KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000057 | - |
7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin | pH 7.8, 23°C | Rattus norvegicus | |
0.00013 | - |
7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin | pH 7.8, 23°C, presence of 1.5 M urea | Rattus norvegicus | |
0.00048 | - |
7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin | pH 7.8, 23°C, presence of 2.5 M urea | Rattus norvegicus | |
0.00127 | - |
7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin | pH 7.8, 23°C, presence of 3.5 M urea | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | second step of structural unfolding results in complete loss of catalytic Zn2+ | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P24155 | - |
- |
Renatured (Comment) | Organism |
---|---|
thermal unfolding is a two-step process with a major endotherm at a Tm of 64°C and a minor endotherm at a Tm of 55°C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin + H2O | - |
Rattus norvegicus | ? | - |
? | |
additional information | conformational changes due to chemical denaturation are directly correlated with a change in substrate specificity | Rattus norvegicus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin | pH 7.8, 23°C | Rattus norvegicus | |
0.72 | - |
7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin | pH 7.8, 23°C, presence of 1.5 M urea | Rattus norvegicus | |
0.91 | - |
7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin | pH 7.8, 23°C, presence of 2.5 M urea | Rattus norvegicus | |
1.07 | - |
7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin | pH 7.8, 23°C, presence of 3.5 M urea | Rattus norvegicus |