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Literature summary for 3.4.24.15 extracted from

  • Barrett, A.J.; Brown, M.A.; Dando, P.M.; Knight, C.G.; McKie, N.; Rawlings, N.D.; Serizawa, A.
    Thimet oligopeptidase and oligopeptidase M or neurolysin (1995), Methods Enzymol., 248, 529-556.
    View publication on PubMed
Search for more literature for 3.4.24.15

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol enzyme that has been exposed to air in solution lacking thiol compounds shows very marked stimulation of activity by low concentrations of 2-mercaptoethanol Homo sapiens
2-mercaptoethanol enzyme that has been exposed to air in solution lacking thiol compounds shows very marked stimulation of activity by low concentrations of 2-mercaptoethanol Rattus norvegicus
dithiothreitol enzyme that has been exposed to air in solution lacking thiol compounds shows very marked stimulation of activity by low concentrations of dithiothreitol Homo sapiens
dithiothreitol enzyme that has been exposed to air in solution lacking thiol compounds shows very marked stimulation of activity by low concentrations of dithiothreitol Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 10 mM, reversible Homo sapiens
dithiothreitol 10 mM, reversible Rattus norvegicus
EDTA
-
 Homo sapiens
EDTA reactivation by metal ions Rattus norvegicus
Hg2+
-
 Homo sapiens
Hg2+
-
 Rattus norvegicus
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-p-aminobenzoate
-
 Homo sapiens
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Phe-p-aminobenzoate
-
 Rattus norvegicus
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate
-
 Rattus norvegicus
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Pro-Phe-p-aminobenzoate
-
 Homo sapiens
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Pro-Phe-p-aminobenzoate
-
 Rattus norvegicus
p-hydroxymercuribenzoate
-
 Homo sapiens
p-hydroxymercuribenzoate
-
 Rattus norvegicus
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys i.e. dynorphin-(1-13) Homo sapiens
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys i.e. dynorphin-(1-13) Rattus norvegicus
Zn2+ above 0.1 mM Homo sapiens
Zn2+ above 0.1 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl recombinant enzyme from E. coli Rattus norvegicus
0.0025
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl recombinant enzyme from E. coli Rattus norvegicus
0.009
-
 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
-
 Homo sapiens
0.012
-
 dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Rattus norvegicus
0.0133
-
 dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Homo sapiens
0.0161
-
 dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys recombinant enzyme from E. coli Rattus norvegicus
0.023
-
 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
-
 Rattus norvegicus
0.0343
-
 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl recombinant enzyme from E. coli Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc contains 2 gatom of zinc per mol of enzyme, one catalytic zinc and one structural zinc Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78300
-
 x * 78300, calculation from nucleotide sequence Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens proposed role in formation of enkephalins ?
-
 ?
additional information Rattus norvegicus proposed role in formation of enkephalins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-
hypophysis
-
 Rattus norvegicus
-
testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile-Arg-Arg-Ala-Lys-dinitrophenyl
-
 Rattus norvegicus (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Ile + Arg-Arg-Ala-Lys-dinitrophenyl
-
 ?
(7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu-Arg-Arg-Ala-Lys-dinitrophenyl
-
 Rattus norvegicus (7-methoxycoumarin-4-yl)acetyl-Gly-Gly-Phe-Leu + Arg-Arg-Ala-Lys-dinitrophenyl
-
 ?
4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg
-
 Homo sapiens 4-phenylazobenzyloxycarbonyl-Pro-Leu + Gly-Pro-Arg
-
 ?
4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg
-
 Rattus norvegicus 4-phenylazobenzyloxycarbonyl-Pro-Leu + Gly-Pro-Arg
-
 ?
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
-
 Homo sapiens 7-methoxycoumarin-3-carboxylyl-Pro-Leu + Gly-Pro-D-Lys-dinitrophenyl
-
 ?
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl
-
 Rattus norvegicus 7-methoxycoumarin-3-carboxylyl-Pro-Leu + Gly-Pro-D-Lys-dinitrophenyl
-
 ?
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
 Homo sapiens benzoyl-Gly + Ala-Ala-Phe-p-aminobenzoate
-
 ?
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
 Rattus norvegicus benzoyl-Gly + Ala-Ala-Phe-p-aminobenzoate
-
 ?
dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Homo sapiens dinitrophenyl-Pro-Leu + Gly-Pro-Trp-D-Lys
-
 ?
dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Rattus norvegicus dinitrophenyl-Pro-Leu + Gly-Pro-Trp-D-Lys
-
 ?
additional information proposed role in formation of enkephalins Homo sapiens ?
-
 ?
additional information proposed role in formation of enkephalins Rattus norvegicus ?
-
 ?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu i.e. neurotensin Homo sapiens pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg + Arg-Pro-Tyr-Ile-Leu
-
 ?
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu i.e. neurotensin Rattus norvegicus pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg + Arg-Pro-Tyr-Ile-Leu
-
 ?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln + H2O dynorphin 1-17 Homo sapiens ?
-
 ?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln + H2O dynorphin 1-17 Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
monomer x * 78300, calculation from nucleotide sequence Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 above Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 unstable above Rattus norvegicus