Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.15 extracted from

  • Machado, M.F.; Marcondes, M.F.; Rioli, V.; Ferro, E.S.; Juliano, M.A.; Juliano, L.; Oliveira, V.
    Catalytic properties of thimet oligopeptidase H600A mutant (2010), Biochem. Biophys. Res. Commun., 394, 429-433.
    View publication on PubMed
Search for more literature for 3.4.24.15

Protein Variants

Protein Variants Comment Organism
H600A the mutation affects both Km and kcat, the mutant shows changes in the pH-profile, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Aib-Tyr-4-aminobenzoate i.e. JA-2, a potent TOP inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of wild-type and mutant TOP Homo sapiens
0.0009
-
 Abz-GFSDFRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0011
-
 Abz-GFSEFRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0062
-
 Abz-GFSPIRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0075
-
 Abz-GFSPDRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0091
-
 Abz-GFSQFRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0093
-
 Abz-GFSPFRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0105
-
 Abz-GFSFFRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0109
-
 Abz-GFSRFRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0122
-
 Abz-GFSPLRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0127
-
 Abz-GFSSFRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0131
-
 Abz-GFSPHRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0135
-
 Abz-GFSPSRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0143
-
 Abz-GFSAFRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0148
-
 Abz-GFSPQRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0192
-
 Abz-GFSPERQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0208
-
 Abz-GFSWFRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0213
-
 Abz-GFSPRRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0218
-
 Abz-GFSPARQ-EDDnp pH 7.4, 37°C Homo sapiens
0.0321
-
 Abz-GFSHFRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.112
-
 Abz-GFSIFRQ-EDDnp pH 7.4, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-GFSAFRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSA + FRQ-EDDnp
-
 ?
Abz-GFSDFRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSD + FRQ-EDDnp
-
 ?
Abz-GFSEFRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSE + FRQ-EDDnp
-
 ?
Abz-GFSFFRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSF + FRQ-EDDnp
-
 ?
Abz-GFSHFRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSH + FRQ-EDDnp
-
 ?
Abz-GFSIFRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSI + FRQ-EDDnp
-
 ?
Abz-GFSPARQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSP + Ala-Arg-Gln-EDDnp
-
 ?
Abz-GFSPDRQ-EDDnp + 2 H2O
-
 Homo sapiens Abz-Gly-Phe + Ser-Pro + Asp-Arg-Gln-EDDnp
-
 ?
Abz-GFSPERQ-EDDnp + 2 H2O
-
 Homo sapiens Abz-Gly-Phe + Ser-Pro + Glu-Arg-Gln-EDDnp
-
 ?
Abz-GFSPFRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSP + FRQ-EDDnp
-
 ?
Abz-GFSPHRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSP + His-Arg-Gln-EDDnp
-
 ?
Abz-GFSPIRQ-EDDnp + 2 H2O
-
 Homo sapiens Abz-Gly-Phe + Ser-Pro + Ile-Arg-Gln-EDDnp
-
 ?
Abz-GFSPLRQ-EDDnp + 2 H2O
-
 Homo sapiens Abz-Gly-Phe + Ser-Pro + Leu-Arg-Gln-EDDnp
-
 ?
Abz-GFSPQRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSP + Gln-Arg-Gln-EDDnp
-
 ?
Abz-GFSPRRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSP + Arg-Arg-Gln-EDDnp
-
 ?
Abz-GFSPSRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSP + Ser-Arg-Gln-EDDnp
-
 ?
Abz-GFSQFRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSQ + FRQ-EDDnp
-
 ?
Abz-GFSRFRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSR + FRQ-EDDnp
-
 ?
Abz-GFSSFRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSS + FRQ-EDDnp
-
 ?
Abz-GFSWFRQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSW + FRQ-EDDnp
-
 ?
additional information substrate specificity, no activity with Abz-GFSLFRQ-EDDnp, Abz-GFSPPRQ-EDDnp, and Abz-GFSPWRQ-EDDnp, overview. Importance of His600 for both substrate binding and/or product release from active site, participation of His600 in TOP catalysis, transferring a proton to the newly generated NH2-terminus or helping Tyr605 and/or Tyr612 in the intermediate oxyanion stabilization Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme is a member of M3 metallopeptidase family that contains the HEXXH motif Homo sapiens
TOP
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 Abz-GFSPERQ-EDDnp pH 7.4, 37°C Homo sapiens
0.02
-
 Abz-GFSPLRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.03
-
 Abz-GFSPDRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.04
-
 Abz-GFSPIRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.19
-
 Abz-GFSIFRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.3
-
 Abz-GFSDFRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.3
-
 Abz-GFSPHRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.4
-
 Abz-GFSPSRQ-EDDnp pH 7.4, 37°C Homo sapiens
0.7
-
 Abz-GFSPQRQ-EDDnp pH 7.4, 37°C Homo sapiens
1.6
-
 Abz-GFSEFRQ-EDDnp pH 7.4, 37°C Homo sapiens
1.7
-
 Abz-GFSPARQ-EDDnp pH 7.4, 37°C Homo sapiens
2.9
-
 Abz-GFSPFRQ-EDDnp pH 7.4, 37°C Homo sapiens
4.5
-
 Abz-GFSPRRQ-EDDnp pH 7.4, 37°C Homo sapiens
5.9
-
 Abz-GFSFFRQ-EDDnp pH 7.4, 37°C Homo sapiens
6
-
 Abz-GFSWFRQ-EDDnp pH 7.4, 37°C Homo sapiens
7
-
 Abz-GFSQFRQ-EDDnp pH 7.4, 37°C Homo sapiens
9.1
-
 Abz-GFSRFRQ-EDDnp pH 7.4, 37°C Homo sapiens
9.2
-
 Abz-GFSSFRQ-EDDnp pH 7.4, 37°C Homo sapiens
11.1
-
 Abz-GFSAFRQ-EDDnp pH 7.4, 37°C Homo sapiens
13.3
-
 Abz-GFSHFRQ-EDDnp pH 7.4, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 pH-profiles of TOP H600A mutant and wild-type enzyme Homo sapiens

General Information

General Information Comment Organism
metabolism thimet oligopeptidase is a metallo-oligopeptidase that participates in the intracellular metabolism of peptides Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
 Abz-GFSPERQ-EDDnp pH 7.4, 37°C Homo sapiens
2
-
 Abz-GFSIFRQ-EDDnp pH 7.4, 37°C Homo sapiens
8
-
 Abz-GFSPLRQ-EDDnp pH 7.4, 37°C Homo sapiens
8
-
 Abz-GFSPDRQ-EDDnp pH 7.4, 37°C Homo sapiens
10
-
 Abz-GFSPIRQ-EDDnp pH 7.4, 37°C Homo sapiens
20.6
-
 Abz-GFSPHRQ-EDDnp pH 7.4, 37°C Homo sapiens
30
-
 Abz-GFSPSRQ-EDDnp pH 7.4, 37°C Homo sapiens
47
-
 Abz-GFSPQRQ-EDDnp pH 7.4, 37°C Homo sapiens
78
-
 Abz-GFSPARQ-EDDnp pH 7.4, 37°C Homo sapiens
211
-
 Abz-GFSPRRQ-EDDnp pH 7.4, 37°C Homo sapiens
288
-
 Abz-GFSWFRQ-EDDnp pH 7.4, 37°C Homo sapiens
317
-
 Abz-GFSPFRQ-EDDnp pH 7.4, 37°C Homo sapiens
333
-
 Abz-GFSDFRQ-EDDnp pH 7.4, 37°C Homo sapiens
414
-
 Abz-GFSHFRQ-EDDnp pH 7.4, 37°C Homo sapiens
561
-
 Abz-GFSFFRQ-EDDnp pH 7.4, 37°C Homo sapiens
724
-
 Abz-GFSSFRQ-EDDnp pH 7.4, 37°C Homo sapiens
771
-
 Abz-GFSQFRQ-EDDnp pH 7.4, 37°C Homo sapiens
839
-
 Abz-GFSRFRQ-EDDnp pH 7.4, 37°C Homo sapiens
1417
-
 Abz-GFSEFRQ-EDDnp pH 7.4, 37°C Homo sapiens
1600
-
 Abz-GFSAFRQ-EDDnp pH 7.4, 37°C Homo sapiens