Information on EC 3.4.24.15 - thimet oligopeptidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.4.24.15
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RECOMMENDED NAME
GeneOntology No.
thimet oligopeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5-15 residues
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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endopeptidase; peptides, endopeptidase
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CAS REGISTRY NUMBER
COMMENTARY hide
110639-28-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
N22
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Manually annotated by BRENDA team
N22
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Geobacillus sp.
Mus musculus C57BL/6
C57BL/6 mice
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Manually annotated by BRENDA team
Mus musculus C57BL/6J
female C57BL?6J
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain PAO1 PA-14, gene PA4498
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(7-methoxycoumarin-4-acetyl)-Pro-Leu-Gly-Pro-dLys-(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
(7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
Geobacillus sp.
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?
(7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
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fluorogenic substrate
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?
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile + Arg-Arg-Ala-Lys-dinitrophenyl
show the reaction diagram
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-
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?
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu + Arg-Arg-Ala-Lys-dinitrophenyl
show the reaction diagram
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?
(7-methoxycoumarin-4-yl)acetyl-Leu-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH + H2O
?
show the reaction diagram
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in wild-type, clear preference for the 7-methoxycoumarin-4-acetyl-[Ala7, Lys(dinitrophenol)9]-bradykinin substrate over 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol and 7-methoxycoumarin-Leu-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH
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-
?
(7-methoxycoumarin-4-yl)actayl-Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-OH + H2O
?
show the reaction diagram
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?
(Gly-Pro-Leu)n
Gly-Pro-Leu
show the reaction diagram
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n = 2, 3, 4 and 5, the peptides are cleaved only at Leu-Gly bonds, activity is most rapid with n = 3 and slowest with n = 5. The cleavage fitts a sequential first-order model
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?
(o-aminobenzoyl)-AFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-AFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-AF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-DFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-DFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-DF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-EFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-EFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-EF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-ENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-ENKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-ENKPRRPYIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(ortho-aminobenzoyl)-ENKPR + RPYIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (ortho-aminobenzoyl)-KPRRP + YIQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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84% cleavage at R-R bond, 16% cleavage at P-Y bond
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?
(o-aminobenzoyl)-ENKPRRPYQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-ENKPR + RPYQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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16% cleavage at P-Y bond, 40% cleavage at Y-Q bond, 44% cleavage at R-R
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?
(o-aminobenzoyl)-FFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-FFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GASPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GASP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GDSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GDSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GESPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GESP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GFAPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFAP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GFDPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFDP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GFEPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFEP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFFPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFFP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFHPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFHP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFIPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFIP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GFLPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFLP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GFNPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFNP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GFPPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFPP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GFQPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFQP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFRPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFRP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSAFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSA + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSDFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSD + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSEFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSE + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSFFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSF + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSHFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSH + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSLFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSL + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSNFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSN + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPARQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + ARQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPDRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + DRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPDRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GFSPERQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + ERQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPERQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GFSPFAQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFDQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GF + SPFDQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFEQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FEQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFEQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFFQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FFQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFHQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FHQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFHQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GFSPFIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FIQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFLQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FLQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFNQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FNQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFNQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFQQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FQQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFQQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFR-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GF + SPFR-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
72% of the activity with (o-aminobenzoyl)-GFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
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?
(o-aminobenzoyl)-GFSPFRA-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRA-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GFSPFRE-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRE-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRF-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRF-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRI-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRI-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRL-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRL-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GFSPFRN-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRN-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRP-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRP-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRR-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRR-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRS-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FRS-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPFRSSRIGEIKEEQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSPFRS + SRIGEIKEEQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
14% of the activity with (o-aminobenzoyl)-GFSPFRSSRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
?
(o-aminobenzoyl)-GFSPFRSSRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSPFRS + SRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
8% of the activity with (o-aminobenzoyl)-GFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
?
(o-aminobenzoyl)-GFSPFSQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + FSQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPFSQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GFSPHRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + HRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GFSPIRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + IRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPIRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GFSPLRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + LRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GF + SPLRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GFSPNRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + NRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GFSPQRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + QRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GFSPRRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + RRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GFSPSRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSP + SRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GFSQFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSQ + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GFSRFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSR + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
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?
(o-aminobenzoyl)-GFSSFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GFSS + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GGFLRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GGFLRRDQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RDQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
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?
(o-aminobenzoyl)-GGFLRREQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + REQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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-
-
?
(o-aminobenzoyl)-GGFLRRFQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RFQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GGFLRR + FQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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cleavage at the R-R or at the R-X bond
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?
(o-aminobenzoyl)-GGFLRRHQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RHQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GGFLRR + HQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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cleavage at the R-R or at the R-X bond
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?
(o-aminobenzoyl)-GGFLRRIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RIQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GGFLRR + IQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
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cleavage at the R-R or at the R-X bond
-
?
(o-aminobenzoyl)-GGFLRRLQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RLQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GGFLRR + LQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
cleavage at the R-R or at the R-X bond
-
?
(o-aminobenzoyl)-GGFLRRNQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RNQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GGFLRR + NQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
cleavage at the R-R or at the R-X bond
-
?
(o-aminobenzoyl)-GGFLRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GGFLRRRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GGFLRRV-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RV-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
cleavage at the R-R or at the R-X bond
-
?
(o-aminobenzoyl)-GGFLRRVQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RVQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GGFLRRYQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GGFLR + RYQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-GGFLRR + YQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
cleavage at the R-R or at the R-X bond
-
?
(o-aminobenzoyl)-GHSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GHSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GISPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GISP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GLSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GLSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GNSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GNSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GPSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GPSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GQSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GQSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GRSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GRSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-GSSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-GSSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-HFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-HFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-IFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-IFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-IF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-KPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-LFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-LFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-LF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-LYENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-LYENKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-LYENKPRRPYILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-LYENKPRRP + YILQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (ortho-aminobenzoyl)-LYENKPRRPY + ILQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
68% cleavage at P-Y bond, 32% cleavage at Y-I bond
-
?
(o-aminobenzoyl)-NFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-NFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-NF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-NKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-NKPR + RPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-Phe-Arg-Lys-(dinitrophenyl-proline) + H2O
(o-aminobenzoyl)-Phe-Arg + Lys-(dinitrophenyl-proline)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-QFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-QFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-QF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-RFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-RFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-RPPGFSPFR-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-RPPGF + SPFR-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
33% of the activity with (o-aminobenzoyl)-GFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
?
(o-aminobenzoyl)-RPPGFSPFR-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-RPPGFSP + FR-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
(o-aminobenzoyl)-RPPGFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-RPPGFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
(o-aminobenzoyl)-RPPGFSPFRSSRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-RPPGFSPFRS + SRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
23% of the activity with (o-aminobenzoyl)-GFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
?
(o-aminobenzoyl)-SFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
(o-aminobenzoyl)-SFSP + FRQ-(N-(2,4-dinitrophenyl)ethylenediamine) + (o-aminobenzoyl)-SF + SPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
show the reaction diagram
-
-
-
?
2,4-dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
?
show the reaction diagram
2-aminobenzoyl-GFSHFRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-GFSH + FRQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-GGFLRRDQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
2-aminobenzoyl-GGFL + RRDQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + 2-aminobenzoyl-GGFLR + RDQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
-
absence of Ca2+, 37% cleavage of R-R bond and 63% cleavage of L-R bond. Presence of 50 mM Ca2+, 74% cleavage of R-R bond and 26% of L-R bond
-
-
?
2-aminobenzoyl-GGFLRRVQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
2-aminobenzoyl-GGFL + RRVQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + 2-aminobenzoyl-GGFLR + RVQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
-
-
absence of Ca2+, 64% cleavage of R-R bond and 36% cleavage of L-R bond. Presence of 50 mM Ca2+, 79% cleavage of R-R bond and 21% of L-R bond
-
?
4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg
4-phenylazobenzyloxycarbonyl-Pro-Leu + Gly-Pro-Arg
show the reaction diagram
4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg
4-phenylazobenzyloxycarbonyl-Pro + Leu-Gly-Pro-D-Arg
show the reaction diagram
-
-
-
?
4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg + H2O
?
show the reaction diagram
Geobacillus sp.
-
-
-
-
?
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-(2,4-ditritrophenyl)Lys
?
show the reaction diagram
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
7-methoxycoumarin-3-carboxylyl-Pro-Leu + Gly-Pro-D-Lys-dinitrophenyl
show the reaction diagram
7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin + H2O
?
show the reaction diagram
-
-
-
-
?
7-methoxycoumarin-4-acetyl-PLGPdK-(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
-
-
-
-
?
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
-
-
-
?
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-2,4-dinitrophenyl + H2O
?
show the reaction diagram
-
fluorogenic substrate
-
-
?
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol + H2O
?
show the reaction diagram
-
in wild-type, clear preference for the 7-methoxycoumarin-4-acetyl-[Ala7, Lys(dinitrophenol)9]-bradykinin substrate over 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol and 7-methoxycoumarin-Leu-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH
-
-
?
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl + H2O
?
show the reaction diagram
-
-
?
7-methoxycoumarin-4-acetyl-[Ala7, Lys(dinitrophenol)9]-bradykinin + H2O
?
show the reaction diagram
-
in wild-type, clear preference for the 7-methoxycoumarin-4-acetyl-[Ala7, Lys(dinitrophenol)9]-bradykinin substrate over 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol and 7-methoxycoumarin-Leu-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH
-
-
?
7-methoxycoumaryl-4-acetyl-Pro-Leu-Gly-D-Lys-(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
-
-
-
?
7-methoxycoumaryl-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
-
-
-
?
7-methoxycoumatin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
-
-
-
?
Abz-GFDPFRQ-EDDnp + H2O
?
show the reaction diagram
Abz-GFSAFRQ-EDDnp + H2O
Abz-GFSA + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSAFRQEDDnp + H2O
Abz-GFSAFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSDFRQ-EDDnp + H2O
Abz-GFSD + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSEFRQ-EDDnp + H2O
Abz-GFSE + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSEFRQEDDnp + H2O
Abz-GFSEFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSFFRQ-EDDnp + H2O
Abz-GFSF + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSFFRQEDDnp + H2O
Abz-GFSFFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSHFRQ-EDDnp + H2O
Abz-GFSH + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSHFRQEDDnp + H2O
Abz-GFSHFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSIFR-EDDnp + H2O
Abz-GFSIFR + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSIFRQ-EDDnp + H2O
Abz-GFSI + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSIFRQ-EDDnp + H2O
Abz-GFSIFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSLFRQEDDnp + H2O
Abz-GFSLFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPARQ-EDDnp + H2O
Abz-Gly-Phe + Ser-Ala + Ala-Arg-Gln-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPDRQ-EDDnp + H2O
Abz-Gly-Phe + Ser-Ala + Asp-Arg-Gln-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPERQ-EDDnp + H2O
Abz-Gly-Phe + Ser-Ala + Glu-Arg-Gln-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPFR-EDDnp + H2O
Abz-GFSPFR + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPFRQ-EDDnp + H2O
Abz-GFSP + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPFRQ-EDDnp + H2O
Abz-GFSPFRQ + EDDnp
show the reaction diagram
Abz-GFSPFRQ-EDDnp + H2O
Abz-Gly-Phe + Ser-Ala + Phe-Arg-Gln-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPHRQ-EDDnp + H2O
Abz-Gly-Phe + Ser-Ala + His-Arg-Gln-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPIRQ-EDDnp + H2O
Abz-Gly-Phe + Ser-Ala + Ile-Arg-Gln-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPLRQ-EDDnp + H2O
Abz-Gly-Phe + Ser-Ala + Leu-Arg-Gln-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPQRQ-EDDnp + H2O
Abz-Gly-Phe + Ser-Ala + Gln-Arg-Gln-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPRRQ-EDDnp + H2O
Abz-Gly-Phe + Ser-Ala + Arg-Arg-Gln-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSPSRQ-EDDnp + H2O
Abz-Gly-Phe + Ser-Ala + Ser-Arg-Gln-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSQFRQ-EDDnp + H2O
Abz-GFSQ + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSQFRQEDDnp + H2O
Abz-GFSQFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSRFRQ-EDDnp + H2O
Abz-GFSR + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSRFRQEDDnp + H2O
Abz-GFSRFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSSFRQ-EDDnp + H2O
Abz-GFSS + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSSFRQEDDnp + H2O
Abz-GFSSFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSWFRQ-EDDnp + H2O
Abz-GFSW + FRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSWFRQEDDnp + H2O
Abz-GFSWFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GFSYFRQEDDnp + H2O
Abz-GFSYFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-NKPRRPQEDDnp + H2O
Abz-NKPRRPQ + EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RPPGFSPFRQEDDnp + H2O
Abz-RPPGFSPFRQ + EDDnp
show the reaction diagram
-
-
-
-
?
adrenorphin
peptide fragments
show the reaction diagram
Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys + H2O
?
show the reaction diagram
alpha-neoendorphin + H2O
?
show the reaction diagram
aminobenzyl-Ala-Gly-Leu-Ala-nitrobenzylamide
aminobenzyl-Ala-Gly + Leu-Ala-nitrobenzylamide
show the reaction diagram
-
weak
-
?
amyloid precursor protein + H2O
?
show the reaction diagram
-
-
-
-
?
amyloid precursor protein-derived substrate
peptide fragments
show the reaction diagram
-
spanning the beta-secretase site Ile-Ser-Glu-Val-Lys-Met-Asp-Ala-Glu-Phe-Arg-His-Asp-Ser, cleavage occurs predominantly at Lys-Met and Glu-Phe bonds
-
?
Angiotensin I + H2O
?
show the reaction diagram
angiotensin I + H2O
angiotensin-(1-7) + ?
show the reaction diagram
Angiotensin II + H2O
?
show the reaction diagram
-
7% of the activity with bradykinin
-
?
angiotensin-I + H2O
?
show the reaction diagram
-
-
-
-
?
angiotensin-II + H2O
?
show the reaction diagram
-
-
-
-
?
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met + H2O
peptide fragments
show the reaction diagram
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg
show the reaction diagram
ASNENMETM + H2O
?
show the reaction diagram
-
-
-
?
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe + H2O
Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
show the reaction diagram
benzoyl-Arg-Ala-Ala-Phe-p-aminobenzoate
benzoyl-Arg + Ala-Ala-Phe-p-aminobenzoate
show the reaction diagram
-
-
-
?
benzoyl-Asp-Ala-Ala-Phe-p-aminobenzoate
benzoyl-Asp + Ala-Ala-Phe-p-aminobenzoate
show the reaction diagram
-
-
-
?
benzoyl-Gly-Ala-Ala-Gly-p-aminobenzoate
benzoyl-Gly + Ala-Ala-Gly-p-aminobenzoate
show the reaction diagram
benzoyl-Gly-Ala-Ala-Leu-p-aminobenzoate
benzoyl-Gly + Ala-Ala-Leu-p-aminobenzoate
show the reaction diagram
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
benzoyl-Gly + Ala-Ala-Phe-p-aminobenzoate
show the reaction diagram
benzoyl-Gly-Gly-Ala-Phe-p-aminobenzoate
benzoyl-Gly + Gly-Ala-Phe-p-aminobenzoate
show the reaction diagram
benzoyl-Gly-Lys-Arg-Ala-Ala-Phe-p-aminobenzoate
benzoyl-Gly-Lys-Arg + Ala-Ala-Phe-p-aminobenzoate
show the reaction diagram
-
-
-
?
benzoyl-Gly-Phe-Ala-Ala-Phe-p-aminobenzoate
benzoyl-Gly-Phe + Ala-Ala-Phe-p-aminobenzoate
show the reaction diagram
benzoyl-Gly-Phe-Ala-Phe-p-aminobenzoate
benzoyl-Gly + Phe-Ala-Phe-p-aminobenzoate
show the reaction diagram
benzoyl-Gly-Phe-Phe-Ala-Ala-Phe-p-aminobenzoate
benzoyl-Gly-Phe-Phe + Ala-Ala-Phe-p-aminobenzoate
show the reaction diagram
-
-
-
?
beta-neoendorphin + H2O
?
show the reaction diagram
Bradykinin + H2O
?
show the reaction diagram
cholecystokinin-8
peptide fragments
show the reaction diagram
Collagen + H2O
?
show the reaction diagram
corticotropin-like intermediate lobe peptide
peptide fragments
show the reaction diagram
CPI-0004Na + H2O
?
show the reaction diagram
-
tetrapeptidic prodrug of doxorubicin
-
-
?
dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
dinitrophenyl-Pro-Leu + Gly-Pro-Trp-D-Lys
show the reaction diagram
dynorphin 1-8 + H2O
?
show the reaction diagram
dynorphin A1-8 + H2O
?
show the reaction diagram
-
-
-
-
?
ELFADKVPKTAENFR + 2 H2O
ELFADKVPKTA + Glu-Asn + Phe-Arg
show the reaction diagram
-
intracellular peptide identified as a substrate by incubation of HEK-293 cell extract with recombinant enzyme. Peptide is derived from peptidylprolyl isomerase A
-
-
-
ELFSYLIEKVKR + H2O
ELFSYLIEK + VKR
show the reaction diagram
-
purified TOP efficiently cleaves the nardilysin-dependent 12- and 13-residue precursors PRA(190-201) (ELFSYLIEKVKR) and PRA(190-202) (ELFSYLIEKVKRK) directly after the epitope's C-terminal Lys198
-
-
?
ELFSYLIEKVKRK + H2O
ELFSYLIEK + VKRK
show the reaction diagram
-
purified TOP efficiently cleaves the nardilysin-dependent 12- and 13-residue precursors PRA(190-201) (ELFSYLIEKVKR) and PRA(190-202) (ELFSYLIEKVKRK) directly after the epitopes C-terminal Lys198
-
-
?
FAPGNYPAL + H2O
?
show the reaction diagram
-
-
-
?
GFGDLKSPAGLQV + H2O
GFGDLK + SPAGLQV
show the reaction diagram
-
intracellular peptide identified as a substrate by incubation of HEK-293 cell extract with recombinant enzyme. Peptide is derived from eukaryotic translation elongation factor 1beta2
-
-
?
gonadotrophin-releasing hormone + H2O
?
show the reaction diagram
gonadotropin releasing hormone + H2O
?
show the reaction diagram
-
-
-
-
?
gonadotropin-releasing hormone + H2O
?
show the reaction diagram
-
-
-
-
?
gonadotropin-releasing hormone + H2O
peptide fragments
show the reaction diagram
-
-
-
?
gondotropin-releasing hormone + H2O
?
show the reaction diagram
IHSLPPEGKLG
IHSLPPE + GKLG
show the reaction diagram
-
intracellular peptide identified as a substrate by incubation of HEK-293 cell extract with recombinant enzyme. Peptide is derived from cytochrome c oxidase subunit VIII
-
-
-
KDIEDVFYKY
KDIEDVF + YKY
show the reaction diagram
-
intracellular peptide identified as a substrate by incubation of HEK-293 cell extract with recombinant enzyme. Peptide is derived from arginine/serine-rich splicing factor 1
-
-
-
LTLRTKL + H2O
LTLR + Thr + Lys-Leu
show the reaction diagram
-
at 91% of the rate with RPPGF-SP-FR
-
-
?
luliberin
peptide fragments
show the reaction diagram
-
-
-
-
?
LVVYPWTQRY + H2O
?
show the reaction diagram
-
0.72% of the activity with bradykinin, cleavage sites: LVVYP-/-W-/-T-/-Q-/-RY
-
?
major histocompatibility complex class I-presented antigenic peptides + H2O
?
show the reaction diagram
-
by destroying major histocompatibility complex class I-presented antigenic peptides the enzyme limits antigen presentation in vivo
-
?
Met-enkephalin-Arg6-Gly7-Leu8
?
show the reaction diagram
-
-
-
-
?
N-acetyl-Ala-Ala-Ala
?
show the reaction diagram
-
-
-
-
?
N-acetyl-Ala-Ala-Ala-Ala
N-acetyl-Ala + Ala-Ala-Ala
show the reaction diagram
-
-
-
?
N-succinyl-Gly-Pro-4-methylcoumaryl-7-amide + H2O
?
show the reaction diagram
-
-
-
?
neuromedin N + H2O
?
show the reaction diagram
neurotensin + H2O
?
show the reaction diagram
o-aminobenzoyl-Gly-Gly-Phe-Leu-Arg-Arg-N-(2,4-dinitrophenyl)ethylenediamine
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-Gly-Gly-Phe-Leu-Arg-Val-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
-
-
?
peptides of 9-17 residues generated by proteasomal degradation of casein + H2O
peptides of 6-9 residues
show the reaction diagram
pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 + H2O
pGlu-His-Trp-Ser-Tyr + Gly-Leu-Arg-Pro-Gly-NH2
show the reaction diagram
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg + Arg-Pro-Tyr-Ile-Leu
show the reaction diagram
phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg
?
show the reaction diagram
propionyl-Gly-Ser-Pro-(farnesyl-Cys)-Val-Leu-Met
propionyl-Gly-Ser-Pro-(farnesyl-Cys) + Val-Leu-Met
show the reaction diagram
-
-
-
?
pTLRTKL + H2O
phospho-Thr-Leu-Arg + L-tyrosine + Lys-Leu
show the reaction diagram
-
at 10% of the rate with RPPGF-SP-FR
-
-
?
PVNFKFLSH + H2O
?
show the reaction diagram
-
140% of the activity with bradykinin, cleavage sites: PVNF-/-K-/-F-/-LSH
-
?
pyroGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly + H2O
pyroGlu-His-Trp-Ser-Tyr + Gly-Leu-Arg-Pro-Gly
show the reaction diagram
pyroGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly + H2O
pyroGlu-His-Trp-Ser-Tyr + Gly-Leu-Arg-Pro-Gly + ?
show the reaction diagram
-
i.e. luteinizing hormone-releasing hormone or LHRH-I, EP24.15 can convert the decapeptide LHRH-I to the active pentapeptide LHRH-(15). EP24.15 has a biomodulating action, forming a peptide product that opposes the action of its parent peptide
i.e. luteinizing hormone-releasing hormone(1-5) or LHRH-(1-5), the fragment does not bind to LHRH-receptor instead it has antagonistic properties at the N-methyl-D-aspartic acid receptor
-
?
RGPGRAFVTI + H2O
?
show the reaction diagram
-
-
-
?
RPPGFSPFR + H2O
RPPGF + Ser-Pro + Phe-Arg
show the reaction diagram
-
-
-
-
?
SAMTEEAAVAIKAMAK + H2O
SAMTEEAAVAIK + AMAK
show the reaction diagram
-
intracellular peptide identified as a substrate by incubation of HEK-293 cell extract with recombinant enzyme. Peptide is derived from eukaryotic translation initiation factor 5A
-
-
-
SIINFEKL + H2O
?
show the reaction diagram
-
-
-
?
somatostatin + H2O
?
show the reaction diagram
succinyl-Ala-Ala-Ala-4-nitroanilide
succinyl-Ala-Ala + Ala-4-nitroanilide
show the reaction diagram
-
weak
-
?
succinyl-Ala-Ala-Phe-4-nitroanilide
succinyl-Ala-Ala + Phe-4-nitroanilide
show the reaction diagram
-
weak
-
?
succinyl-Ala-Ala-Val-4-nitroanilide
succinyl-Ala-Ala + Val-4-nitroanilide
show the reaction diagram
-
weak
-
?
tert-butoxycarbonyl-Phe-Ala-Ala-Phe-p-aminobenzoate
tert-butoxycarbonyl-Phe + Ala-Ala-Phe-p-aminobenzoate
show the reaction diagram
TPHPARIGL + H2O
?
show the reaction diagram
-
-
-
?
TYQRTRALV + H2O
?
show the reaction diagram
-
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile + H2O
Tyr-Gly-Gly-Phe-Leu + Arg-Arg-Ile
show the reaction diagram
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln + H2O
?
show the reaction diagram
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro
Tyr-Gly-Gly-Phe-Leu + Arg-Lys-Tyr-Pro
show the reaction diagram
-
beta-neoendorphin
-
?
Val-Tyr-Ile-His-Pro-Phe-His-Leu + H2O
?
show the reaction diagram
-
i.e. angiotensin I
-
-
?
vasoactive intestinal peptide
peptide fragments
show the reaction diagram
VVYPW-TQ-RY-KL + H2O
VVYPW + Thr-Gln + Arg-Tyr + Lys-Leu
show the reaction diagram
-
at 9% of the rate with RPPGF-SP-FR
-
-
?
VVYPWTQRY + H2O
?
show the reaction diagram
-
10.6% of the activity with bradykinin, cleavage sites: VVYPW-/-T-/-Q-/-RY
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-neoendorphin + H2O
?
show the reaction diagram
amyloid precursor protein + H2O
?
show the reaction diagram
-
-
-
-
?
Angiotensin I + H2O
?
show the reaction diagram
-
-
-
-
?
angiotensin I + H2O
angiotensin-(1-7) + ?
show the reaction diagram
angiotensin-I + H2O
?
show the reaction diagram
-
-
-
-
?
angiotensin-II + H2O
?
show the reaction diagram
-
-
-
-
?
beta-neoendorphin + H2O
?
show the reaction diagram
Bradykinin + H2O
?
show the reaction diagram
Collagen + H2O
?
show the reaction diagram
dynorphin 1-8 + H2O
?
show the reaction diagram
dynorphin A1-8 + H2O
?
show the reaction diagram
-
-
-
-
?
ELFADKVPKTAENFR + 2 H2O
ELFADKVPKTA + Glu-Asn + Phe-Arg
show the reaction diagram
-
intracellular peptide identified as a substrate by incubation of HEK-293 cell extract with recombinant enzyme. Peptide is derived from peptidylprolyl isomerase A
-
-
-
GFGDLKSPAGLQV + H2O
GFGDLK + SPAGLQV
show the reaction diagram
-
intracellular peptide identified as a substrate by incubation of HEK-293 cell extract with recombinant enzyme. Peptide is derived from eukaryotic translation elongation factor 1beta2
-
-
?
gonadotrophin-releasing hormone + H2O
?
show the reaction diagram
gonadotropin releasing hormone + H2O
?
show the reaction diagram
-
-
-
-
?
gonadotropin-releasing hormone + H2O
?
show the reaction diagram
-
-
-
-
?
gondotropin-releasing hormone + H2O
?
show the reaction diagram
IHSLPPEGKLG
IHSLPPE + GKLG
show the reaction diagram
-
intracellular peptide identified as a substrate by incubation of HEK-293 cell extract with recombinant enzyme. Peptide is derived from cytochrome c oxidase subunit VIII
-
-
-
KDIEDVFYKY
KDIEDVF + YKY
show the reaction diagram
-
intracellular peptide identified as a substrate by incubation of HEK-293 cell extract with recombinant enzyme. Peptide is derived from arginine/serine-rich splicing factor 1
-
-
-
major histocompatibility complex class I-presented antigenic peptides + H2O
?
show the reaction diagram
-
by destroying major histocompatibility complex class I-presented antigenic peptides the enzyme limits antigen presentation in vivo
-
?
neuromedin N + H2O
?
show the reaction diagram
neurotensin + H2O
?
show the reaction diagram
pyroGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly + H2O
pyroGlu-His-Trp-Ser-Tyr + Gly-Leu-Arg-Pro-Gly
show the reaction diagram
pyroGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly + H2O
pyroGlu-His-Trp-Ser-Tyr + Gly-Leu-Arg-Pro-Gly + ?
show the reaction diagram
-
i.e. luteinizing hormone-releasing hormone or LHRH-I, EP24.15 can convert the decapeptide LHRH-I to the active pentapeptide LHRH-(15). EP24.15 has a biomodulating action, forming a peptide product that opposes the action of its parent peptide
i.e. luteinizing hormone-releasing hormone(1-5) or LHRH-(1-5), the fragment does not bind to LHRH-receptor instead it has antagonistic properties at the N-methyl-D-aspartic acid receptor
-
?
SAMTEEAAVAIKAMAK + H2O
SAMTEEAAVAIK + AMAK
show the reaction diagram
-
intracellular peptide identified as a substrate by incubation of HEK-293 cell extract with recombinant enzyme. Peptide is derived from eukaryotic translation initiation factor 5A
-
-
-
somatostatin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
restores activity after EDTA treatment
KCl
-
strong activation
Mn2+
-
restores activity after EDTA treatment
Na2SO4
-
strong activation
NaCl
-
strong activation
NaI
-
modest activation
NH4Cl
-
modest activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(3-aminopropanoyl)-Pro-Pro-Gly-Phe-(3-aminopropanoic acid)-Pro-Phe-Arg
-
no degradation by EC 3.4.24.15
(o-aminobenzoyl)-GFSIFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
-
(o-aminobenzoyl)-GFSPPRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
-
1,10-phenanthroline
2-mercaptoethanol
-
-
5-cyano-2-nitrothiobenzoate
-
0.1 mM, 62% inhibition
-
angiotensin I
-
inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
angiotensin II
-
inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
Arg-Pro-Ala-Gly-Phe-(3-aminopropanoyl)-Pro-Phe-Arg
-
no degradation by EC 3.4.24.15
Arg-Pro-Lys-Pro
-
-
Arg-Pro-Pro-Ala-Phe-(3-aminopropanoyl)-Pro-Phe-Arg
-
low degradation by EC 3.4.24.15
Arg-Pro-Pro-Gly-Phe
-
-
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
IC50: 0.007 mM, degradation by EC 3.4.24.15
Arg-Pro-Tyr-Ile-Leu
-
-
ATP
-
Zn2+ at the active center of the enzyme is the target for the ATP binding to the enzyme leading to the peptidase inhibition and to the enzyme autophosphorylation
benzyloxycarbonyl-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Ser
Geobacillus sp.
-
-
bradykinin
-
inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
captopril
-
0.005 mM
Cbz-Phe-PSI[PO2CH2]-Ala-Lys-Ser
Geobacillus sp.
phosphinic peptide inhibitor
chymostatin
-
-
Co2+
-
10 mM, complete inhibition
cPP-Ala-Ala-Tyr-pAB
diethyl dicarbonate
-
reversed by hydroxylamine
dithiothreitol
dynorphin A1-13
-
inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
Fe2+
-
-
Fe3+
-
10 mM, complete inhibition
Gly-Pro-Phe-PSI[PO2CH2]-Gly-Pro-Nle
HVVR-4-nitroanilide
-
-
-
iodoacetate
-
1 mM, 22% inhibition
JMV390-1
L-phospho-Thr-Leu-Arg-Thr-Lys-Leu
-
comparison with inhibitory effect on EC3.4.24.16 and angiotensin-converting enzyme
Leupeptin
-
weak
LTLRTKL
-
comparison with inhibitory effect on EC3.4.24.16 and angiotensin-converting enzyme
LVVYPW-phenylThr-Gln-Arg-Tyr
-
comparison with inhibitory effect on EC3.4.24.16 and angiotensin-converting enzyme
LVVYPWTQRY
N-(1-(R,S)-carboxy-3-phenylpropyl)-L-Ala-L-Ala-L-Tyr-p-aminobenzoate
-
-
N-(1-(RS)-carboxy-3-phenylpropyl)-Ala-Aib-Tyr-p-aminobenzoate
-
-
N-(phenylethylphosphonyl)-Gly-L-Pro-L-aminohexanoic acid
-
-
N-[1(R,S)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-p-aminobenzoate
-
0.005 mM
N-[1(R,S)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate
-
the inhibitor is degraded at the Ala-Tyr bond, thus severely limiting its utility in vivo
N-[1-(R, S)-carboxy-3-phenylpropil]-Ala-Aib-Tyr-p-aminobenzoate
-
specific inhibitor JA-2
-
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-((3S)-pyrrolidine-3-carboxyl)-Phe-(3-aminopropanoic acid)
-
IC50: 0.0063 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-((3S)-pyrrolidine-3-carboxyl)-Tyr-(3-aminopropanoic acid)
-
IC50: 0.0069 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-(3-amino-2-methylpropanoyl)-((3R)-3-amino-4-phenylbutanoyl)-(3-aminopropanoic acid)
-
IC50: 0.0794 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-(3-amino-2-methylpropanoyl)-((3S)-3-amino-4-phenylbutanoyl)-(3-aminopropanoic acid)
-
IC50: 0.0631 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-(3-amino-2-methylpropanoyl)-(2-aminomethyl-3-phenylpropionyl)-(3-aminopropanoic acid)
-
IC50: 0.158 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-(3-amino-2-methylpropanoyl)-Phe-(3-aminopropanoic acid)
-
IC50: 0.0028 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-(3-amino-2-methylpropanoyl)-Tyr-3-aminopropanoic acid
-
IC50: 0.0063 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-(3-aminopropanoyl)-Tyr-(3-aminopropanoic acid)
-
IC50: 0.0056 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-(alpha-aminoisobutyryl)-Tyr-p-aminobenzoate
-
the inhibitor remains stable after 48 h with all tissues examined. The inhibitor inhibits the closely related endopeptidase EC 3.4.24.15 1/20 to 1/30 as potently as it inhibits EC 3.4.24.15
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-(piperidine-3-carboxyl)-Phe-(3-aminopropanoic acid)
-
IC50: 0.01 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-(piperidine-3-carboxyl)-Tyr-(3-aminopropanoic acid)
-
IC50: 0.0056 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Aib-Tyr-4-aminobenzoate
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Ala-((3S)-3-amino-4-(4-hydroxyphenyl)butanoyl)-(3-aminopropanoic acid)
-
IC50: 0.0251 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Ala-beta-2-Phe-(3-aminopropanoylic acid)
-
IC50: 0.04 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-(3-aminopropanoic acid)
-
IC50: 0.0036 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-pAB
-
i.e. cFP-AAF-pAB, a transition state analogue inhibitor specific for EP24.15
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-(3-aminopropanoic acid)
-
IC50: 0.00012 mM, complete degradation by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-4-aminobenzoate
-
specific inhibitor JA-2
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate
-
IC50: 0.00006 mM, potent and specific inhibitor, unstable in vivo due to cleavage between the alanine and tyrosine residues by the enzyme nephrilysin. This cleavage generates a potent inhibitor of angiotensin converting enzyme, thereby limiting the use of the inhibitor for in vivo studies
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Pro-Phe-(3-aminopropanoic acid)
-
IC50: 0.00079 mM, no cleavage by nephrilysin
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Pro-Tyr-(3-aminopropanoic acid)
-
IC50: 0.00066 mM, no cleavage by nephrilysin
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-p-aminobenzoate
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Pro-Phe-p-aminobenzoate
N-[1-(RS)-carboxyl-2-phenylethyl]-Ala-Ala-Phe-4-amino-benzoate
-
i.e. cFP-AAF-pAB, an active site-directed inhibitor
Ni2+
-
-
o-phenanthroline
p-hydroxymercuribenzoate
phosphodiepryl 08
phosphodiepryl 21
phosphodiepryl 33
phosphoramidon
-
-
Pro-Ile
-
13% inhibition at 1 mM
PVNFKFLSH
-
inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)
RPPG-((3R)-3-amino-4-phenylbutanoic acid)-SPFR
-
IC50: 0.012 mM; no degradation by EC 3.4.24.15
RPPG-((3S)-3-amino-4-phenylbutanoic acid)-SPFR
-
IC50: 0.02 mM; no degradation by EC 3.4.24.15
RPPG-(2-aminomethyl-3-phenylpropionic acid)-SPFR
-
no degradation by EC 3.4.24.15
RPPGF-((3R)-3-amino-4-hydroxybutanoic acid)-PFR
-
no degradation by EC 3.4.24.15
S-nitroso-N-acetylpenicillamine
-
inhibition is prevented by DTT
Ser-Pro-Phe-Arg
-
-
tosyl-Phe-CH3Cl
-
0.1 mM, 15% inactivation
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys
VVYPW-phenylThr-Gln-Arg-Tyr
-
comparison with inhibitory effect on EC3.4.24.16 and angiotensin-converting enzyme
VVYPWTQRY
Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Ser
Geobacillus sp.
-
specific for thimet oligopeptidase
Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Tyr
Geobacillus sp.
-
specific for thimet oligopeptidase
Z-Pro-prolinal
-
12% inhibition at 0.001 mM
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
Calmodulin
-
interaction with calmodulin is important for the secretion of thimet oligopeptidase following stimulation. Overexpression of calmodulin in HEK293 cells is sufficient to greatly increase the A23187-stimulated secretion of EP24.15, which can be inhibited by the calmodulin inhibitor calmidazolium
Calmodulin/Ca2+
-
Ca2+-dependent interaction with calmodulin is important for the secretion of thimet oligopeptidase following stimulation. Overexpression of calmodulin in HEK293 cells is sufficient to greatly increase the A23187-stimulated secretion of EP24.15, which can be inhibited by the calmodulin inhibitor calmidazolium
-
cocaine
-
cocaine treatment causes an increase in the ventral striatum in enzyme specific activity and enzyme relative mRNA amount determined by real time RT-PCR
dithiothreitol
GSSG
-
enzyme oligomerization, induced by S-glutathionylation, has a regulatory function, maximal activity by partial S-glutathionylation, overview
thiol
-
stimulation
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.014 - 0.037
(7-methoxycoumarin-4-yl)acetyl-Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-OH
0.0025
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl
-
recombinant enzyme from E. coli
0.0014
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl
-
recombinant enzyme from E. coli
0.0012 - 0.0057
(7-methoxycoumarin-4-yl)acetyl-Leu-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH
0.0045
(o-aminobenzoyl)-AFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.003
(o-aminobenzoyl)-DFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0012
(o-aminobenzoyl)-EFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.001
(o-aminobenzoyl)-FFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0042
(o-aminobenzoyl)-GASPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0166
(o-aminobenzoyl)-GDSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0225
(o-aminobenzoyl)-GESPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00071
(o-aminobenzoyl)-GFAPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0068
(o-aminobenzoyl)-GFDPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0052
(o-aminobenzoyl)-GFEPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0014
(o-aminobenzoyl)-GFFPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00072
(o-aminobenzoyl)-GFHPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00099
(o-aminobenzoyl)-GFIPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00038
(o-aminobenzoyl)-GFLPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00027
(o-aminobenzoyl)-GFNPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.006
(o-aminobenzoyl)-GFPPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0149
(o-aminobenzoyl)-GFQPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0053
(o-aminobenzoyl)-GFRPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0012
(o-aminobenzoyl)-GFSAFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0076
(o-aminobenzoyl)-GFSDFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0038
(o-aminobenzoyl)-GFSEFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00069
(o-aminobenzoyl)-GFSFFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0011
(o-aminobenzoyl)-GFSHFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0016
(o-aminobenzoyl)-GFSLFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
0.004
(o-aminobenzoyl)-GFSPARQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.037
(o-aminobenzoyl)-GFSPDRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.018
(o-aminobenzoyl)-GFSPERQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.002
(o-aminobenzoyl)-GFSPFAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0139
(o-aminobenzoyl)-GFSPFDQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.119
(o-aminobenzoyl)-GFSPFEQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0019
(o-aminobenzoyl)-GFSPFFQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0126
(o-aminobenzoyl)-GFSPFHQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0017
(o-aminobenzoyl)-GFSPFIQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0044
(o-aminobenzoyl)-GFSPFLQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.131
(o-aminobenzoyl)-GFSPFNQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0203
(o-aminobenzoyl)-GFSPFPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0038
(o-aminobenzoyl)-GFSPFQQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0048
(o-aminobenzoyl)-GFSPFRA-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0012
(o-aminobenzoyl)-GFSPFRE-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00069
(o-aminobenzoyl)-GFSPFRF-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0005
(o-aminobenzoyl)-GFSPFRI-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00057
(o-aminobenzoyl)-GFSPFRL-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0018
(o-aminobenzoyl)-GFSPFRN-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0059
(o-aminobenzoyl)-GFSPFRP-(N-(2,4-dinitrophenyl)ethylenediamine)
-
-
0.0022
(o-aminobenzoyl)-GFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00019
(o-aminobenzoyl)-GFSPFRR-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0043
(o-aminobenzoyl)-GFSPFRS-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.007
(o-aminobenzoyl)-GFSPFSQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0015
(o-aminobenzoyl)-GFSPHRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0085
(o-aminobenzoyl)-GFSPIRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0045
(o-aminobenzoyl)-GFSPLRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.005
(o-aminobenzoyl)-GFSPNRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0043
(o-aminobenzoyl)-GFSPQRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0018
(o-aminobenzoyl)-GFSPRRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0073
(o-aminobenzoyl)-GFSPSRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0075
(o-aminobenzoyl)-GFSQFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0022
(o-aminobenzoyl)-GFSRFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0037
(o-aminobenzoyl)-GFSSFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0012
(o-aminobenzoyl)-GFSXFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0005
(o-aminobenzoyl)-GGFLRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0033
(o-aminobenzoyl)-GGFLRRDQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0019
(o-aminobenzoyl)-GGFLRREQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0007
(o-aminobenzoyl)-GGFLRRFQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0017
(o-aminobenzoyl)-GGFLRRHQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0018
(o-aminobenzoyl)-GGFLRRIQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0007
(o-aminobenzoyl)-GGFLRRLQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0015
(o-aminobenzoyl)-GGFLRRNQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0012
(o-aminobenzoyl)-GGFLRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0023
(o-aminobenzoyl)-GGFLRRRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0022
(o-aminobenzoyl)-GGFLRRV-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0021
(o-aminobenzoyl)-GGFLRRVQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0006
(o-aminobenzoyl)-GGFLRRYQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4. 37C
0.0048
(o-aminobenzoyl)-GHSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0028
(o-aminobenzoyl)-GISPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0037
(o-aminobenzoyl)-GLSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0045
(o-aminobenzoyl)-GNSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0037
(o-aminobenzoyl)-GPSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0099
(o-aminobenzoyl)-GQSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0036
(o-aminobenzoyl)-GRSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.006
(o-aminobenzoyl)-GSSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0017
(o-aminobenzoyl)-HFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00016
(o-aminobenzoyl)-IFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00082
(o-aminobenzoyl)-LFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.00078
(o-aminobenzoyl)-NFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0015
(o-aminobenzoyl)-QFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
0.0014
(o-aminobenzoyl)-SFSPFRQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 7.4, 37C
0.0102
(ortho-aminobenzoyl)-AKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.021
(ortho-aminobenzoyl)-ENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.0028
(ortho-aminobenzoyl)-GGFLPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.114
(ortho-aminobenzoyl)-KPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.007
(ortho-aminobenzoyl)-LYENKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.0071
(ortho-aminobenzoyl)-NAPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.064
(ortho-aminobenzoyl)-NKARRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.036
(ortho-aminobenzoyl)-NKPARPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.015
(ortho-aminobenzoyl)-NKPRAPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.0124
(ortho-aminobenzoyl)-NKPRRAQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.0121
(ortho-aminobenzoyl)-NKPRRPQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
pH 8.0, 37C, hydrolysis of R-R bond
0.0137
2,4-dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
-
0.0015 - 0.0043
2-aminobenzoyl-GFSPFRQ-(N-2,4-dinitrophenyl)ethylenediamine
0.0123
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-(2,4-ditritrophenyl)Lys
-
-
-
0.0082
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)
Geobacillus sp.
pH and temperature not specified in the publication
0.009 - 0.0343
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
0.000057 - 0.00127
7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin
0.00401 - 0.009
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol
0.004 - 0.01
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl
0.000041 - 0.0021
7-methoxycoumarin-4-acetyl-[Ala7, Lys(dinitrophenol)9]-bradykinin
0.0143
Abz-GFSAFRQ-EDDnp
-
pH 7.4, 37C
0.0012 - 0.0098
Abz-GFSAFRQEDDnp
0.0009
Abz-GFSDFRQ-EDDnp
-
pH 7.4, 37C
0.0011
Abz-GFSEFRQ-EDDnp
-
pH 7.4, 37C
0.0021 - 0.0075
Abz-GFSEFRQEDDnp
0.0105
Abz-GFSFFRQ-EDDnp
-
pH 7.4, 37C
0.0007 - 0.016
Abz-GFSFFRQEDDnp
0.0321
Abz-GFSHFRQ-EDDnp
-
pH 7.4, 37C
0.0076 - 0.0096
Abz-GFSHFRQEDDnp
0.112
Abz-GFSIFRQ-EDDnp
-
pH 7.4, 37C
0.0004 - 0.005
Abz-GFSIFRQEDDnp
0.0016 - 0.0102
Abz-GFSLFRQEDDnp
0.0218
Abz-GFSPARQ-EDDnp
-
pH 7.4, 37C
0.0075
Abz-GFSPDRQ-EDDnp
-
pH 7.4, 37C
0.0192
Abz-GFSPERQ-EDDnp
-
pH 7.4, 37C
0.0093
Abz-GFSPFRQ-EDDnp
-
pH 7.4, 37C
0.0131
Abz-GFSPHRQ-EDDnp
-
pH 7.4, 37C
0.0062
Abz-GFSPIRQ-EDDnp
-
pH 7.4, 37C
0.0122
Abz-GFSPLRQ-EDDnp
-
pH 7.4, 37C
0.0148
Abz-GFSPQRQ-EDDnp
-
pH 7.4, 37C
0.0213
Abz-GFSPRRQ-EDDnp
-
pH 7.4, 37C
0.0135
Abz-GFSPSRQ-EDDnp
-
pH 7.4, 37C
0.0091
Abz-GFSQFRQ-EDDnp
-
pH 7.4, 37C
0.0012 - 0.0058
Abz-GFSQFRQEDDnp
0.0109
Abz-GFSRFRQ-EDDnp
-
pH 7.4, 37C
0.0011 - 0.01
Abz-GFSRFRQEDDnp
0.0127
Abz-GFSSFRQ-EDDnp
-
pH 7.4, 37C
0.0037 - 0.02
Abz-GFSSFRQEDDnp
0.0208
Abz-GFSWFRQ-EDDnp
-
pH 7.4, 37C
0.0008 - 0.0074
Abz-GFSWFRQEDDnp
0.0009 - 0.011
Abz-GFSyFRQEDDnp
0.067
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
-
3.1 - 3.45
benzoyl-Gly-Ala-Ala-Gly-p-aminobenzoate
1.54 - 2.5
benzoyl-Gly-Ala-Ala-Leu-p-aminobenzoate
0.058 - 0.83
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
0.37
benzoyl-Gly-Arg-Ala-Ala-Phe-p-aminobenzoate
-
-
0.72
benzoyl-Gly-Asp-Ala-Ala-Phe-p-aminobenzoate
-
-
1.36 - 2
benzoyl-Gly-Gly-Ala-Phe-p-aminobenzoate
0.24
benzoyl-Gly-Lys-Arg-Ala-Ala-Phe-p-aminobenzoate
-
-
0.16
benzoyl-Gly-Phe-Ala-Ala-Phe-p-aminobenzoate
-
-
0.39 - 0.51
benzoyl-Gly-Phe-Ala-Phe-p-aminobenzoate
0.031
benzoyl-Gly-Phe-Phe-Ala-Ala-Phe-p-aminobenzoate
-
-
0.38
bradykinin
-
-
0.012 - 0.0161
dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
0.0732
Gly-Pro-Leu
-
-
0.048
Gly-Pro-Leu-Gly-Pro-Leu
-
-
0.0149
Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu
-
-
0.0135
Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu
-
-
0.0154
Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu
-
-
0.0026 - 1.64
neurotensin
0.095
pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
-
-
0.037
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
-
-
32.5
propionyl-Gly-Ser-Pro-(farnesyl-Cys)-Val-Leu-Met
-
-
0.1 - 0.22
tert-butoxycarbonyl-Phe-Ala-Ala-Phe-p-aminobenzoate
0.06
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
-
-
0.038
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro
-
-
additional information
additional information
-
kinetics of wild-type and mutant TOP
-