Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.15 extracted from

  • Oliveira, V.; Garrido, P.A.; Rodrigues, C.C.; Colquhoun, A.; Castro, L.M.; Almeida, P.C.; Shida, C.S.; Juliano, M.A.; Juliano, L.; Camargo, A.C.; Hyslop, S.; Roberts, J.L.; Grum-Tokars, V.; Glucksman, M.J.; Ferro, E.S.
    Calcium modulates endopeptidase 24.15 (EC 3.4.24.15) membrane association, secondary structure and substrate specificity (2005), FEBS J., 272, 2978-2992.
    View publication on PubMed
Search for more literature for 3.4.24.15

Protein Variants

Protein Variants Comment Organism
D159A similar to wild-type regarding enzymatic activity, secondary structure, calcium sensitivity and immunoreactivity Rattus norvegicus
D93A similar to wild-type regarding enzymatic activity, secondary structure, calcium sensitivity and immunoreactivity. Reduced ability to associate with the plasma membrane Rattus norvegicus
D93A/D153A similar to wild-type regarding enzymatic activity, secondary structure, calcium sensitivity and immunoreactivity. Reduced ability to associate with the plasma membrane Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ extracellular Ca2+ concentration has no effect on the total amount of extracellular enzyme. Absence of Ca2+ favors the enzyme shedding from the plasma membrane into the medium. In presence of Ca2+, change of preference to R-R bonds instead of L-R bonds of substrates 2-aminobenzoyl-GGFLRRVQ-(N-(2,4-dinitrophenyl)-ethylenediamine) and 2-aminobenzoyl-GGFLRRDQ-(N-(2,4-dinitrophenyl)-ethylenediamine) Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 x * 78000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-GGFLRRDQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O absence of Ca2+, 37% cleavage of R-R bond and 63% cleavage of L-R bond. Presence of 50 mM Ca2+, 74% cleavage of R-R bond and 26% of L-R bond Rattus norvegicus 2-aminobenzoyl-GGFL + RRDQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + 2-aminobenzoyl-GGFLR + RDQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
2-aminobenzoyl-GGFLRRVQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Rattus norvegicus 2-aminobenzoyl-GGFL + RRVQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + 2-aminobenzoyl-GGFLR + RVQ-(N-(2,4-dinitrophenyl)-ethylenediamine) absence of Ca2+, 64% cleavage of R-R bond and 36% cleavage of L-R bond. Presence of 50 mM Ca2+, 79% cleavage of R-R bond and 21% of L-R bond ?

Subunits

Subunits Comment Organism
? x * 78000, SDS-PAGE Rattus norvegicus