii | - |
liver | - |
Trematomus bernacchii | - |
liver | strong expression | Trematomus bernacchii | - |
ovary | - |
Trematomus bernacchii | - |
ovary | strong expression | Trematomus bernacchii | - |
spleen | - |
Trematomus bernacchii | - |
testis | - |
Trematomus bernacchii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Ala-4-nitroanilide + H2O | - |
Trematomus bernacchii | acetyl-Ala + 4-nitroaniline | - |
? | |
acetyl-Leu-4-nitroanilide + H2O | - |
Trematomus bernacchii | acetyl-Leu + 4-nitroaniline | - |
? | |
acetyl-Met-7-amido-4-methylcoumarin + H2O | - |
Trematomus bernacchii | acetyl-Met + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 80000 | Trematomus bernacchii |
tetramer | 4 * 75000 | Trematomus bernacchii |
Synonyms | Comment | Organism |
---|---|---|
acylpeptide hydrolase | - |
Trematomus bernacchii |
APEH-1 | - |
Trematomus bernacchii |
apeH-2 | - |
Trematomus bernacchii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Trematomus bernacchii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 20 | enzyme retains 80% activity after 7h of incubation | Trematomus bernacchii |
37 | - |
complete inactivation after 5 h of incubation | Trematomus bernacchii |
37 | - |
residual activity halved after 5 h of incubation | Trematomus bernacchii |
50 | - |
half-life of a few min | Trematomus bernacchii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
acetyl-Ala-4-nitroanilide | 50°C, pH 8 | Trematomus bernacchii | |
0.03 | - |
acetyl-Met-7-amido-4-methylcoumarin | 50°C, pH 8 | Trematomus bernacchii | |
0.09 | - |
acetyl-Leu-4-nitroanilide | 15°C, pH 8 | Trematomus bernacchii | |
1.7 | - |
acetyl-Ala-4-nitroanilide | 15°C, pH 8 | Trematomus bernacchii | |
7.74 | - |
acetyl-Met-7-amido-4-methylcoumarin | 50°C, pH 8 | Trematomus bernacchii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Trematomus bernacchii |
Organism | Comment | Expression |
---|---|---|
Trematomus bernacchii | strong expression in liver | up |
Trematomus bernacchii | strong expression in ovary | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.72 | - |
acetyl-Met-7-amido-4-methylcoumarin | 50°C, pH 8 | Trematomus bernacchii | |
4.76 | - |
acetyl-Ala-4-nitroanilide | 37°C, pH 8 | Trematomus bernacchii |